Search results for "Synuclein"

showing 2 items of 42 documents

Neuroprotective effects of antibodies on retinal ganglion cells in an adolescent retina organ culture

2016

Glaucoma, a neurodegenerative disease, is characterized by a progressive loss of retinal ganglion cells (rgc). Up- and down-regulated autoantibody immunoreactivities in glaucoma patients have been demonstrated. Previous studies showed protective effects of down-regulated antibodies [gamma (γ)-synuclein and glial fibrillary acidic protein [GFAP]) on neuroretinal cells. The aim of this study was to test these protective antibody effects on rgc in an organ culture model and to get a better understanding of cell-cell interactions of the retina in the context of the protective effect. We used an adolescent retinal organ culture (pig) with an incubation time of up to 4 days. Retinal explants were…

MaleRetinal Ganglion Cells0301 basic medicinePathologymedicine.medical_specialtyAdolescentgenetic structuresSwineNerve Tissue ProteinsContext (language use)Organ cultureBiochemistryRetinal ganglionAntibodies03 medical and health sciencesCellular and Molecular Neurosciencechemistry.chemical_compoundOrgan Culture TechniquesGlutamate-Ammonia LigaseGlutamine synthetaseGlial Fibrillary Acidic ProteinmedicineAnimalsHumansRetinaGlial fibrillary acidic proteinbiologyMyoglobinGlaucomaRetinalEndoplasmic Reticulum StressImmunohistochemistryMolecular biologyeye diseasesNeuroprotective Agents030104 developmental biologymedicine.anatomical_structurechemistryalpha-Synucleinbiology.proteinImmunohistochemistryFemalesense organsJournal of Neurochemistry
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Prosurvival effect of human wild-type alpha-synuclein on MPTP-induced toxicity to central but not peripheral catecholaminergic neurons isolated from …

2010

In the present work we report the generation of a new line of alpha-synuclein (alpha-SYN) transgenic mice in which the human wild-type alpha-SYN cDNA is expressed under the control of a tyrosine hydroxylase (TH) promoter. We provide evidence that the ectopic protein is found in TH expressing neurons of both central and peripheral nervous systems. The transgene is expressed very early in development coinciding with the activity of the TH promoter and in the adult brain the human protein distributes normally to the nerve endings and cell bodies of dopaminergic nigral neurons without any evidence of abnormal aggregation. Our results indicate that expression of human wild-type alpha-SYN does no…

medicine.medical_specialtySympathetic Nervous SystemTyrosine 3-MonooxygenaseTransgeneMice Transgenicchemistry.chemical_compoundMiceCatecholaminesDopamineMesencephalonInternal medicinemedicineNeurotoxinAnimalsHumansTransgenesPromoter Regions GeneticCells CulturedDopamine transporterNeuronsDopamine Plasma Membrane Transport ProteinsTyrosine hydroxylasebiologyCell DeathGeneral NeuroscienceMPTPDopaminergicBrainEndocrinologynervous systemchemistry1-Methyl-4-phenyl-1236-tetrahydropyridineOrgan Specificitybiology.proteinalpha-SynucleinCatecholaminergic cell groupsmedicine.drugNeuroscience
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