Search results for "T-RFLP"

Cause and duration of mustard incorporation effects on soil-borne plant pathogenic fungi

2009

International audience; Two fungal plant pathogens, Rhizoctonia solani AG 2-2 and Fusarium oxysporum f.sp. lini, were studied in relation to general responses of soil fungi and bacteria following incorporation of Brassica juncea. Our aim was to understand to what extent the changes in the biological and physicochemical characteristics of the soil could explain the effects on the studied pathogens and diseases, and to determine the temporal nature of the responses. Short-term effects of mustard incorporation (up to 4 months) were investigated in a microcosm experiment, and compared with a treatment where composted plant material was incorporated. In a field experiment, the responses were fol…

Effects of the introduction of a biocontrol strain of Trichoderma atroviride on non target soil micro-organisms

2009

International audience; The main objective of this study was to assess the impact of the application of an antagonistic strain of Trichoderma atroviride on the native microbial soil communities. The structures of the fungal and bacterial communities were assessed by T-RFLP (terminal restriction fragment length polymorphism) method, based on T-RFLP analysis of 18S and 16S rRNA genes, respectively. Results showed that the introduction of the strain I-1237 into two soils slightly modified the microbial diversity, only for a short period of time. Nine months post-inoculation resilience took place, resulting in similar structures of the fungal and bacterial communities in the inoculated and cont…

Spatial microbial community structure and biodiversity analysis in "extreme" hypersaline soils of a semiarid Mediterranean area

2015

In recent years specific attention has been paid on the biotechnological potential of microorganisms in extreme soils, in particular in saline soils. Salinity is one of the most widespread soil degradation processes on the Earth, and saline soils can be defined as extreme soils or border line habitats in which several factors, as high salt content, may limit the growth of organisms. In this study, the physical, chemical and microbiological soil properties were investigated in the shallower horizon of natural saltaffected soils in Sicily (Italy). The main aim of the research was to evaluate the structure and diversity of bacterial and archaeal communities by terminal-restriction fragment len…

Community Structure of Methanogens in the Hydrolytic Reactors of Two-Stage Anaerobic Biogas Reactor

2008

Impact d'amendements organiques sur la structure des communautés microbienne des sols : Choix des méthodes, validation et résultats

2009

L'origine de toutes les altérations superficielles du tubercule de pomme de terre est-elle vraiment infectieuse?

2009

SPE communication orale, résumé; Deux essais en sol stérile testant le pouvoir pathogène des souches le plus fréquemment isolées et inoculées seules, et un essai en hydroponie testant l'effet de co-inoculations de 2 souches sur l'apparition des altérations ont été mis en place.