Search results for "TECNOLOGICO"

showing 10 items of 440 documents

Fluorinated and pegylated polyaspartamide derivatives to increase solubility and efficacy of Flutamide

2012

New fluorinated amphiphilic copolymers based on a biocompatible polyaspartamide have been prepared in order to obtain polymeric micelles useful for delivering anticancer drugs. In particular, α,β-poly(N-2-hydroxyethyl)-d,l-aspartamide (PHEA) has been derivatized with polyethylene glycol (PEG(2000)) and ethylendiamine (EDA). Both these portions form the hydrophilic part of the copolymer, while the hydrophobic moiety is given by 1,2,4-oxadiazoles: 5-pentafluorophenyl-3-perfluoroheptyl-1,2,4-oxadiazole (PPOX) or 3-carboxyethyl-5-pentadecafluoroheptyl-1,2,4-oxadiazole (CPOX). Copolymers named PHEA-PEG(2000)-EDA-PPOX and PHEA-PEG(2000)-EDA-CPOX have been prepared with various degrees of derivati…

MaleAntineoplastic Agents HormonalPolymersSize-exclusion chromatographyPharmaceutical SciencePolyethylene glycolAdenocarcinomaPolyethylene Glycolschemistry.chemical_compoundDrug Delivery SystemsCell Line TumorPolymer chemistryCopolymerHumansSolubilityDerivatizationMicellesCell Proliferationchemistry.chemical_classificationDrug CarriersOxadiazolesProstatic NeoplasmsDihydrotestosteroneSettore CHIM/06 - Chimica OrganicaPolymerEthylenediaminesFlutamideCancer targeting cell model colloidal particles drug delivery polymerSolubilitychemistrySettore CHIM/09 - Farmaceutico Tecnologico ApplicativoDrug deliveryChromatography GelMicroscopy Electron ScanningPyrenePeptidesHydrophobic and Hydrophilic InteractionsJournal of Drug Targeting
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SYNTHESIS, PHYSICO-CHEMICAL AND BIOLOGICAL CHARACTERIZATION OF A PACLITAXEL MACROMOLECULAR PRODRUG

2004

Paclitaxel was attached to poly(hydroxyethylaspartamide) via a succinic spacer arm by a two-step protocol: (1) synthesis of 2'-O-succinyl-paclitaxel; (2) synthesis of PHEA-2'-O-succinyl-paclitaxel. The 2'-O-succinyl-paclitaxel derivative and the macromolecular conjugate were characterized by UV, IR, NMR and mass spectrometry analysis. The reaction yields were over 95% and the purity of products over 98%. Paclitaxel release and degradation from 2'-O-succinyl-paclitaxel occurred at a faster rate at pH 5.5 than 7.4. After 30 h of incubation at pH 5.5 and 7.4 the released free paclitaxel was about 40 and 20%, respectively. In plasma both drug release and degradation were found to occur at a hig…

MaleChemical PhenomenaPaclitaxelMacromolecular SubstancesPharmaceutical Sciencechemistry.chemical_compoundMicePharmacokineticsIn vivoCell Line TumorOrganic chemistryAnimalsProdrugschemistry.chemical_classificationMice Inbred BALB CChromatographyBioconjugationChemistryChemistry PhysicalMacromolecular SubstancesBiological activityGeneral MedicineEnzymePaclitaxelPolymeric prodrug Polymer therapeutics Conjugation αβ-Poly(N-2-hydroxyethyl)-dl-aspartamide PaclitaxelSettore CHIM/09 - Farmaceutico Tecnologico ApplicativoDrug Screening Assays AntitumorBiotechnologyConjugate
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N-Valproyl-L-Phenylalanine as new potential antiepileptic drug: Synthesis, characterization and in vitro studies on stability, toxicity and anticonvu…

2013

Valproic acid (VPA) is considered first-line drug in treatment of generalized idiopathic seizures such as absence, generalized tonic-clonic and myoclonic seizures. Among major antiepileptic drugs, VPA is also considered effective in childhood epilepsies and infantile spasms. Due to its broad activity, VPA acts as a mood stabilizer in bipolar disorder and it is useful in migraine prophylaxis. Despite its long-standing usage, severe reactions to VPA, such as liver toxicity and teratogenicity, are reported. To circumvent side effects due to structural characteristics of VPA, we synthesized in good yield a new VPA-aminoacid conjugate, the N-valproyl-L-Phenylalanine, and characterized by FT-IR, …

MaleDrugCell Membrane PermeabilityAminoacidic derivative Astrocytes toxicity CNS-Targeting Enzymatic Stability Hippocampal epilepsy Valproic acid.Cell Survivalmedicine.drug_classPhenylalaninemedicine.medical_treatmentmedia_common.quotation_subjectPrimary Cell CulturePhenylalaninePharmacologySettore BIO/09 - FisiologiaHippocampusTissue Culture TechniquesDrug StabilityDrug DiscoverymedicineAnimalsRats WistarEvoked Potentialsmedia_commonValproic AcidChemistryHydrolysisValproic AcidBiological TransportMood stabilizerMicrotomyHydrogen-Ion ConcentrationIn vitroRatsAnticonvulsantSettore CHIM/09 - Farmaceutico Tecnologico ApplicativoAstrocytesToxicityAnticonvulsantslipids (amino acids peptides and proteins)Conjugatemedicine.drug
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Acetaldehyde self-administration by a two-bottle choice paradigm: Consequences on emotional reactivity, spatial learning, and memory

2015

Abstract Acetaldehyde, the first alcohol metabolite, is responsible for many pharmacological effects that are not clearly distinguishable from those exerted by its parent compound. It alters motor performance, induces reinforced learning and motivated behavior, and produces different reactions according to the route of administration and the relative accumulation in the brain or in the periphery. The effective activity of oral acetaldehyde represents an unresolved field of inquiry that deserves further investigation. Thus, this study explores the acquisition and maintenance of acetaldehyde drinking behavior in adult male rats, employing a two-bottle choice paradigm for water and acetaldehyd…

MaleHealth (social science)MetaboliteEmotionsWistarSpatial LearningMorris water navigation taskSelf AdministrationAlcoholAcetaldehydeMotor ActivityToxicologyChoice BehaviorBiochemistryDevelopmental psychologyBehavioral Neurosciencechemistry.chemical_compoundMemoryAnimalsSpatial learning and memoryRats WistarMaze LearningMedicine (all)Cognitive flexibilityAcetaldehydeBrainAnxiety-like behaviorCognitionGeneral MedicineRatsAcetaldehyde in the brain; Anxiety-like behavior; Emotional reactivity; Spatial learning and memory; Two-bottle choice paradigm; Acetaldehyde; Animals; Brain; Choice Behavior; Emotions; Male; Maze Learning; Memory; Motor Activity; Rats; Rats Wistar; Self Administration; Spatial LearningTwo-bottle choice paradigmNeurologychemistryAnxiogenicEmotional reactivitySettore CHIM/09 - Farmaceutico Tecnologico ApplicativoAcetaldehyde in the brainSettore BIO/14 - FarmacologiaSelf-administrationPsychologyNeuroscienceAlcohol
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MATRICES OF A HYDROPHOBICALLY FUNCTIONALIZED HYALURONIC ACID DERIVATIVE FOR THE LOCOREGIONAL TUMOUR TREATMENT

2015

A hyaluronic acid (HA) derivative bearing octadecylamine and acylhydrazine functionalities has been here employed for the production of a paclitaxel delivering matrix for locoregional chemotherapy. Through a strategy consisting in a powder compression and a plasticization with a mixture water/ethanol, a physically assembled biomaterial, stable in solutions with physiologic ionic strengths, has been produced. Two different drug loading strategies have been adopted, by using paclitaxel as chemotherapic agent, and obtained samples have been assayed in terms of release in enhanced solubility conditions and in vitro and in vivo tumoural cytotoxicity. In particular sample with the best releasing …

MaleMaterials sciencePaclitaxelBiomedical EngineeringMice NudeBiocompatible MaterialsBiochemistryPaclitaxel release matrices hyaluronic acidBiomaterialschemistry.chemical_compoundMiceSubcutaneous TissueIn vivoNeoplasmsHyaluronic acidAnimalsHumansSolubilityHyaluronic AcidCytotoxicityMolecular BiologyCell DeathHydrolysisBody WeightOsmolar ConcentrationAcylhydrazineBiomaterialGeneral MedicineHCT116 CellsImmunohistochemistryXenograft Model Antitumor AssaysIn vitroPaclitaxelchemistrySettore CHIM/09 - Farmaceutico Tecnologico ApplicativoHydrophobic and Hydrophilic InteractionsBiotechnologyBiomedical engineeringNuclear chemistry
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Polymeric Nanocarriers for Magnetic Targeted Drug Delivery: Preparation, Characterization, and in Vitro and in Vivo Evaluation

2013

In this paper the preparation of magnetic nano- carriers (MNCs), containing superparamagnetic domains, is reported, useful as potential magnetically targeted drug delivery systems. The preparation of MNCs was performed by using the PHEA-IB-p(BMA) graft copolymer as coating material through the homogenization−solvent evaporation method. Magnetic and nonmagnetic nanocarriers containing flutamide (FLU-MNCs) were prepared. The prepared nanocarriers have been exhaustively characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), and magnetic measurements. Biological evaluation was performed by in vitro cytotoxicity and cell uptake tests and in vivo biodistribution …

MaleMaterials sciencePharmaceutical ScienceAntineoplastic AgentsNanotechnologyMagneticsDrug Delivery SystemsDynamic light scatteringIn vivoCell Line TumorDrug DiscoveryLNCaPAnimalsHumansDistribution (pharmacology)Tissue DistributionParticle SizeRats WistarMagnetite NanoparticlesDrug Carriersequipment and suppliesmagnetic nanocarrier magnetic targeting flutamide superparamagnetic nanoparticlesFlutamideIn vitroRatsTargeted drug deliverySettore CHIM/09 - Farmaceutico Tecnologico ApplicativoBiophysicsMolecular MedicineNanocarriersPeptideshuman activitiesSuperparamagnetismMolecular Pharmaceutics
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BRAIN-TARGETED SOLID LIPID NANOPARTICLES CONTAINING RILUZOLE: PREPARATION, CHARACTERIZATION AND BIODISTRIBUTION

2009

Aim: Developments within nanomedicine have revealed a great potential for drug delivery to the brain. In this study nanoparticulate systems as drug carriers for riluzole, with sufficiently high loading capacity and small particle size, were prepared to a reach therapeutic drug level in the brain. Materials & method: Solid lipid nanoparticles containing riluzole have great potential as drug-delivery systems for amyotrophic lateral sclerosis and were produced by using the warm oil-in-water microemulsion technique. The resulting systems obtained were approximately 88 nm in size and negatively charged. Drug-release profiles demonstrated that a drug release was dependent on medium pH. Biodi…

Maleamyotrophic lateral sclerosisBiodistributionMaterials scienceSOLID LIPID NANOPARTICLES BRAIN TARGETING RILUZOLEBiomedical EngineeringDrug delivery to the brainMedicine (miscellaneous)NanoparticleBioengineeringDevelopmentPharmacologyRats Sprague-DawleyPlasmaDrug StabilitySolid lipid nanoparticlemedicineAnimalsHumansGeneral Materials ScienceParticle Sizebiodistributionmodified releaseDrug CarriersRiluzoleBrainLipidsRiluzoleRatssolid lipid nanoparticlesNeuroprotective AgentsSettore CHIM/09 - Farmaceutico Tecnologico ApplicativoNanomedicineNanoparticlesParticle sizeDrug carriermedicine.drug
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Inhibitory effects of N-valproyl-L-tryptophan on high potassium, low calcium and low magnesium-induced CA1 hippocampal epileptiform bursting activity…

2012

N-valproyl-l-tryptophan (VPA-Tryp), new antiepileptic drug, was tested on CA1 hippocampal epileptiform bursting activity obtained by increasing potassium and lowering calcium and magnesium concentrations in the fluid perfusing rat brain slices. Each slice was treated with a single concentration (0.2, 0.5, 1 or 2 mM) of Valproate (VPA) or VPA-Tryp. Both burst duration and interburst frequency during and after treatment were off-line compared with baseline values. For both parameters, the latency and the length of statistically significant response periods as well as the magnitude of drug-induced responses were calculated. VPA-Tryp evoked fewer and weaker early excitatory effects than VPA on …

Maleantiepileptic drug valproic acidPotassiumchemistry.chemical_elementAction PotentialsCalciumHippocampal formationPharmacologyIn Vitro TechniquesInhibitory postsynaptic potentialSettore BIO/09 - Fisiologiaamino-acidic derivativeBurstingmedicineReaction Timehippocampal epilepsyAnimalsDrug InteractionsMagnesiumRats WistarCA1 Region HippocampalBiological PsychiatryValproic AcidAnalysis of VarianceDose-Response Relationship DrugMagnesiumDipeptidesElectric StimulationRatsPsychiatry and Mental healthNeurologychemistrySettore CHIM/09 - Farmaceutico Tecnologico ApplicativoExcitatory postsynaptic potentialPotassiuminterictal burstslipids (amino acids peptides and proteins)AnticonvulsantsNeurology (clinical)medicine.drugJournal of neural transmission (Vienna, Austria : 1996)
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N-valproyl-L-tryptophan for CNS-targeting: synthesis, characterization and efficacy in vitro studies of a new potential antiepileptic drug.

2010

A new aminoacidic derivative of valproic acid (VPA) has been synthesized and characterized by analytical and spectral data. The rationale for the preparation of such potential antiepileptic agent is based on the observation that chemical combination of the anticonvulsant pharmacophore, VPA with essential aminoacids could afford more effective and less toxic actives. The synthesis, characterization, physico-chemical parameters functional for crossing Blood Brain Barrier of N-valproyl-L-tryptophan (4) are reported. The Log D pH7.4 (0.3) indicates that (4) is adequate to cross biological membranes. Its chemical and enzymatic stability were assessed. The experiments indicate high stability of c…

Malemedicine.medical_treatmentHippocampal formationPharmacologyIn Vitro TechniquesBlood–brain barrierSettore BIO/09 - FisiologiaHippocampuschemistry.chemical_compoundDrug StabilityIn vivoDrug DiscoverymedicineAnimalsRats WistarValproic AcidEpilepsyDipeptidesAminoacidic derivative Antiepileptic Drug CNS-Targeting Enzymatic Stability Seizure Like Events Model Valproic acidIn vitroElectrophysiological PhenomenaRatsmedicine.anatomical_structureAnticonvulsantchemistrySettore CHIM/09 - Farmaceutico Tecnologico ApplicativoAnticonvulsantsPharmacophoreDerivative (chemistry)medicine.drugMedicinal chemistry (Shariqah (United Arab Emirates))
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Synthesis and evaluation of thermo-rheological behaviour and ionotropic crosslinking of new gellan gum-alkyl derivatives.

2018

Abstract This paper reports the synthesis and the physicochemical characterization of two series of gellan gum (GG) derivatives functionalized with alkyl chains with different number of carbon, from 8 to 18. In particular, low molecular weight gellan gum samples with 52.6 or 96.7 kDa, respectively, were functionalized with octylamine (C8), dodecylamine (C12) and octadecylamine (C18) by using bis(4-nitrophenyl) carbonate (4-NPBC) as a coupling agent. Thermo-rheological and ionotropic crosslinking properties of these gellan gum-alkyl derivatives were evaluated and related to the degree of derivatization in alkyl chains. Results suggested as length and degree of derivatization differently infl…

Materials Chemistry2506 Metals and AlloysPolymers and Plastics02 engineering and technologymacromolecular substances010402 general chemistry01 natural sciencesAlkylaminesGellan gumAlkylaminechemistry.chemical_compoundRheologyPolymer chemistryMaterials ChemistryDerivatizationAlkylchemistry.chemical_classificationPolymers and PlasticOrganic ChemistryHydrogels021001 nanoscience & nanotechnologyGellan gum0104 chemical sciencesHydrogelchemistrySettore CHIM/09 - Farmaceutico Tecnologico ApplicativoSelf-healing hydrogels0210 nano-technologyRheologyIonotropic effectCarbohydrate polymers
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