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Human Papillomavirus Type 16 E7 Peptide-Directed CD8+ T Cells from Patients with Cervical Cancer Are Cross-Reactive with the Coronavirus NS2 Protein

2003

ABSTRACTHuman papillomavirus type 16 (HPV16) E6 and E7 oncoproteins are required for cellular transformation and represent candidate targets for HPV-specific and major histocompatibility complex class I-restricted CD8+-T-cell responses in patients with cervical cancer. Recent evidence suggests that cross-reactivity represents the inherent nature of the T-cell repertoire. We identified HLA-A2 binding HPV16 E7 variant peptides from human, bacterial, or viral origin which are able to drive CD8+-T-cell responses directed against wild-type HPV16 E7 amino acid 11 to 19/20 (E711-19/20) epitope YMLDLQPET(T) in vitro. CD8+T cells reacting to the HLA-A2-presented peptide from HPV16 E711-19(20)recogni…

Evaluation of HBs, HBc, and frCP virus-like particles for expression of human papillomavirus 16 E7 oncoprotein epitopes.

2002

<i>Objectives:</i> In an attempt to develop virus-like particles (VLPs) as experimental vaccine against human papilloma virus (HPV)-induced tumours, the HPV16 E7 oncoprotein epitopes spanning amino acid (aa) residues 35–98 were expressed on three proteins capable of VLP formation: hepatitis B virus (HBV) surface (HBs) and core (HBc) antigens, and RNA phage fr coats (frCP). <i>Methods:</i> The profile of immunoglobulin isotypes induced in Balb/C mice after immunization with purified chimeric proteins was studied. <i>Results:</i> The HBs*-E7(35–54) protein expressing E7 residues 35–54 between residues 139 and 142 of the HBs carrier formed HBs-like particles…

SARS-CoV-2 envelope protein topology in eukaryotic membranes

2020

Coronavirus E protein is a small membrane protein found in the virus envelope. Different coronavirus E proteins share striking biochemical and functional similarities, but sequence conservation is limited. In this report, we studied the E protein topology from the new SARS-CoV-2 virus both in microsomal membranes and in mammalian cells. Experimental data reveal that E protein is a single-spanning membrane protein with the N-terminus being translocated across the membrane, while the C-terminus is exposed to the cytoplasmic side (Nt lum /Ct cyt ). The defined membrane protein topology of SARS-CoV-2 E protein may provide a useful framework to understand its interaction with other viral and ho…

Tumor targeting of baculovirus displaying a lymphatic homing peptide.

2008

Background Tumor-associated cells and vasculature express attractive molecular markers for site-specific vector targeting. To attain tumor-selective tropism, we recently developed a baculovirus vector displaying the lymphatic homing peptide LyP-1, originally identified by ex vivo/in vivo screening of phage display libraries, on the viral envelope by fusion to the transmembrane anchor of vesicular stomatitis virus G-protein. Methods In the present study, we explored the specificity and kinetics of viral binding and internalization as well as in vivo tumor homing of the LyP-1 displaying virus to elucidate the applicability of baculovirus for targeted therapies. Results We demonstrated that th…

Fractional Hardy inequalities and visibility of the boundary

2013

We prove fractional order Hardy inequalities on open sets under a combined fatness and visibility condition on the boundary. We demonstrate by counterexamples that fatness conditions alone are not sufficient for such Hardy inequalities to hold. In addition, we give a short exposition of various fatness conditions related to our main result, and apply fractional Hardy inequalities in connection to the boundedness of extension operators for fractional Sobolev spaces.

Pēcoperācijas perioda izvērtējums pēc radzenes transplantācijas vispārējā anestēzijā

2020

Bakalaura darba tēma ir “Pēcoperācijas perioda izvērtējums pēc radzenes transplantācijas vispārējā anestēzijā”. Darba tēmas aktualitāte ir tajā, ka radzenes transplantācijas operācijas visā pasaulē un Latvijā tiek veiktas arvien vairāk, tāpēc ir būtiski novērtēt pēcoperācijas periodu pēc šīm operācijām, lai izvērtēt aprūpes procesu, pacientu sūdzības un dokumentācijas kvalitāti un nepieciešamības gadījumā veikt uzlabojums. Darba mērķis ir izvērtēt pēcoperācijas periodu pacientam pēc radzenes transplantācijas operācijas vispārējā anestēzijā. Bakalaura darba mērķa saniegšanai autore izvirzija sekojošus darba uzdevumus - apkopot un analizēt zinātnisko literatūru par radzenes transplantāciju un…

Editorial : Visual mismatch negativity (vMMN) : A unique tool in investigating automatic processing

2022

The impact of Mt. Etna volcanic emissions on the atmospheric deposition: developments and improvements during three decades of studies

2022

Volcanic emissions are an important source of gases and particles to the atmosphere. Mount Etna discharges a permanent volcanic plume consisting of water vapor and gaseous species (CO2, SO2, HCl and HF, mainly), metals, acid droplets, and solid particles. This plume interacts with meteoric water during precipitations events, so the chemical signature of the plume is impressed on the rainwater. Over the past 32 years, the impact of Etna's emissions on atmospheric deposition has been investigated in many research projects by our team. Sampling methodologies and analytical procedures have been improved over the years. The first study of the concentration of water-soluble inorganic ions in week…

Decoupled control scheme of grid-connected split-source inverters

2017

Grid-connected power conversion systems for renewable energy sources must fulfill several requirements, e.g., the high efficiency, the reduced cost and complexity, and, quite often, the boost capabilities that is usually achieved using a front-end dc–dc boost converter before the inversion stage, leading to a two-stage architecture. Meanwhile, single-stage power conversion systems, which perform the boosting operation within the inversion one, offer some potential advantages, in terms of reducing the complexity and the volume of the whole system. Among several proposed options, the split-source inverter (SSI) has been recently proposed by Abdelhakim et al. as an alternative option with some…

Relationship between volume and energy of vector fields

2001

Abstract A unified study of energy and volume functionals is presented here by determining the critical points of a functional that extends simultaneously energy and volume and that is defined on the product of the manifold of smooth maps C∞(M,N) times the manifold M of riemannian metrics on M. The restriction of this functional to different submanifolds of the space of vector fields X (M)× M is also considered, and used to study several functionals generalizing volume and energy or total bending of vector fields