Search results for "TPA"

P-992 - Smoking during pregnancy and postnatal period and postpartum depression

2012

Introduction Substance use in pregnancy is an increasingly common problem and has become an important public health issue. Postpartum depression has a high prevalence (10%) of women in Spanish population. Objetive/aim To study the impact of perinatal tobacco use in postpartum depression. Methods A cohort study of 1804 puerperal Spanish Caucasian women of general population. Variables collected: socio-demographic, obstetric, personal and family psychiatric history, substance use during pregnancy and 6 months postpartum, depressive symptoms (EPDS) and anxiety traits (STAI) at 2–3 days, 8 weeks and 32 weeks postpartum. Major postpartum depression (MPD) (EPDS>9) were confirmed through a structu…

Tranexamic acid for the prevention of blood loss after cesarean section: an updated systematic review and meta-analysis of randomized controlled tria…

2023

Objective: Tranexamic acid (TXA) is a cost-effective intervention for the prevention of postpartum hemorrhage (PPH) in women undergoing cesarean section but the evidence to support its use is conflicting. We conducted this meta-analysis to evaluate the efficacy and safety of TXA in low- and high-risk cesarean deliveries. Data sources: We searched MEDLINE (via PubMed), Embase, the Cochrane Library, ClinicalTrials.gov, and WHO International Clinical Trials Registry Platform (ICTRP) portal from inception to April 2022 (updated October 2022 and February 2023) with no language restrictions. Additionally, grey literature sources were also explored. Study eligibility criteria: All randomized contr…

Postpartum Hemorrhage: Conservative Treatments

2023

Postpartum hemorrhage (PPH) is an obstetric emergency representing the first cause of obstetric mortality and a frequent cause of severe maternal morbidity. It can complicate vaginal or cesarean deliveries and accounts for 25% of all maternal deaths worldwide, as reported by the World Health Organization (WHO). Primary PPH is defined as blood loss from the genital tract of at least 500 ml after vaginal or 1000 ml following cesarean delivery within 24 h postpartum, whereas secondary PPH is defined as any significant bleeding from the birth canal occurring between 24 h and 12 weeks postnatally. Uterine atony is reported as the main cause of PPH and accounts for 75%–90% of primary PPH. When ut…

Parental Pre- and Postpartum Mental Health Predicts Child Mental Health and Development

2017

Objective To identify interplay of early maternal and paternal mental health symptoms for predicting child mental health and development. Background Research on family mental health has largely excluded fathers, although the well-being of both parents is likely to be important for child development. In this study, we analyzed (a) intrafamilial dynamics between mothers' and fathers' early mental health symptoms and (b) the importance of separate (mother and father) and joint (additive, hierarchical, and buffering) theoretical models of parental mental health for predicting child mental health and development. Method Finnish mothers and fathers (N = 763), half of whom conceived through assist…

2014

Background: The aim of this study was to explore how pelvic girdle pain after delivery influences women’s daily life in Norway. Knowledge about living with post-partum pelvic girdle pain is lacking. Method: A phenomenological–hermeneutical design with qualitative semi-structured interviews was used. A strategic selection procedure was chosen to recruit participants from physiotherapy clinics and a regional hospital in Norway. Five women with clinically verified pelvic girdle pain after delivery were included. Data were imported into NVivo9 and analysed in three steps: naive reading, structural analysis and comprehensive understanding of the text. Results: Three themes influencing the women’…

No evidence of ATP1A2 involvement in 12 multiplex Italian families with benign familial infantile seizures

2005

A missense mutation in the gene encoding the alpha(2) Subunit of the Na+,K+ ATPase pump (ATP1A2) was found in a family with both familial hemiplegic migraine (FHM) and Benign Familial Infantile Seizures (BFIC). As it is still unclear whether ATP1A2 is responsible for pure BFIC syndromes, we checked mutations of the ATP1A2 gene in probands of 12 Italian multiplex families with pure BFIC, who were negative for mutations in the SCN2A gene. We screened the ATP1A2 gene by denaturing high performance liquid chromatography (D-HPLC) and direct sequencing of DNA fragments showing an aberrant elution pattern. We found one exonic variant and five intronic variants, none leading to significant amino ac…

ATP1A2 mutations in 11 families with familial hemiplegic migraine.

2005

Abstract Familial hemiplegic migraine (FHM) is an autosomal dominant form of migraine with aura. The disease is caused by mutations of at least three genes among which two have been identified, CACNA1A and ATP1A2. Very few mutations have been identified so far in ATP1A2. We screened the coding sequence of ATP1A2 in 26 unrelated FHM probands in whom CACNA1A screening was negative. A total of eight different mutations were identified in 11 of the probands (41%), including six missense mutations, one small deletion leading to a frameshift, and one in frame deletion. All were novel mutations. Two mutations were recurrent, in three and two families, respectively. Genotyping of 94 relatives of th…

Mode of cell death induction by pharmacological Vacuolar H+-ATPase (V-ATPase) inhibition.

2012

The vacuolar H+-ATPase (V-ATPase), a multisubunit proton pump, has come into focus as an attractive target in cancer invasion. However, little is known about the role of V-ATPase in cell death, and especially the underlying mechanisms remain mostly unknown. We used the myxobacterial macrolide archazolid B, a potent inhibitor of the V-ATPase, as an experimental drug as well as a chemical tool to decipher V-ATPase-related cell death signaling. We found that archazolid induced apoptosis in highly invasive tumor cells at nanomolar concentrations which was executed by the mitochondrial pathway. Prior to apoptosis induction archazolid led to the activation of a cellular stress response including …

The Role of Low Complexity Regions in Protein Interaction Modes: An Illustration in Huntingtin

2021

Low complexity regions (LCRs) are very frequent in protein sequences, generally having a lower propensity to form structured domains and tending to be much less evolutionarily conserved than globular domains. Their higher abundance in eukaryotes and in species with more cellular types agrees with a growing number of reports on their function in protein interactions regulated by post-translational modifications. LCRs facilitate the increase of regulatory and network complexity required with the emergence of organisms with more complex tissue distribution and development. Although the low conservation and structural flexibility of LCRs complicate their study, evolutionary studies of proteins …

Structural analysis of Borrelia burgdorferi periplasmic lipoprotein BB0365 involved in Lyme disease infection.

2019

The periplasmic lipoprotein BB0365 of the Lyme disease agent Borrelia burgdorferi is expressed throughout mammalian infection and is essential for all phases of Lyme disease infection; its function, however, remains unknown. In the current study, our structural analysis of BB0365 revealed the same structural fold as that found in the NqrC and RnfG subunits of the NADH:quinone and ferredoxin:NAD+ sodium-translocating oxidoreductase complexes, which points to a potential role for BB0365 as a component of the sodium pump. Additionally, BB0365 coordinated Zn2+ by the His51, His55, His140 residues, and the Zn2+ -binding site indicates that BB0365 could act as a potential metalloenzyme; therefore…