Search results for "TPA"

Polar/Ionizable Residues in Transmembrane Segments: Effects on Helix-Helix Packing

2012

The vast majority of membrane proteins are anchored to biological membranes through hydrophobic alpha-helices. Sequence analysis of high-resolution membrane protein structures show that ionizable amino acid residues are present in transmembrane (TM) helices, often with a functional and/or structural role. Here, using as scaffold the hydrophobic TM domain of the model membrane protein glycophorin A (GpA), we address the consequences of replacing specific residues by ionizable amino acids on TM helix insertion and packing, both in detergent micelles and in biological membranes. Our findings demonstrate that ionizable residues are stably inserted in hydrophobic environments, and tolerated in t…

Phylogenetic profiling and cellular analyses of ARL16 reveal roles in traffic of IFT140 and INPP5E

2021

ABSTRACTThe ARF family of regulatory GTPases is ancient, with 16 members predicted to have been present in the last eukaryotic common ancestor. Our phylogenetic profiling of paralogs in diverse species identified four family members whose presence correlates with that of a cilium/flagellum: ARL3, ARL6, ARL13, and ARL16. No prior evidence links ARL16 to cilia or other cell functions, despite its presence throughout eukaryotes. Deletion of ARL16 in MEFs results in decreased ciliogenesis yet increased ciliary length. We also found Arl16 KO in MEFs to alter ciliary protein content, including loss of ARL13B, ARL3, INPP5E, and the IFT-A core component IFT140. Instead, both INPP5E and IFT140 accum…

Effects of sulindac sulfide on the membrane architecture and the activity of gamma-secretase.

2007

gamma-Secretase is a membrane-embedded multi-protein complex that catalyzes the final cut of the Alzheimer's disease-related amyloid precursor protein (APP) to amyloid-beta peptides of variable length (37-43 amino acids) via an unusual intramembrane cleavage. Recent findings propose that some commonly used non-steroidal anti-inflammatory drugs (NSAIDs) have the ability to modulate specifically gamma-secretase activity without inhibiting the enzyme as a whole. These drugs may shift the processing of APP from the longer amyloid-beta 42 peptide towards shorter, less fibrillogenic and less toxic amyloid-beta species. We hypothesize that gamma-secretase activity, as an enzyme that is strictly as…

A replication study for the prediction of doxepine-response in depressed outpatients.

1988

Le funzioni metacognitive nei pazienti con disturbi del comportamento alimentare. Uno studio sul trattamento di gruppo psicodinamico

2010

There is an emerging empirical evidence that patients with eating disorders have severe metacognitive concerns, i.e. ability to reflect on mental states. This single-case study aims to explore the relationship between limited metacognition and eating symptoms in six patients who attended a long-term group treatment. This study also aims at analysing the change of patients metacognition over the course of treatment. All the patients were female, with a mean age of 17 years. Three patients have a diagnosis of anorexia nervosa, and three have a diagnosis of bulimia nervosa. The group treatment was delivered in a outpatient clinic of the hospital of Acireale (CT). The SVaM (Carcione et al., 199…

Cumpleaños feliz

1997

Subjective symptomatology of asthma: validation of the asthma symptom checklist in an outpatient Spanish population.

1997

The objective of this study was to validate the Asthma Symptom Checklist (ASC) so that it could be reliably used to assess the subjective symptomatology of asthma attacks in our context. Subjective symptomatology of asthma was examined in a group of 100 adult Spanish outpatients (57 women, 43 men; 17-69 years of age) with asthma. All of them completed the modified version of the ASC as well as questionnaires of depression, anxiety, and self-management of asthma (self-efficacy expectancies and health care utilization). Data about duration and severity of asthma, as well as dyspnea and %FEV1, were also recorded. The highest reliability Cronbach alpha indexes were for the panic-fear and fatigu…

The impact of SARS-COV2 pandemic on the management oF IPF patients: Our narrative experience

2021

Abstract Background The SARS-CoV-2 pandemic has changed the health-care systems around the world in a remarkable way. We describe the strategies adopted to cope with the limitations imposed by the pandemic to the access to health care by patients diagnosed with idiopathic Pulmonary Fibrosis (IPF). Material and methods We conducted a retrospective observational analysis including IPF patients under antifibrotic drugs (nintedanib and pirfenidone) that accessed to the Outpatient clinic of the University of Palermo, Italy. Patients received a phone number and an email address in case of any urgency and a virtual meeting was settled up monthly. Results 40 patients (M/F: 30/10) were followed up, …

Cercospora beticola toxins. Part XVII. The role of the beticolin/Mg2+ complexes in their biological activity Study of plasma membrane H+-ATPase, vacu…

1996

Beticolin-1 and beticolin-2, yellow toxins produced by the phytopathogenic fungus Cercospora beticola, inhibit the plasma membrane H(+)-ATPase. Firstly, since beticolins are able to form complexes with Mg2+, the role of the beticolin/Mg2+ complexes in the inhibition of the plasma membrane proton pump has been investigated. Calculations indicate that beticolins could exist under several forms, in the H(+)-ATPase assay mixture, both free or complexed with Mg2+. However, the percentage inhibition of the H(+)-ATPase activity is correlated to the concentration of one single form of beticolin, the dimeric neutral complex Mg2H2B2, which appears to be the active form involved in the H(+)-ATPase inh…

Darbinieku kontroles sistēma, izmantojot biometrijas datus

2019

Kvalifikācijas darba mērķis ir izstrādāt darbinieku kontroles sistēmu, kura izmanto biometrijas datus (sejas atpazīšanu), un ar kuras palīdzību var koordinēt un pārvaldīt firmas darbinieku darbības un to personīgos datus, un tas ļauj efektīvāk organizēt uzņēmuma darbību. Šī sistēma ir izveidota C++ programmēšanas valodā izmantojot QT Creator ietvaru un atvērtā koda bibliotēku OpenCv. Sistēmas dati tiek glabāti SQLite datubāzē. Sistēma tika veidota izmantojot gan ūdenskrituma (waterfall) modeli, gan spējas (agile) izstrādes metodes. Sistēma sastāv no 4 moduļiem - autentificēšanās, lietotāja, atpazīšanas, atskaišu.