Search results for "TRES"
showing 10 items of 6927 documents
Functional exploration of the stress response in microorganisms of technological interest : dynamics of membrane response after an ethanol shock in S…
2015
The study of membrane response induced by ethanol stress in Saccharomyces cerevisiae aims to understand mechanisms involved in the survival of eukaryotic cells submitted to high ethanol concentrations. The cell membrane by its position between the intra- and extracellular environment is the first target of ethanolic perturbations. Experiments performed in this study aimed to characterize the maintain of the functional integrity of the membrane in relation to the sterol composition in the yeast S. cerevisiae submitted to different types of ethanolic disturbances: increasing concentrations of ethanol in the growth medium; ethanolic shocks of increasing magnitude; shock 20% ethanol for 15 minu…
Osmotic stress affects the stability of freeze-dried Lactobacillus buchneri R1102 as a result of intracellular betaine accumulation and membrane char…
2014
Aims To help cells to better resist the stressful conditions associated with the freeze-drying process during starter production, we investigated the effect of various osmotic conditions on growth, survival and acidification activity of Lactobacillus buchneri R1102, after freeze-drying and during storage for 3 months at 25°C. Methods and Results High survival rates during freeze-drying, but not during storage, were obtained when 0·1 mol l−1 KCl was added at the beginning of fermentation, without any change in membrane properties and betaine accumulation. This condition made it possible to maintain a high acidification rate throughout the process. In contrast, the addition of 0·6 mol l−1 KCl…
The Crosstalk of Adipose-Derived Stem Cells (ADSC), Oxidative Stress, and Inflammation in Protective and Adaptive Responses
2020
International audience; The potential use of stem cell-based therapies for the repair and regeneration of various tissues and organs is a major goal in repair medicine. Stem cells are classified by their potential to differentiate into functional cells. Compared with other sources, adipose-derived stem cells (ADSCs) have the advantage of being abundant and easy to obtain. ADSCs are considered to be tools for replacing, repairing, and regenerating dead or damaged cells. The capacity of ADSCs to maintain their properties depends on the balance of complex signals in their microenvironment. Their properties and the associated outcomes are in part regulated by reactive oxygen species, which medi…
Participation of nutritional and environmental factors in aging of the retina and age-related retinopathies
2012
With advanced age, aging of the human retina can evolve into pathological forms, age-related macular degeneration (AMD) or diabetic retinopathy (DR). Meanwhile, epidemiological studies suggest that a diet rich in long-chain omega-3 polyunsaturated fatty acids (LC-PUFA) such as EPA and DHA, potentially protects against the development of AMD and insulin resistance, the main risk factor for DR. Within this context, our research objectives were to assess: 1 - the impact of endogen and environmental factors, known to trigger oxidative stress, advanced glycation end-products (AGEs) or insulin resistance, on the function and aging of the retina, and 2 - the response of the retina to a omega-3 LC-…
Cardiotoxicity of cancer therapy
2011
Despite the development of new anti-cancer drugs such as anti-tyrosine kinases or anti-angiogenic therapy, cancer mortality remains high. These new molecules associated with advances in cancer surgery, radiotherapy and chemotherapy have succeeded in improving life expectancy in these patients. It has also allowed a long-term evaluation of the cardiovascular impact of these therapies. Many chemotherapy drugs, such as anthracyclines, lead to impaired cardiac function. The pathophysiological mechanisms of this cardiac dysfunction are complex, intricate and remain partially unknown. To reduce this cardiotoxicity, different pathways concerning administration modalities, drug presentation and the…
Monounsaturated fatly acid metabolism and colon cancer cells proliferation : role of Stearoyl-CoA desaturase-1 and effect of conjugated linoleic acid…
2012
Cancer cells adapt their metabolism in response to signals from the microenvironment and proliferation. Thus, MonoUnsaturated Fatty Acid (MUFA) synthesis is increased in colon cancer cells, and associated with increased Stearoyl-CoA Desaturase (SCD) activity, the rate limiting enzyme of MUFA biosynthesis. Polyunsaturated Fatty Acid (PUFA), as isomers of conjugated linoleic acid (CLA), exert inhibitor activity on SCD-1 and have anti-cancer properties, but their mechanisms are not yet clear. In this context, the aim of this work was first to evaluate the role of SCD-1 in the proliferation of colon cancer cells (CCC) and to define the underlying mechanisms. In a second time, we provide new inf…
: J. Sci. Food Agric.
2017
Background: The measurement of carbon isotopic discrimination in grape sugars at harvest (δ13 C) is an integrated assessment of water status during ripening. It is an efficient alternative to assess variability in the field and discriminate between management zones in precision viticulture, but further work is needed to completely understand the signal.; Results: This work, spanning over 3 years, performed in a hillslope toposequence in Burgundy, delineates the relationships between main soil properties (gravel amount, slope, texture) and the grapevine water status assessed by δ13 C. The highest δ13 C, indicating most severe water deficit, was recorded in gravelly soils on steep slopes. The…
Processus écologiques et évolutifs influençant la colonisation de l'ambroisie à feuilles d'armoise (Ambrosia artemisiifolia l.) en France.
2012
Understanding of the mechanisms behind the success of the invasive species is essential to manage current biological invasions and to prevent the risks of the futures ones. Using a conceptual framework integrating ecological and evolutionary processes, this work aimed to analyse the factors of the common ragweed colonization in France. First of all, the study of biotic and abiotic interactions has shown the ability of common ragweed to tolerate herbivory and water stress. Common ragweed is able to buffer defoliation through an efficient compensatory growth with no consequence on the reproduction. Herbivory tolerance has been maintained in introduced populations even if herbivory pressure is…
Study and characterization of the "Viable but non-culturable" state in Saccharomyces cerevisiae
2013
The viable but not culturable (VBNC) state has been studied in detail in bacteria. Bycontrast the VBNC state in other microorganisms, including particularly eukaryotes, has receivedmuch less attention. However, it has been suggested that in wine, Brettanomyces yeast cells mayenter a Viable But Not Culturable State, in particular in the presence of high, sulfur dioxide(SO2) concentration.To provide conclusive evidences for the existence of a VBNC state in yeast, especially in S.cerevisiae as a model organism, the capacity of different S cerevisiae strains to become viableand not cultivable after a sulfite stress with various concentrations of SO2 was studied by flowcytometry (FCM) using fluo…