Search results for "TRIF"

showing 10 items of 1419 documents

Enhanced oxidative susceptibility and reduced antioxidant content of metabolic precursors of small, dense low-density lipoproteins.

2001

Abstract PURPOSE: Elevated plasma concentrations of low-density lipoproteins (LDL) increase risk for coronary heart disease. However, lipoprotein profiles rich in small, dense LDL particles confer greater risk than those that mainly consist of large, buoyant LDL. This may be due, in part, to the greater oxidative susceptibility of small, dense LDL. In the current studies, we tested whether differences in the oxidative behavior of buoyant and dense LDL arise from differences in their immediate metabolic precursors, intermediate-density lipoproteins. SUBJECTS AND METHODS: We compared the properties of intermediate-density lipoproteins and buoyant and dense LDL subfractions in 9 subjects with …

AdultMalemedicine.medical_specialtyAntioxidantParinaric Acidmedicine.medical_treatmentOxidative phosphorylationAntioxidantsReference ValuesInternal medicineMedicineHumansPolyacrylamide gel electrophoresisFluorescent DyesIntermediate-density lipoproteinbusiness.industryGeneral MedicineLipoproteins LDLEndocrinologyPhenotypeFatty Acids Unsaturatedlipids (amino acids peptides and proteins)Electrophoresis Polyacrylamide GelFemaleUltracentrifugebusinessQuantitative analysis (chemistry)Oxidation-ReductionLipoproteinThe American journal of medicine
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Low-density lipoproteins generated during an oral fat load in mild hypertriglyceridemic and healthy subjects are smaller, denser, and have an increas…

2005

Triglyceride-rich lipoproteins generated during the postprandial phase are atherogenic. Large very low-density lipoproteins (LDLs) or chylomicrons (CMs) are not as atherogenic as their remnants (Rem). Small and dense LDLs are associated with cardiovascular disease. Low-density lipoprotein size is partly under genetic control and is considered as a relatively stable LDL feature. In this article, we present data on retinyl palmitate kinetics correlated with the modification of LDL features in terms of size, density, and in vitro receptor binding affinity after an oral fat load. Six nondiabetic, hypertriglyceridemic (HTG) patients and 6 healthy controls were examined. Low-density lipoprotein s…

AdultMalemedicine.medical_specialtyRetinyl EstersSettore MED/09 - Medicina InternaEndocrinology Diabetes and MetabolismBinding CompetitiveModels Biologicalchemistry.chemical_compoundEndocrinologyInternal medicineRetinyl palmitateCell Line TumorChylomicronsmedicineHumansReceptorVitamin AHypertriglyceridemiaLow-density lipoproteins hypertriglyceridemia Fasting and postprandial LDLsTriglycerideCatabolismChemistryFastingFibroblastsPostprandial PeriodDietary FatsLipidsLipoproteins LDLKineticsEndocrinologyPostprandialReceptors LDLlipids (amino acids peptides and proteins)Density gradient ultracentrifugationElectrophoresis Polyacrylamide GelFemaleDiterpenesUltracentrifugationLipoproteinChylomicronMetabolism: clinical and experimental
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Microcentrifuge or Automated Hematological Analyzer to Assess Hematocrit in Exercise? Effect on Plasma Volume Loss Calculations

2016

The assessment of plasma volume loss (∆PV) induced by exercise can be estimated from changes in hematocrit (Htc) and hemoglobin (Hb), and it is essential when investigating the metabolic or biologic response to exercise of circulating biomarkers. We aimed to ascertain whether the estimation of ∆PV may differ when Hb and Htc are determined by automated hematological analyzer (AHA) versus manual methods. Twenty-five healthy male subjects performed a maximal running incremental exercise. Blood samples were taken before exercise, immediately after exercise, and after a 30-min recovery. Hb and Htc (Htc-AHA) were determined by an AHA. Htc was also determined by microcentrifugation (Htc-M). The ∆P…

AdultMalemedicine.medical_specialtySpectrum analyzerSysmexAnalytical chemistryCentrifugationHematocritPlasma volumeIncremental exerciseHemoglobinsYoung Adult03 medical and health sciences0302 clinical medicineInternal medicinemedicineHumanshemoconcentrationhealth care economics and organizationsplasma volumeAutomation LaboratoryexerciseBiologic responsemedicine.diagnostic_testChemistryDill and Costill equation030229 sport sciencesMiddle AgedhemoglobinHemoconcentrationDill and Costill equation; Sysmex; exercise; hemoconcentration; hemoglobin; plasma volumeHealthy Volunteersdigestive system diseasesComputer Science ApplicationsMedical Laboratory TechnologyCirculating biomarkersHematocritCardiologyHemoglobinBiomarkers030215 immunologySLAS Technology
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Inhibition of thromboxane biosynthesis by triflusal in type 2 diabetes mellitus.

2004

Abstract Triflusal is an antiplatelet drug related to aspirin, with different pharmacological properties and a lower haemorrhagic risk. We aimed at comparing their effects on platelet and endothelial activation in type 2 diabetes mellitus (T2DM). In a randomized, double-blind, parallel group study, we compared the effects of three daily regimens (300, 600, and 900mg) of triflusal, and aspirin (100mg/day) on urinary 11-dehydro-thromboxane (TX)B 2 , index of in vivo platelet activation, ex vivo platelet function using the analyzer PFA-100, plasma von Willebrand factor (vWF), P-selectin, intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and serum nitrite a…

AdultMalemedicine.medical_specialtyThromboxaneaspirinRadioimmunoassaychemistry.chemical_compoundThromboxane A2Von Willebrand factorDouble-Blind MethodInternal medicinetype 2 diabetes triellitusvon Willebrand Factormedicineplatelet activationHumansPlateletPlatelet activationAgedRetrospective StudiesAspirinbiologyDose-Response Relationship DrugMiddle AgedSalicylatesThromboxane B2Thromboxane B2triflusalP-SelectinEndocrinologychemistryDiabetes Mellitus Type 2biology.proteinTriflusalFemaleCardiology and Cardiovascular MedicineBiomarkersPlatelet Aggregation Inhibitorsmedicine.drugFollow-Up Studies
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Distinct patterns of heparin affinity chromatography VLDL1 and VLDL2 subfractions in the different dyslipidaemias

2007

Very low density lipoprotein (VLDL) 1 and 2 were fractionated by heparin affinity chromatography into a bound and an unbound fraction and the different subfractions were quantified in 17 normolipidaemic (NL), 13 hypercholesterolaemic (HC), 10 hypertriglyceridaemic (HTG) and 11 combined hyperlipidaemic subjects (CHL). Unbound VLDL1 and VLDL2 were, respectively, 1.9- and 2.2-fold richer in triglycerides than bound VLDL1 and VLDL2. In HTG and CHL the concentration of all the VLDL subfractions was increased and plasma triglyceride level was correlated to unbound VLDL1 and to bound VLDL1 (respectively, r=0.86 (p<0.001) and r=0.77 (p<0.01) in HTG and r=0.73 (p<0.001) and r=0.62 (p<0.05) in CHL). …

AdultMalemedicine.medical_specialtyVery low-density lipoproteinHypercholesterolemiaHyperlipidemia Familial CombinedLipoproteins VLDLChromatography AffinityGlycosaminoglycanchemistry.chemical_compoundAffinity chromatographyInternal medicinemedicineHumansApolipoproteins BDyslipidemiasHypertriglyceridemiaTriglycerideHeparinCholesterolHypertriglyceridemiaAnticoagulantsHeparinMiddle Agedmedicine.diseaseEndocrinologychemistryFemaleCardiology and Cardiovascular MedicineUltracentrifugationProtein BindingLipoproteinmedicine.drugAtherosclerosis
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Closed vs. Open Oocyte Vitrification Methods Are Equally Effective for Blastocyst Embryo Transfers: Prospective Study from a Sibling Oocyte Donation …

2020

&lt;b&gt;&lt;i&gt;Purpose:&lt;/i&gt;&lt;/b&gt; To assess whether open and closed vitrification protocols are equally effective for sibling-oocyte cycles when performing blastocyst embryo transfers. &lt;b&gt;&lt;i&gt;Materials and Methods:&lt;/i&gt;&lt;/b&gt; A prospective study was set up comparing the open and the closed vitrification techniques in oocyte recipients sharing sibling oocytes between 2014 and 2016. Sibling oocytes were randomly and equally assigned into the closed group (oocytes vitrified in a closed system) or the open group (oocytes vitrified in an open system). Intracytoplasmic sperm injection was performed on all cases. Embryo transfers were performed on day 5. Power anal…

AdultPregnancy Ratemedicine.medical_treatmentIntracytoplasmic sperm injectionAndrology03 medical and health sciences0302 clinical medicineHuman fertilizationPregnancymedicineHumansVitrificationBlastocystAdult Birth RateEmbryo ImplantationProspective StudiesSperm Injections IntracytoplasmicBirth RateSurvival rateCryopreservation030219 obstetrics & reproductive medicineOocyte Donationbusiness.industryObstetrics and GynecologyEmbryoOocyteEmbryo TransferVitrificationInfertility; Oocyte donation; Vitrificationmedicine.anatomical_structureBlastocystReproductive Medicine030220 oncology & carcinogenesisInfertilityOocytesFemaleLive birthbusinessGynecologic and obstetric investigation
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Improving ovarian tissue cryopreservation for oncologic patients: slow freezing versus vitrification, effect of different procedures and devices.

2013

Objective To compare slow freezing (SF) with four vitrification techniques (VT) for cryopreservation of ovarian tissue (OT) and to evaluate the best protocol for human OT in a xenograft model. Design Experimental study. Setting University hospital. Patient(s) Patients undergoing fertility preservation. Animal(s) Ovariectomized nude mice. Intervention(s) Cryopreservation of bovine OT after SF and four VTs (VT1, VT2, VT3, and VT4) by combining two cryoprotectant vitrification solutions (VS1 and VS2) and two devices (metallic grid and ethyl vinyl acetate bag), after which the cryopreservation of human OT by SF and VT1 and xenograft into nude mice. Main Outcome Measure(s) Follicular densities, …

AdultTime FactorsCryoprotectantAdolescentPopulationMice NudeBreast NeoplasmsBiologyCryopreservationAndrologyMiceRandom AllocationYoung AdultFresh TissueFollicular phaseAnimalsHumansVitrificationOvarian tissue cryopreservationeducationCell ProliferationCryopreservationeducation.field_of_studyOvaryObstetrics and GynecologyFertility PreservationHodgkin DiseaseVitrificationXenograft Model Antitumor AssaysTransplantationTreatment OutcomeReproductive MedicineImmunologyCattleFemaleFertility and sterility
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Comparison of concomitant outcome achieved with fresh and cryopreserved donor oocytes vitrified by the Cryotop method

2008

Objective To evaluate the outcome of oocyte vitrification using the Cryotop method, observed in an egg donation program by simultaneously evaluating embryos derived from vitrified and fresh oocytes coming from the same stimulated cycle. Design Cohort prospective randomized study. Setting Instituto Valenciano de Infertilidad (IVI) Valencia, Spain. Patient(s) Thirty oocyte donors and 30 recipients with informed consents. Intervention(s) Vitrification by the Cryotop method. Warming 1 hour after vitrification. Microinjection of surviving MII and fresh oocytes, evaluation of fertilization, embryo development, and clinical results. Main Outcome Measure(s) Survival, fertilization, and cleavage rat…

Adultmedicine.medical_specialtyAdolescentCell Culture TechniquesFertilization in VitroBiologyCryopreservationAndrologyHuman fertilizationPregnancymedicineHumansVitrificationBlastocystCryopreservationGynecologyEstrogens Conjugated (USP)EstradiolOocyte DonationPregnancy OutcomeObstetrics and GynecologyOocyte cryopreservationEmbryo TransferOocyteCulture MediaPregnancy ratemedicine.anatomical_structureReproductive MedicineFertilizationOocytesFemaleEmbryo qualityFertility and Sterility
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Obstetric and perinatal outcome of babies born from vitrified oocytes.

2013

Objective To assess outcomes after oocyte vitrification on obstetric and perinatal outcomes compared with those achieved with fresh oocytes. Design Retrospective cohort study. Setting Private university-affiliated IVF center. Patient(s) Children born after use of vitrified oocytes (1,027 from 804 pregnancies) and fresh oocytes (1,224 from 996 pregnancies). Singleton and multiples pregnancies from own and donated ova were included. Intervention(s) Oocyte vitrification by the Cryotop method. Main Outcome Measure(s) Pregnancy, delivery, and neonatal outcomes. Result(s) Vitrification had no clinically relevant adverse effects on obstetric and perinatal outcomes after adjusting for potential con…

Adultmedicine.medical_specialtyNeonatal intensive care unitBirth weightOocyte RetrievalFertilization in VitroRisk AssessmentPregnancyRisk FactorsmedicineOdds RatioHumansAdverse effectRetrospective StudiesGynecologyCryopreservationPregnancyChi-Square Distributionbusiness.industryObstetricsPregnancy OutcomeObstetrics and GynecologyGestational ageRetrospective cohort studyOdds ratiomedicine.diseaseVitrificationConfidence intervalPregnancy ComplicationsLogistic ModelsTreatment OutcomeReproductive MedicineInfertilityMultivariate AnalysisOocytesFemalebusinessFertility and sterility
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Oocyte vitrification versus ovarian cortex transplantation in fertility preservation for adult women undergoing gonadotoxic treatments: a prospective…

2017

Objective To compare the efficacy of oocyte vitrification (OV) with that of ovarian cortex cryopreservation and transplantation (OCT) in women undergoing gonadotoxic treatments. Design Prospective observational cohort study. Setting Not applicable. Patient(s) Candidates for chemo-/radiotherapy who joined our fertility preservation (FP) program were included in this study between 2005 and 2015. One cohort included 1,024 patients undergoing OV; the other cohort included 800 patients undergoing OCT. Intervention(s) OV using the cryotop device and OCT using a slow freezing protocol. Main Outcome Measure(s) Live-birth rate (LBR) and clinical pregnancy rate (CPR). Result(s) Basal antimullerian ho…

Adultmedicine.medical_specialtyPregnancy RateAntineoplastic AgentsYoung Adult03 medical and health sciences0302 clinical medicinePregnancymedicineHumansProspective StudiesFertility preservationOvarian reserveProspective cohort studyCryopreservationGynecologyPregnancy030219 obstetrics & reproductive medicineRadiotherapybusiness.industryOvaryFertility PreservationObstetrics and Gynecologymedicine.diseaseVitrificationTransplantationFertilityTreatment OutcomeReproductive Medicine030220 oncology & carcinogenesisRelative riskCohortOocytesFemalebusinessInfertility FemaleLive BirthCohort studyFertility and Sterility
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