Search results for "TRIF"
showing 10 items of 1419 documents
Molecular Mechanism of ATP Hydrolysis in an ABC Transporter
2018
Hydrolysis of nucleoside triphosphate (NTP) plays a key role for the function of many biomolecular systems. However, the chemistry of the catalytic reaction in terms of an atomic-level understanding of the structural, dynamic, and free energy changes associated with it often remains unknown. Here, we report the molecular mechanism of adenosine triphosphate (ATP) hydrolysis in the ATP-binding cassette (ABC) transporter BtuCD-F. Free energy profiles obtained from hybrid quantum mechanical/molecular mechanical (QM/MM) molecular dynamics (MD) simulations show that the hydrolysis reaction proceeds in a stepwise manner. First, nucleophilic attack of an activated lytic water molecule at the ATP γ-…
Effect of oocyte vitrification on embryo quality: time-lapse analysis and morphokinetic evaluation.
2017
To analyze whether oocyte vitrification may affect subsequent embryo development from a morphokinetic standpoint by means of time-lapse imaging.Observational cohort study.University-affiliated private IVF center.Ovum donation cycles conducted with the use of vitrified (n = 631 cycles; n = 3,794 embryos) or fresh oocytes (n = 1,359 cycles; n = 9,935 embryos) over 2 years.None.Embryo development was analyzed in a time-lapse imaging incubator. The studied variables included time to 2 cells (t2), 3 cells (t3), 4 cells (t4), 5 cells (t5), morula (tM), and cavitated, early, and hatching blastocyst (tB, tEB, tHB) as well as 2nd cell cycle duration (cc2 = t3 - t2). All of the embryos were classifie…
Analysis of the morphological dynamics of blastocysts after vitrification/warming: defining new predictive variables of implantation
2017
Objective To describe the morphological dynamics of vitrified/warmed blastocysts and to identify quantitative morphological variables related to implantation. Subsequently, by using the most predictive parameters, to develop a hierarchical model by subdividing vitrified/warmed blastocysts into categories with different implantation potentials. Design Observational, retrospective, cohort study. Setting University-affiliated private IVF center. Patient(s) The study included 429 vitrified/warmed blastocysts with known implantation data, which were evaluated by time-lapse imaging. Blastocysts were routinely placed in EmbryoScope (Vitrolife) immediately after warming until transfer. Intervention…
EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research
2017
We argue that the field of extracellular vesicle (EV) biology needs more transparent reporting to facilitate interpretation and replication of experiments. To achieve this, we describe EV-TRACK, a crowdsourcing knowledgebase (http://evtrack.org) that centralizes EV biology and methodology with the goal of stimulating authors, reviewers, editors and funders to put experimental guidelines into practice.
A UHPLC-UV Method to Quantify Skin Deposition and Transdermal Permeation of Tizanidine Hydrochloride
2015
Tizanidine hydrochloride is an α2-adrenergic agonist used for the symptomatic relief of spasticity associated with multiple sclerosis or with spinal cord injury or disease. The objective of this study was to develop an isocratic, robust and sensitive ultra-high performance liquid chromatography method using UV detection for use in a project to develop a transdermal therapeutic system to deliver tizanidine across the skin. Isocratic separation was achieved using a C18 column and a mobile phase comprising a 80:20 mixture of 0.004% trifluoroacetic acid in water and MeCN (pH* 3.2) at a flow rate of 0.2 mL min(-1) Tizanidine eluted at 1.499 min and the total run time was 2 min. The method was sp…
The effect of peracetic acid on microbial community, water quality, nitrification and rainbow trout (Oncorhynchus mykiss) performance in recirculatin…
2020
Abstract Microbial biofilters control water quality and enable the overall function of recirculation aquaculture systems (RAS). Changes in environmental conditions can affect the abundance and interactions of the diverse microbial populations of the biofilter, affecting nitrification of harmful ammonium and thus fish health. Here, we examined the effect of different application frequencies (0, 1, 2 and 4 times per week) of a common disinfectant, peracetic acid (PAA, applied 1.1 mg l−1 twice per day), on biofilter microbial communities, focusing especially on nitrifying microbial groups and using a high throughput sequencing of 16S rRNA gene and quantitative PCR (qPCR). In addition, we measu…
CCDC 2211279: Experimental Crystal Structure Determination
2023
Related Article: Anssi Peuronen, Anni Taponen, Elina Kalenius, Ari Lehtonen, Manu Lahtinen|2023|Angew.Chem.,Int.Ed.|62|e202215689|doi:10.1002/anie.202215689
CCDC 1901898: Experimental Crystal Structure Determination
2023
Related Article: Antti J. Neuvonen, Dimitris Noutsias, Filip Topić, Kari Rissanen, Tamás Földes, Imre Pápai, Petri M. Pihko|2019|J.Org.Chem.|84|15009|doi:10.1021/acs.joc.9b01980
CCDC 1901895: Experimental Crystal Structure Determination
2023
Related Article: Antti J. Neuvonen, Dimitris Noutsias, Filip Topić, Kari Rissanen, Tamás Földes, Imre Pápai, Petri M. Pihko|2019|J.Org.Chem.|84|15009|doi:10.1021/acs.joc.9b01980
CCDC 653860: Experimental Crystal Structure Determination
2008
Related Article: A.P.Piccionello, A.Pace, S.Buscemi, N.Vivona, M.Pani|2008|Tetrahedron|64|4004|doi:10.1016/j.tet.2008.02.047