Search results for "TRM"
showing 10 items of 16 documents
Next‐Generation Sequencing‐Based RiboMethSeq Protocol for Analysis of tRNA 2′‐O‐Methylation
2017
Analysis of RNA modifications by traditional physico‐chemical approaches is labor intensive, requires substantial amounts of input material and only allows site‐by‐site measurements. The recent development of qualitative and quantitative approaches based on next‐generation sequencing (NGS) opens new perspectives for the analysis of various cellular RNA species. The Illumina sequencing‐based RiboMethSeq protocol was initially developed and successfully applied for mapping of ribosomal RNA (rRNA) 2′‐O‐methylations. This method also gives excellent results in the quantitative analysis of rRNA modifications in different species and under varying growth condi…
Mechanical Characterization of a Basalt Fabric for TRM Composites: Role of the Test Variables
2021
Textile reinforced mortar (TRM) materials are currently receiving great attention for the strengthening of reinforced concrete and masonry structural elements. Mortars with different strength classes have been coupled with different kind of synthetic fabrics, such as carbon, glass, steel, PBO. Recently natural fibres and, in particular, basalt fibres are receiving increasing interest since they proved to be a promising alternative to the most common synthetic fabrics, thanks to the reduced production costs and the consequent benefits in terms of environmental impact. This paper presents an experimental investigation comprising forty-one monotonic tensile tests on textile strips made with a …
Constitutive Models for the Tensile Behaviour of TRM Materials: Literature Review and Experimental Verification
2021
In recent years, the scientific community has focused its interest on innovative inorganic matrix composite materials, namely TRM (Textile Reinforced Mortar). This class of materials satisfies the need of retrofitting existing masonry buildings, by keeping the compatibility with the substrate. Different recent studies were addressed to improve the knowledge on their mechanical behaviour and some theoretical models were proposed for predicting the tensile response of TRM strips. However, this task is complex due to the heterogeneity of the constituent materials and the stress transfer mechanism developed between matrix and fabric through the interface in the cracked stage. This paper present…
LA SFIDA DELL'EDUCATIVO NELLA SOCIETA' LIQUIDA
2014
Le società della tarda modernità sono segnate da un deficit di educazione. L'alternativa ad una società liquida non è tanto una società solida, ma una società autentica, segnata da una ricerca del senso originario dell'educare e che assume il tempo presente, anche le sue problematicità e le incertezze, come terreno propizio per una rinnovata esistenza morale.
Lessons Learned on the Tensile and Bond Behavior of Fabric Reinforced Cementitious Matrix (FRCM) Composites
2020
Fabric Reinforced Cementitious Matrix (FRCM) composites represent an effective, compatible and cost-efficient solution for strengthening and retrofitting existing structures. A strong research effort was done to investigate the tensile and bond properties of these materials, as well as the overall behaviour of strengthened members. A Round Robin Test was organized by Rilem TC 250-CSM on 28 FRCM composites comprising basalt, carbon, glass, PBO, aramid and steel textiles, embedded in either cement, lime or geopolymer mortars, to collect an experimental dataset and define test protocols. This paper collects the outcomes of this study to highlight fundamental properties of FRCM and to investiga…
Effects of Different Test Setups on the Experimental Tensile Behaviour of Basalt Fibre Bidirectional Grids for FRCM Composites
2020
Fibre-reinforced cementitious matrix (FRCM) composites have been effectively used during the last ten years for the strengthening of existing concrete and masonry structures. These composite materials are made of medium- and high-strength fibre meshes embedded in inorganic matrices. Synthetic fibres are the ones that are currently the most used
Effect of corner over-reinforcing strips on the compressive behaviour of TRM confined masonry columns
2020
Several recent works studied the efficiency of inorganic matrix composites, namely Textile Reinforced Mortar (TRM) or Fabric Reinforced Cementitious Matrix, for enhancing the strength and shortening capacity of masonry columns subjected to axial load. Literature studies were addressed to study the great number of variables involved in the problem, such as the nature and the grade of mortar, the strength of the fabric, the number of reinforcing layers, the type, the arrangement and the strength of the masonry, and helped to draw the first technical guidelines for practitioners and designers. All the experimental works highlighted that the actual performance of TRM confinement in masonry memb…
Structural insights into the GTPase domain of Escherichia coli MnmE protein
2007
The Escherichia coli MnmE protein is a 50-kDa multidomain GTPase involved in tRNA modification. Its homologues in eukaryotes are crucial for mitochondrial respiration and, thus, it is thought that the human protein might be involved in mitochondrial diseases. Unlike Ras, MnmE shows a high intrinsic GTPase activity and requires effective GTP hydrolysis, and not simply GTP binding, to be functionally active. The isolated MnmE G-domain (165 residues) conserves the GTPase activity of the entire protein, suggesting that it contains the catalytic residues for GTP hydrolysis. To explore the GTP hydrolysis mechanism of MnmE, we analyzed the effect of low pH on binding and hydrolysis of GTP, as well…
A new method for fluoride determination by using fluorophores and dyes anchored onto MCM-41
2002
A new colourimetric and fluorimetric method for fluoride determination in aqueous samples based on the specific reaction between fluoride and silica has been developed and applied on real samples. Descalzo Lopez, Ana Belen, adeslo@alumni.uv.es ; El Haskouri, Jamal, Jamal.Haskouri@uv.es ; Beltran Porter, Daniel, Daniel.Beltran@uv.es ; Amoros del Toro, Pedro Jose, Pedro.Amoros@uv.es
Next-Generation Sequencing-Based RiboMethSeq Protocol for Analysis of tRNA 2'-O-Methylation.
2016
Analysis of RNA modifications by traditional physico-chemical approaches is labor intensive, requires substantial amounts of input material and only allows site-by-site measurements. The recent development of qualitative and quantitative approaches based on next-generation sequencing (NGS) opens new perspectives for the analysis of various cellular RNA species. The Illumina sequencing-based RiboMethSeq protocol was initially developed and successfully applied for mapping of ribosomal RNA (rRNA) 2'-O-methylations. This method also gives excellent results in the quantitative analysis of rRNA modifications in different species and under varying growth condi…