Search results for "Technical Note"
showing 10 items of 31 documents
Nuclear and Cytoplasmic Soluble Proteins Extraction from a Small Quantity of Drosophila’s Whole Larvae and Tissues
2015
The identification and study of protein’s function in several model organisms is carried out using both nuclear and cytoplasmic extracts. For a long time, Drosophila’s embryos have represented the main source for protein extractions, although in the last year, the importance of collecting proteins extracts also from larval tissues has also been understood. Here we report a very simple protocol, improved by a previously developed method, to produce in a single extraction both highly stable nuclear and cytoplasmic protein extracts from a small quantity of whole Drosophila’s larvae or tissues, suitable for biochemical analyses like co-immunoprecipitation.
Autostereoscopic Three-Dimensional Neuronavigation to the Sella: Technical Note.
2017
Background A drawback of conventional neuronavigation is the necessity of focusing on two-dimensional images in 3 planes at the same time to determine one's position in the operating field. A solution would be to merge the images into a single three-dimensional (3D) image that mirrors the actual anatomy. The introduction of holographic glassless 3D monitors paved the way to 3D navigation. We present our experience with 3D neuronavigation as exemplified by navigation to and within the sella. Methods Operative planning was conducted with a navigation system using cranial computed tomography and magnetic resonance imaging. The image data sets were processed by the prototype Clariti 3D system t…
Technical note: a comparison of central and peripheral intraocular pressure using rebound tonometry
2007
Purpose: To compare central and peripheral intraocular pressure (IOP) readings obtained with rebound tonometry.Methods: Intraocular pressure was measured on the right eye of 153 patients (65 males, 88 females), aged from 21 to 85 years (mean +/- S.D., 55.5 +/- 15.2 years) with the ICare rebound tonometer at centre, and 2 mm from the limbus (in the nasal and temporal regions along the 0-180O corneal meridian).Results: Intraocular pressure values obtained with the ICare were 14.9 +/- 2.8; 14.1 +/- 2.5 and 14.5 +/- 2.7 mmHg at centre, nasal and temporal corneal locations, respectively. On average, nasal and temporal IOP readings were 0.75 and 0.37 mmHg lower than the central reading (p 0.05, r…
Technical Note: Comparison of Techniques for Evaluating the Relative Preference by Sheep Among Saltbush Clones
2004
This research compared 4 field methods of evaluating the relative preference by sheep of 28 clones of saltbush (Atriplex halimus L.). The methods were as follows. 1) Leaf dots (LD): 8 leaves per shrub were marked on the lower surface with a small dot using a water-resistant, nontoxic ink. 2) Twig marks (TM): 2 current-year twigs per shrub were marked with 3 lines using the same ink approximately in the middle of the basal, median, and apical thirds. 3) Branch length (BL): 2 branches per shrub were marked with ink at the base of the current year's growth. The twigs were measured from the marked point to the top, before and after sheep browsing. 4) Ocular estimation (OE): the percentage of th…
Technical Note: Minimal Access Surgery for Cochlear Implantation with MED-EL Devices
2005
<i>Background:</i> Minimally invasive techniques have been described for cochlear implant surgery, but so far this had not been the case for Med-EL devices. <i>Objective:</i> To describe a newly developed minimal access approach for the implantation of Med-EL devices and report our results after up to 1 year of follow-up in 52 patients. <i>Discussion:</i> The use of a minimally invasive approach without raising a flap or extensive drilling of a bony well was feasible in all 52 patients of this series. It shortened the surgical time to an average of 45 min and there were no specific postoperative complications. The average follow-up of 8.4 months was uneve…
Use of a new aneurysm clip with an inverted-spring mechanism to facilitate visual control during clip application. Technical note.
1995
✓ When operating on deep-seated cerebral aneurysms, the surgeon's visual control of clip application may be impaired by the clip holder unless adjacent structures are retracted. To improve visual control and reduce the necessity for retraction, the senior author (A.P.) developed a new concept: an aneurysm clip with an inverted-spring mechanism. The clip has two jaws that point away from the clip blades. The jaws of the clip holder articulate with the inner side of the clip jaws. By distending the jaws of the clip holder the blades of the clip are opened and vice versa. Thus the visual field increases while the clip application is proceeding. This instrumentation is useful, especially in cas…
mBISON: Finding miRNA target over-representation in gene lists from ChIP-sequencing data
2015
Background Over-representation of predicted miRNA targets in sets of genes regulated by a given transcription factor (e.g. as defined by ChIP-sequencing experiments) helps to identify biologically relevant miRNA targets and is useful to get insight into post-transcriptional regulation. Findings To facilitate the application of this approach we have created the mBISON web-application. mBISON calculates the significance of over-representation of miRNA targets in a given non-ranked gene set. The gene set can be specified either by a list of genes or by one or more ChIP-seq datasets followed by a user-defined peak-gene association procedure. mBISON is based on predictions from TargetScan and us…
Technical Note:Study of the Cl−-Induced Breakdown of the Passive Layer on Steel
1991
Abstract The Cl−-induced breakdown of the passive layer on a standard steel has an induction time that increases in a nearly linear fashion with the previous holding time (at the passivation potent...
Short-duration rainfalls in Sicily
1990
The Italian Hydrographic Service publishes yearly maximum rainfall depths with 1-, 3-, 6-, 12-, and 24-hr duration, recorded by recording rain gauges. It also publishes selected short-duration (t<1 hr) rainfall amounts. It is impossible to carry out a statistical analysis of the short-duration rainfalls for each recording rain gauge because the published data correspond to different record durations and to different recording rain gauges from year to year. Therefore, there is a practical need to derive empirical relationships between the 1-, 3-, 6-, 12-, and 24-hr duration amounts and shorter duration (t<1 hr) rainfall amounts. Such a relationship has been proposed by Bell and has been veri…
Grapevine and wine metabolomics-based guidelines for fair data and metadata management
2021
In the era of big and omics data, good organization, management, and description of experimental data are crucial for achieving high-quality datasets. This, in turn, is essential for the export of robust results, to publish reliable papers, make data more easily available, and unlock the huge potential of data reuse. Lately, more and more journals now require authors to share data and metadata according to the FAIR (Findable, Accessible, Interoperable, Reusable) principles. This work aims to provide a step-by-step guideline for the FAIR data and metadata management specific to grapevine and wine science. In detail, the guidelines include recommendations for the organization of data and meta…