Search results for "Teratozoospermia"
showing 4 items of 4 documents
Diagnostic genetic screening for assisted reproductive technologies patients with macrozoospermia
2017
International audience; Macrozoospermia is characterized by a high proportion of abnormal spermatozoa with enlarged heads. So far, it has been associated with mutations only in the Aurora Kinase C gene (AURKC) in some cases. Although many publications have reported failure to conceive in couples with macrozoospermia, a few others have described successful pregnancies, thus raising questions as to whether ICSI and AURKC genetic screening should be recommended in all patients with macrozoospermia. First, we report on two monozygotic twins presenting macrozoospermia for whom the genetic status was explored (Aurora Kinase C sequencing) and whole semen and gradient-selected spermatozoa were anal…
Sperm Telomere Length in Men with Normal and Abnormal semen Parameters. A Pilot Study
2019
Objective: Male infertility is a growing social problem. The measurement of telomere length may be a new marker that can help to assess reproductive age of males and assess the chances of in vitro fertilization. Aim: The aim of our study was to investigate whether there are any differences between telomere length in men with normal and abnormal semen parameters. Materials and Methods: Telomere length was analyzed in 17 patients with normal and 28 patients with abnormal semen parameters using the telomeric repeat amplification protocol and Quantitative Real-Time Polymerase Chain Reaction (qPCR). Results: Mean sperm telomere length in all donors was 49±24kbp per haploid genome. However, patie…
A new technique for analysis of human sperm morphology in unstained cells from raw semen
2014
Sperm morphology analysis is a fundamental component of semen analysis, but its real significance has been clouded by the plethora of techniques used for its evaluation. Most involve different fixation and staining procedures that induce artefacts. Herein we describe Trumorph (Proiser R+D, Paterna, Spain), a new method for sperm morphology analysis based on examination of wet preparations of spermatozoa immobilised, after a short 60°C shock, in narrow chambers and examined by negative phase contrast microscopy. A range of morphological forms was observed, similar to those found using conventional fixed and stained preparations, but other forms were also found, distinguishable only by the o…
Computer assisted morphometric analysis of ram sperm heads: Evaluation of different fixative techniques
1998
The recent development of automated systems for morphometric sperm head analysis has provided a series of objective parameters which have facilitated the standardization of morphological semen evaluation. This current work attempts to establish the optimum fixing conditions for the morphometric characterization of ram spermatozoa. Ejaculates were obtained from 5 Merino rams used for periodic collection of semen and were diluted at 1:50 with TEST medium. Air-dried smears were fixed either in ethanol-ether (1:1), 50% methanol, 2% glutaraldehyde or SUZA fixative, in which case the smear was pretreated with chloramine. The samples were then stained with commercial kit Hemacolor. Once the prepar…