Search results for "Terminal"
showing 10 items of 364 documents
Pre- and postsynaptic type-1 cannabinoid receptors control the alterations of glutamate transmission in experimental autoimmune encephalomyelitis
2013
Type-1 cannabinoid receptors (CB1R) are important regulators of the neurodegenerative damage in multiple sclerosis (MS) and in experimental autoimmune encephalomyelitis (EAE). In GABAergic striatal neurons, CB1R stimulation exerts protective effects by limiting inflammation-induced potentiation of glutamate-mediated spontaneous excitatory postsynaptic currents (sEPSCs). Here we show that CB1R located on GABAergic or on glutamatergic neurons are differentially involved in the pre- and postsynaptic alterations of sEPSCs caused by EAE in the striatum. After induction of EAE, mice selectively lacking CB1R on GABAergic neurons (GABA-CB1R-KO) showed exacerbated alterations of sEPSC duration in GA…
Recycled Tyre Rubber Modified Bitumens for road asphalt mixtures: A literature review
2013
AbstractNowadays, only a small percentage of waste tyres are being land-filled. The Recycled Tyre Rubber is being used in new tyres, in tyre-derived fuel, in civil engineering applications and products, in moulded rubber products, in agricultural uses, recreational and sports applications and in rubber modified asphalt applications. The benefits of using rubber modified asphalts are being more widely experienced and recognized, and the incorporation of tyres into asphalt is likely to increase. The technology with much different evidence of success demonstrated by roads built in the last 40years is the rubberised asphalt mixture obtained through the so-called “wet process” which involves the…
Oligomerization and hemolytic properties of the C-terminal domain of pyolysin, a cholesterol-dependent cytolysin
2013
Pyolysin (PLO) belongs to the homologous family of the cholesterol- dependent cytolysins (CDCs), which bind to cell membranes containing cholesterol to form oligomeric pores of large size. The CDC monomer structure consists of 4 domains. Among these, the C-terminal domain 4 has been implicated in membrane binding of the monomer, while the subsequent processes of oligomerization and membrane insertion have primarily been assigned to other domains of the molecule. Recombinantly expressed or proteolytic fragments that span domain 4 of the CDCs streptolysin O and perfringolysin O bind to membranes but fail to oligomerize, and they inhibit the activity of the respective wild-type toxins. We repo…
Ethical User stories : Industrial study
2022
Publisher Copyright: © 2022 Copyright for this paper by its authors In Port terminals a progressive change is underway in digitalizing traditional systems to SMART systems with the aid of AI. This study follows one of such progressions, the SMARTER project. SMARTER is a sub research and development project of the Sea for Value program of DIMECC company, Finland to create replicable models for digitalization for future terminals which involves the use of AI enabled tools. AI and Autonomous Systems (AS) are the direction that software systems are taking today. But due to ethical challenges involved in the use of AI systems and increased emphasis on ethical practices in the use and design of A…
A Stackelberg-game approach to support the design of logistic terminals
2014
This paper deals with the design of logistic terminals taking Sicily, in the South of Italy, as a case study. It focuses on consolidation terminals for truckers and addresses the problem of optimising location pattern and public share in investments. This problem is solved through a Stackelberg game between the designer and the collective of road carriers. So a bilevel approach combines a system-optimum problem, at the upper level, with the carrier equilibrium problem, at the lower level. The choice behaviour of the lower-level player is simulated by a random utility model. The output of the game suggests that private companies and society should share the investments and the public contrib…
Protection of HeLa‐T4 + cells against human immunodeficiency virus (HIV) infection after stable transfection with HIV LTR‐2‘,5‘‐oligoadenylate synthe…
1990
An expression vector (pU3R-III/2-5AS) of human 2',5'-oligoadenylate (2-5A) synthetase was constructed in which a cDNA encoding an active form of the enzyme was located 3' to a 3'-long terminal repeat (LTR) of human immunodeficiency virus type 1 (HIV-1). The LTR-directed expression of this hybrid DNA could be activated in trans by the HIV tat gene product. This vector was used for transfection of HeLa-T4+ cells, which are permissive to HIV infection, as well as of normal HeLa cells. HIV replication after infection of the CD4-receptor-bearing HeLa-T4+ cells with HIV-1 was found to be strongly reduced when drug-selected cells cotransfected with pU3R-III/2-5AS and a hygromycin B resistance gene…
Network dynamics of eukaryotic LTR retroelements beyond phylogenetic trees
2009
Abstract Background Sequencing projects have allowed diverse retroviruses and LTR retrotransposons from different eukaryotic organisms to be characterized. It is known that retroviruses and other retro-transcribing viruses evolve from LTR retrotransposons and that this whole system clusters into five families: Ty3/Gypsy, Retroviridae, Ty1/Copia, Bel/Pao and Caulimoviridae. Phylogenetic analyses usually show that these split into multiple distinct lineages but what is yet to be understood is how deep evolution occurred in this system. Results We combined phylogenetic and graph analyses to investigate the history of LTR retroelements both as a tree and as a network. We used 268 non-redundant …
Mutational analysis of eggplant latent viroid RNA processing in Chlamydomonas reinhardtii chloroplast.
2009
Viroids of the family Avsunviroidae, such as eggplant latent viroid (ELVd), contain hammerhead ribozymes and replicate in the chloroplasts of the host plant through an RNA-based symmetrical rolling-circle mechanism in which oligomeric RNAs of both polarity are processed to monomeric linear RNAs (by cleavage) and to monomeric circular RNAs (by ligation). Using an experimental system consisting of transplastomic lines of the alga Chlamydomonas reinhardtii, a mutational analysis of sequence and structural elements in the ELVd molecule that are involved in transcript processing in vivo in a chloroplastic context was carried out. A collection of six insertion and three deletion ELVd mutants was …
A mammalian gene evolved from the integrase domain of an LTR retrotransposon.
2001
FIG. 1.—Summary of the structure and coding sequence of the human Gin-1 gene. Sequences of human cDNAs with accession numbers XMp003947.2 (a putative full-length cDNA), BE502574, AW173201.1, AW950418.1, AI631948.1, and AA766836.1 were used to deduce and confirm these data. The full-length protein is 522 amino acids long. The Gin-1 coding region spans nucleotides 36153–15345 in the genomic clone NTp002663.4. Arrowheads and the numbers above them, respectively, indicate the positions and lengths of introns. Several Alu repeats were detected within the two largest introns. Bold letters indicate the region homologous to the most conserved part of the IN domain, detailed in figure 2 and used to …
GIS-data related route optimization, hierarchical clustering, location optimization, and kernel density methods are useful for promoting distributed …
2019
Currently, geographic information system (GIS) models are popular for studying location-allocation-related questions concerning bioenergy plants. The aim of this study was to develop a model to investigate optimal locations for two different types of bioenergy plants, for farm and centralized biogas plants, and for wood terminals in rural areas based on minimizing transportation distances. The optimal locations of biogas plants were determined using location optimization tools in R software, and the optimal locations of wood terminals were determined using kernel density tools in ArcGIS. The present case study showed that the utilized GIS tools are useful for bioenergy-related decision-maki…