Search results for "Testis"

2002

Analysis of gonad organization and development revealed that Serranus atricauda is a functional simultaneous hermaphrodite. The ovary is classified as asynchronous. Oocyte growth is divided into five stages: stage I (primary growth stage), stage II (yolk vesicle formation), stage III (vitellogenesis), stage IV (oocyte maturation) and stage V (mature egg). The testis is of the unrestricted or lobular spermatogonial type. Spermatogenic cells include spermatogonia, primary and secondary spermatocytes, spermatids (which form cysts in the seminiferous tubules) and spermatozoa (free in the lumen). The histological structure of the gonad and sperm morphology would seem to indicate that this specie…

Absence of mutations in the WT1 gene in patients with XY gonadal dysgenesis

1995

The WT1 gene is normally expressed during gonadal development and specific mutations in heterozygous form cause Drash syndrome, characterized by male pseudohermaphroditism and gonadal dysgenesis, renal failure and a predisposition for Wilms' tumour. These observations prompted us to test whether WT1 mutations are involved in isolated gonadal dysgenesis, being the most severe form of disturbance in gonadal differentiation. We studied 27 cases of 46,XY females with gonadal dysgenesis who had previously been screened for and found not to carry SRY gene mutations. We performed mutational screening of the WT1 gene with denaturing gradient gel electrophoresis. In one of these patients, a heterozy…

Lasten tasapainon laadullisten piirteiden havainnoinnissa apuna käytettävän arviointilomakkeen kehittäminen ja testaus

2001

4-vuotiaiden lasten motorisissa perustaidoissa tapahtuneet muutokset 20 vuoden aikana

2013

Protandric hermaphrodite peculiarities in Amphiprion frenatus Brevoort (Teleostei, Pomacentridae)

1990

Electron microscopy of the male phase of the ovotestis of Amphiprion frenatus, a protandric hermaphrodite, showed no connective tissue between male and female areas and, as the basal lamina was lacking both along the seminiferous tubules and round the previtellogenic oocytes, the male and female germ cells were only separated by their respective surrounding somatic cells (Sertoli and follicle cells). Besides previtellogenic oocytes, oocytes in meiotic prophase and very small (young) previtellogenic oocytes, were detected in the ovarian part, as spermatogenesis proceeded, revealing oogenetic activity. Degeneration of some previtellogenic oocytes and their follicle cells was discernible.

Identification of a new nonsense mutation (Tyr129Stop) of the SRY gene in a newborn infant with XY sex-reversal.

2004

Point mutations and deletions of SRY gene have been described in several cases of XY gonadal dysgenesis. To date, most of these mutations affect the HMG domain of SRY which plays a central role in DNA binding activity of SRY. We report on a non-mosaic XY sex-reversed newborn girl (completely female external genitalia). The direct sequencing of SRY showed a new nonsense mutation in a codon of SRY gene flanking the 3' end of the HMG domain: a thymine is replaced by a guanine at position +387 in codon 129, resulting in the replacement of the amino acid tyrosine (TAT) by a stop codon (TAG). The new mutation of this patient provides further evidence to support the functional importance of the pu…

Analysis of Exosomal Cargo Provides Accurate Clinical, Histologic and Mutational Information in Non-Small Cell Lung Cancer

2022

Simple Summary Non-small cell lung cancer (NSCLC) is the second most commonly diagnosed cancer and the leading cause of cancer-related death worldwide. Clinical decision-making depends on the histological classification; however, tissue biopsy is frequently not technically feasible due to tumor location or limited tissue samples. Therefore, we propose to find clinical, molecular and histological biomarkers using a minimally invasive approach based on the analysis of the cargo of the blood extracellular vesicles. Exosomes are membranous vesicles present in several biological fluids, which carry biological information to distant tissues, regulating several tumor processes. This study aims to …