Search results for "Throughput"

Human Leukocyte Antigen Typing Using High-Throughput DNA and RNA Sequencing and Application for Cell Line Identification

2019

Current development of CFTR potentiators in the last decade

2020

Cystic fibrosis (CF) is a genetic disorder produced by the loss of function of CFTR, a main chloride channel involved in transepithelial salt and water transport. CFTR function can be rescued by small molecules called "potentiators" which increase gating activity of CFTR on epithelial surfaces. High throughput screening (HTS) assays allowed the identification of new chemical entities endowed with potentiator properties, further improved through medicinal chemistry optimization. In this review, the most relevant classes of CFTR potentiators developed in the last decade were explored, focusing on structure-activity relationships (SAR) of the different chemical entities, as a useful tool for t…

ANKRD26-RET - A novel gene fusion involving RET in papillary thyroid carcinoma

2018

Abstract Background Rearrangements of RET are drivers of oncogenesis, traceable in different cancer types as papillary thyroid carcinoma (PTC), non-small cell lung cancer, colorectal or breast cancer. Anchored multiplex PCR based next-generation sequencing (NGS) can detect RET rearrangements involving previously unknown partner genes. Methods A sample of PTC underwent NGS, following detection of RET rearrangement by fluorescence in situ hybridization. Expression analysis of ANKRD26 and RET was performed for the tumor harboring ANKRD26-RET, for corresponding normal thyroid tissue and PTC tumors with representative genetic alterations (BRAFV600E, CCDC6-RET), complemented by a comparative sear…

Consistency of Targeted Metatranscriptomics and Morphological Characterization of Phytoplankton Communities

2020

The composition of phytoplankton community is the basis for environmental monitoring and assessment of the ecological status of aquatic ecosystems. Community composition studies of phytoplankton have been based on time-consuming and expertise-demanding light microscopy analyses. Molecular methods have the potential to replace microscopy, but the high copy number variation of ribosomal genes and the lack of universal primers for simultaneous amplification of prokaryotic and eukaryotic genes complicate data interpretation. In this study, we used our previously developed directional primer-independent high-throughput sequencing (HTS) approach to analyze 16S and 18S rRNA community structures. C…

Transmission Optimization and Resource Allocation for Wireless Powered Dense Vehicle Area Network With Energy Recycling

2022

The wireless-powered communication paradigm brings self-sustainability to the on-vehicle sensors by harvesting the energy from radiated radio frequency (RF) signals. This paper proposes a novel transmission and resource allocation strategy for the scenario where multiple wireless powered vehicle area networks (VAN) co-existed with high density. The considered multi-VAN system consists of a remote master access point (MAP), multiple on-vehicle hybrid access points (HAPs) and sensors. Unlike previous works, we consider that the sensors can recycle the radiated radio frequency energy from all the HAPs when HAPs communicate with MAP, so the dedicated signals for energy harvesting (EH) are unnec…

A game theoretic approach to MAC design for infrastructure networks

2010

Wireless network operation intrinsically assumes different forms of cooperation among the network nodes, such as sharing a common wireless medium without interfering, relaying frames belonging to other nodes, controlling the transmission power for optimizing spectrum reuse, coding cooperatively multiple frames for improving information redundancy, and so on. For this reason, Game Theory has been extensively employed to model wireless networks. In particular, we propose a game-theoretic approach for defining a generalized medium access protocol for slotted contention-based channels. Contention-based channels are largely adopted in data networks, e.g. in WiFi and WiMax networks and in some em…

Short-term impact of biogas digestates on soils microbial communities

2022

International audience; Anaerobic digestion of organic waste is considered a key process to produce renewable energy to meet the growing sustainable energy demand. Digestates can be used in agriculture as soil amendments and improve crop yields. However, their use at large scale in agricultural fields still requires to prove their innocuity on soil biota, especially on microorganisms that play important roles in soil ecosystem. Here, we designed a microcosm experiment to compare the short-term (42-days) effects of four different digestates (derived from cattle manure, energy crop, food residues or slurry with bio-waste) on the soil microbial communities. Each digestate was applied on three …

'Good to Repeat': Making Random Access Near-Optimal with Repeated Contentions

2020

Recent advances on WLAN technology have been focused mostly on boosting network capacity by means of a more efficient and flexible physical layer. A new concept is required at MAC level to exploit fully the new capabilities of the PHY layer. In this article, we propose a contention mechanism based on Repeated Contentions (ReCo) in frequency domain. It provides a simple-to-configure, robust and short-term fair algorithm for the random contention component of the MAC protocol. The throughput efficiency of ReCo is not sensitive to the number of contending stations, so that ReCo does not require adaptive tuning of the access parameters for performance optimization. Efficiency and robustness is …

Control of Murine Cytomegalovirus Infection by γδ T Cells

2015

Infections with cytomegalovirus (CMV) can cause severe disease in immunosuppressed patients and infected newborns. Innate as well as cellular and humoral adaptive immune effector functions contribute to the control of CMV in immunocompetent individuals. None of the innate or adaptive immune functions are essential for virus control, however. Expansion of γδ T cells has been observed during human CMV (HCMV) infection in the fetus and in transplant patients with HCMV reactivation but the protective function of γδ T cells under these conditions remains unclear. Here we show for murine CMV (MCMV) infections that mice that lack CD8 and CD4 αβ-T cells as well as B lymphocytes can control a MCMV i…

Pole-to-Pole Connections: Similarities between Arctic and Antarctic Microbiomes and Their Vulnerability to Environmental Change

2017

The global biogeography of microorganisms remains poorly resolved, which limits the current understanding of microbial resilience toward environmental changes. Using high-throughput 16S rRNA gene amplicon sequencing, we characterized the microbial diversity of terrestrial and lacustrine biofilms from the Arctic, Antarctic and temperate regions. Our analyses suggest that bacterial community compositions at the poles are more similar to each other than they are to geographically closer temperate habitats, with 32% of all operational taxonomic units (OTUs) co-occurring in both polar regions. While specific microbial taxa were confined to distinct regions, representing potentially endemic popul…