Search results for "Thy-1"

showing 5 items of 15 documents

Langerin+ DCs regulate innate IL-17 production in the oral mucosa during Candida albicans-mediated infection

2018

The opportunistic fungal pathogen Candida albicans frequently causes diseases such as oropharyngeal candidiasis (OPC) in immunocompromised individuals. Although it is well appreciated that the cytokine IL-17 is crucial for protective immunity against OPC, the cellular source and the regulation of this cytokine during infection are still a matter of debate. Here, we directly visualized IL-17 production in the tongue of experimentally infected mice, thereby demonstrating that this key cytokine is expressed by three complementary subsets of CD90+ leukocytes: RAG-dependent αβ and γδ T cells, as well as RAG-independent ILCs. To determine the regulation of IL-17 production at the onset of OPC, we…

Malemedicine.medical_treatment2405 ParasitologyPathology and Laboratory Medicine10263 Institute of Experimental ImmunologyMonocytesMice0302 clinical medicineAnimal CellsCandida albicansBiology (General)Candida albicansMononuclear Phagocyte SystemFungal PathogensInnate Immune Systemeducation.field_of_studyEukaryotaMononuclear phagocyte systemFlow CytometryCorpus albicans3. Good healthSpectrophotometryMedical MicrobiologyCytokinesCytophotometryCellular Types10244 Institute of VirologyQH301-705.5Immune CellsImmunologyMicrobiology03 medical and health sciences1311 GeneticsGenetics1312 Molecular BiologyeducationMicrobial PathogensMolecular BiologyMouth2403 ImmunologyBlood CellsOrganismsBiology and Life SciencesDendritic CellsMolecular DevelopmentYeastMice Inbred C57BLMannose-Binding Lectins030104 developmental biologyImmunologyThy-1 Antigens570 Life sciences; biologyParasitologyImmunologic diseases. AllergyDigestive SystemDevelopmental Biology0301 basic medicineNeutrophilsPhysiologyInterleukin-1betaYeast and Fungal ModelsInterleukin-23White Blood CellsSpectrum Analysis TechniquesCandidiasis OralImmune PhysiologyLeukocytesMedicine and Health SciencesCandidaStainingbiologyInterleukin-172404 MicrobiologyCell StainingSpecific Pathogen-Free OrganismsInfectious DiseasesCytokineExperimental Organism SystemsAntigens SurfaceFemaleAnatomyPathogensResearch ArticleLangerinPopulationMycologyOpportunistic InfectionsResearch and Analysis MethodsTongueImmunityVirologymedicineAnimalsLectins C-TypeInterleukin 6Interleukin-6Mouth MucosaFungiCell BiologyRC581-607biology.organism_classificationSpecimen Preparation and TreatmentImmune Systembiology.protein2406 VirologySpleen030215 immunology
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Wharton's Jelly Stem Cells: A Novel Cell Source for Oral Mucosa and Skin Epithelia Regeneration

2013

Abstract Perinatal stem cells such as human umbilical cord Wharton's jelly stem cells (HWJSCs) are excellent candidates for tissue engineering because of their proliferation and differentiation capabilities. However, their differentiation potential into epithelial cells at in vitro and in vivo levels has not yet been reported. In this work we have studied the capability of HWJSCs to differentiate in vitro and in vivo to oral mucosa and skin epithelial cells using a bioactive three-dimensional model that mimics the native epithelial-mesenchymal interaction. To achieve this, primary cell cultures of HWJSCs, oral mucosa, and skin fibroblasts were obtained in order to generate a three-dimension…

Pathologymedicine.medical_specialtyFluorescent Antibody TechniqueMice NudeFilaggrin ProteinsBiologyModels BiologicalEpitheliumMiceIntermediate Filament ProteinsTissue engineeringTissue Engineering and Regenerative MedicineWharton's jellymedicineAnimalsHumansRegenerationWharton JellyProtein PrecursorsOral mucosaInvolucrinSkinRegeneration (biology)Mouth MucosaCell DifferentiationEpithelial CellsMesenchymal Stem CellsCell BiologyGeneral MedicineFlow CytometryCell biologymedicine.anatomical_structureCell cultureKeratinsLeukocyte Common AntigensThy-1 Antigensgamma CateninStem cellDevelopmental BiologyAdult stem cellStem Cells Translational Medicine
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Enrichment of Retinal Ganglion Cells in Rat Retinal Lysate by Excimer Laser Ablation of the Outer Retina

2013

PURPOSE. Retinal ganglion cells (RGC) are a relatively small cell population in the retina. This leads to an unfavorable signal-tonoise ratio when analyzing RGC proteins in whole retina lysate. We present a novel technique to obtain RGC-enriched rat retinal lysate by removing the outer retinal layers with an excimer laser before lysation. METHODS. Outer retinal layers were ablated with an excimer laser on flat mounted retinas from adult albino rats. 4 0 6Diamidino-2-phenylindole dihydrochloride hydrate (DAPI) nuclear staining was used to assess the ablation efficacy (n ! 6). Western blot for layer specific markers (rhodopsin, parvalbumin, b-III-tubulin) was performed to quantify changes in …

Retinal Ganglion CellsRhodopsingenetic structuresBlotting WesternPopulationRetinal ganglionRetina03 medical and health scienceschemistry.chemical_compound0302 clinical medicineTubulinmedicineAnimalseducationGanglion cell layerRetinaeducation.field_of_studyLaser ablationbiologyRetinalAnatomyCREB-Binding ProteinMolecular biologyeye diseasesRatsmedicine.anatomical_structurechemistryRhodopsin030221 ophthalmology & optometrybiology.proteinOptic nerveThy-1 AntigensLaser Therapysense organs030217 neurology & neurosurgeryInvestigative Opthalmology & Visual Science
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CCDC 1509299: Experimental Crystal Structure Determination

2016

Related Article: M.A. Harvey, S. Suarez, R. Baggio|2016|Acta Crystallogr.,Sect.E:Cryst.Commun.|72|1581|doi:10.1107/S2056989016016029

Space GroupCrystallographyCrystal Systembis(3478-tetramethy-110-phenanthroline)-(peroxodisulfato)-cadmium(ii)Crystal StructureCell ParametersExperimental 3D Coordinates
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CCDC 1509298: Experimental Crystal Structure Determination

2016

Related Article: M.A. Harvey, S. Suarez, R. Baggio|2016|Acta Crystallogr.,Sect.E:Cryst.Commun.|72|1581|doi:10.1107/S2056989016016029

Space GroupCrystallographyCrystal Systembis(3478-tetramethy-110-phenanthroline)-(peroxodisulfato)-zinc(ii)Crystal StructureCell ParametersExperimental 3D Coordinates
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