Search results for "Time-resolved"

showing 10 items of 70 documents

The Monod-Wyman-Changeux allosteric model accounts for the quaternary transition dynamics in wild type and a recombinant mutant human hemoglobin

2012

International audience; The acknowledged success of the Monod-Wyman-Changeux (MWC) allosteric model stems from its efficacy in accounting for the functional behavior of many complex proteins starting with hemoglobin (the paradigmatic case) and extending to channels and receptors. The kinetic aspects of the allosteric model, however, have been often neglected, with the exception of hemoglobin and a few other proteins where conformational relaxations can be triggered by a short and intense laser pulse, and monitored by time-resolved optical spectroscopy. Only recently the application of time-resolved wide-angle X-ray scattering (TR-WAXS), a direct structurally sensitive technique, unveiled th…

Models MolecularProtein ConformationcooperativityMESH: Catalytic DomainCooperativity01 natural sciencesMESH: Recombinant ProteinsHemoglobinsProtein structureMESH: Protein ConformationCatalytic Domainprotein structural dynamicsMESH: Allosteric Site0303 health sciencesMultidisciplinaryallosterybiologyMESH: KineticsChemistryBiological SciencesRecombinant Proteins[SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry Molecular Biology/BiophysicsMESH: HemoglobinsAllosteric SiteMESH: Models MolecularAdultMESH: MutationStereochemistryKineticsAllosteric regulation010402 general chemistry03 medical and health sciencesprotein conformational changesflash photolysisallostery; cooperativity; flash photolysis; hemoglobin; protein conformational changes; protein structural dynamics; time-resolved wide angle x ray scattering; time-resolved x-ray scatteringHumans030304 developmental biologytime-resolved X-ray scattering; protein conformational changes; cooperativity; flash photolysisMESH: Humanstime-resolved X-ray scatteringWild typeActive sitetime-resolved wide angle x ray scatteringMESH: AdulthemoglobinSettore FIS/07 - Fisica Applicata(Beni Culturali Ambientali Biol.e Medicin)0104 chemical sciencesprotein conformational changeKineticsAllosteric enzymeMutationbiology.proteinHemoglobin
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Mass measurement of cooled neutron-deficient bismuth projectile fragments with time-resolved Schottky mass spectrometry at the FRS-ESR facility

2005

Masses of 582 neutron-deficient nuclides ($30\leq{Z}\leq{85}$) were measured with time-resolved Schottky mass spectrometry at the FRS-ESR facility at GSI, 117 were used for calibration. The masses of 71 nuclides were obtained for the first time. A typical mass accuracy of 30 $\mu$u was achieved. These data have entered the latest atomic mass evaluation. The mass determination of about 140 additional nuclides was possible via known energies ($Q$-values) of $\alpha-$, $\beta-$, or proton decays. The obtained results are compared with the results of other measurements.

Nuclear and High Energy PhysicsA=79–207ProtonAtomic massesNuclear Theorychemistry.chemical_element[PHYS.NEXP]Physics [physics]/Nuclear Experiment [nucl-ex]Mass spectrometry01 natural sciences7. Clean energyBismuthmassesNuclear physicsZ=30–850103 physical sciencesNeutronNuclide010306 general physicsNuclear ExperimentPhysics010308 nuclear & particles physicsTime-resolved Schottky mass spectrometrySchottky diodeAtomic massMasschemistrymeasured
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From radop to laser spectroscopy and back

1985

The paper reviews some techniques in optical spectroscopy of short-lived nuclei, their results regarding nuclear moments and isotopic shift, and their relation to the work of Professor K. Sugimoto.

Nuclear and High Energy PhysicsIsotopic shiftChemistryInstrumental chemistryPhysical and Theoretical ChemistryAtomic physicsTime-resolved spectroscopyCondensed Matter PhysicsCoherent spectroscopySpectroscopyAtomic and Molecular Physics and OpticsHyperfine Interactions
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Laser spectroscopy of radioactive isotopes

1987

The experimental conditions for laser spectroscopy of shortlived isotopes is discussed with respect to nuclear lifetime, reaction rates and samples preparation by on-line mass-separator techniques. The method of collinear laser spectroscopy is presented with results for medium mass elements near the closed proton shell Z=50. An interpretation of magnetic moments, spectroscopic quadrupole moments and the parabolic shape of the isotope shift in this region of nuclei is given.

Nuclear and High Energy PhysicsMagnetic momentIsotopeProtonChemistryNuclear TheoryCondensed Matter PhysicsAtomic and Molecular Physics and OpticsReaction rateQuadrupoleSample preparationPhysics::Atomic PhysicsPhysical and Theoretical ChemistryTime-resolved spectroscopyAtomic physicsNuclear ExperimentSpectroscopyHyperfine Interactions
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Time-resolved spectroscopy of ZnWO4

2001

The luminescence spectra of ZnWO 4 excited by a pulsed electron beam, pulsed nitrogen laser and X-ray are studied. The luminescence decay kinetics are compared with transient absorption relaxation kinetics. Broad band transient absorption spectra are observed and it is shown that the slow decay component (∼14 μs at RT) of the transient absorption matches well with the luminescence decay therefore this absorption is due to the excited state absorption of the luminescence center.

Nuclear and High Energy PhysicsRadiationAbsorption spectroscopyCondensed Matter::OtherChemistryRelaxation (NMR)Physics::OpticsCondensed Matter PhysicsExcited stateUltrafast laser spectroscopyGeneral Materials ScienceNitrogen laserAtomic physicsTime-resolved spectroscopyAbsorption (electromagnetic radiation)LuminescenceRadiation Effects and Defects in Solids
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Observing heme doming in myoglobin with femtosecond X-ray absorption spectroscopy.

2015

International audience; We report time-resolved X-ray absorption measurements after photolysis of carbonmonoxy myoglobin performed at the LCLS X-ray free electron laser with nearly 100 fs (FWHM) time resolution. Data at the Fe K-edge reveal that the photoinduced structural changes at the heme occur in two steps, with a faster (∼70 fs) relaxation preceding a slower (∼400 fs) one. We tentatively attribute the first relaxation to a structural rearrangement induced by photolysis involving essentially only the heme chromophore and the second relaxation to a residual Fe motion out of the heme plane that is coupled to the displacement of myoglobin F-helix

PhotodissociationAbsorption spectroscopyTime resolved spectroscopyInvited ArticlesPhotochemistrySPECIAL TOPIC: BIOLOGY WITH X-RAY LASERS 2chemistry.chemical_compoundX-ray absorption spectralcsh:QD901-999X-ray absorption near edge structureSpectroscopyInstrumentationHemeSpectroscopy[PHYS]Physics [physics]RadiationX-ray optics[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry Molecular Biology/Structural Biology [q-bio.BM]ChemistryPhotodissociationRelaxation (NMR)ChromophoreCondensed Matter PhysicsSettore FIS/07 - Fisica Applicata(Beni Culturali Ambientali Biol.e Medicin)MyoglobinBiofisica Dinamica delle proteine Spettroscopia risolta in tempo X-ray free-electron laser Assorbimento di raggi Xlcsh:CrystallographyTime-resolved spectroscopyStructural dynamics (Melville, N.Y.)
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Luminescence features of nonbridging oxygen hole centres in silica probed by site-selective excitation with tunable laser

2008

Time-resolved photoluminescence at 1.9 eV associated with the nonbridging oxygen hole centre (NBOHC) in silica was investigated under excitation with a ns pulsed laser system, tunable in the visible range. Mapping of the excitation/emission pattern evidences the site-selective excitation of the resonant zero phonon line (ZPL) transition due to its weak coupling with the stretching mode of dangling oxygen. Decay of ZPL follows an exponential law with lifetime of 15.3 μs, which provides a precise measure of the electronic transition probability of a single NBOHC.

PhotoluminescenceChemistryPhononDangling bondGeneral ChemistryCondensed Matter PhysicsMolecular electronic transitionMolecular vibrationMaterials ChemistryAtomic physicsLuminescenceInsulator Point defects luminescence time-resolved spectroscopiesExcitationTunable laser
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Excitonic luminescence in ZnO nanopowders and ceramics

2009

Abstract Fast photoluminescence spectra in the spectral region of 3.1–3.45 eV in ZnO and ZnO:Al ceramics were studied at 14 and 300 K. Ceramics with grains smaller than 100 nm were sintered from nanopowders by high pressure (8 GPa) and low temperature (350 °C). Ceramics with grain sizes 1–5 μm were sintered at 1400 °C. It is shown that excitonic luminescence spectra depend on the ceramics grain size, post preparing annealing and doping. The excitonic luminescence decay time was faster than 2 ns and the afterglow at 30 ns was ∼0.05%.

PhotoluminescenceMaterials scienceAnnealing (metallurgy)Organic ChemistryDopingAnalytical chemistryMineralogyAtomic and Molecular Physics and OpticsGrain sizeElectronic Optical and Magnetic MaterialsAfterglowInorganic Chemistryvisual_artvisual_art.visual_art_mediumCeramicElectrical and Electronic EngineeringPhysical and Theoretical ChemistryTime-resolved spectroscopyLuminescenceSpectroscopyOptical Materials
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Photoluminescence of Carbon Dots Embedded in a SiO2 Matrix

2016

Abstract We synthetized carbon dots by a pyrolitic method, and studied their photoluminescence in aqueous environment and upon trapping in a solid matrix. To this aim, we devised a facile procedure allowing to embed the dots in amorphous SiO2, without the need of any pre-functionalization of the nanoparticles, and capable of yielding a brightly photoluminescent monolith. Experimental data reveal a remarkable similarity between the emission properties of carbon dots in water and in SiO2, suggesting that the chromophores responsible of the photoluminescence undergo only weak interactions with the environment. Time-resolved photoluminescence data reveal that the typical photoluminescence tunab…

PhotoluminescenceMaterials sciencePhotoluminescent nanocarbonAnalytical chemistryNanoparticlechemistry.chemical_element02 engineering and technologyTrapping010402 general chemistry01 natural sciencesCarbon dots; Photoluminescent nanocarbons; Photoluminescent silica monolith; Time-resolved photoluminescence; Materials Science (all)Photoluminescence excitationMonolithgeographygeography.geographical_feature_categorybusiness.industryChromophore021001 nanoscience & nanotechnologyCarbon dot0104 chemical sciencesAmorphous solidchemistryOptoelectronicsPhotoluminescent silica monolithMaterials Science (all)0210 nano-technologybusinessCarbonTime-resolved photoluminescence
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Homogeneous and inhomogeneous contributions to the luminescence linewidth of point defects in amorphous solids: Quantitative assessment based on time…

2008

The article describes an experimental method that allows to estimate the inhomogeneous and homogeneous linewidths of the photoluminescence band of a point defect in an amorphous solid. We performed low temperature time-resolved luminescence measurements on two defects chosen as model systems for our analysis: extrinsic Oxygen Deficient Centers (ODC(II)) in amorphous silica and F+ 3 centers in crystalline Lithium Fluoride. Measurements evidence that only defects embedded in the amorphous matrix feature a dependence of the radiative decay lifetime on the emission energy and a time dependence of the first moment of the emission band. A theoretical model is developed to link these properties to…

PhotoluminescenceMaterials sciencebusiness.industryLithium fluorideFOS: Physical sciencessilica time-resolved luminescence spectral widthDisordered Systems and Neural Networks (cond-mat.dis-nn)Condensed Matter - Disordered Systems and Neural NetworksCondensed Matter PhysicsMolecular physicsCrystallographic defectElectronic Optical and Magnetic MaterialsAmorphous solidLaser linewidthchemistry.chemical_compoundCondensed Matter::Materials ScienceOpticschemistrybusinessLuminescenceSpectroscopyEnergy (signal processing)
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