Search results for "ToF"

Proteomic analysis of exosome-like vesicles derived from breast cancer cells.

2012

Background/Aim: The phenomenon of membrane vesicle-release by neoplastic cells is a growing field of interest in cancer research, due to their potential role in carrying a large array of tumor antigens when secreted into the extracellular medium. In particular, experimental evidence show that at least some of the tumor markers detected in the blood circulation of mammary carcinoma patients are carried by membrane-bound vesicles. Thus, biomarker research in breast cancer can gain great benefits from vesicle characterization. Materials and Methods: Conditioned medium was collected from serum starved MDA-MB-231 sub-confluent cell cultures and exosome-like vesicles (ELVs) were isolated by ultra…

Domain Generation Algorithm Detection Using Machine Learning Methods

2018

A botnet is a network of private computers infected with malicious software and controlled as a group without the knowledge of the owners. Botnets are used by cybercriminals for various malicious activities, such as stealing sensitive data, sending spam, launching Distributed Denial of Service (DDoS) attacks, etc. A Command and Control (C&C) server sends commands to the compromised hosts to execute those malicious activities. In order to avoid detection, recent botnets such as Conficker, Zeus, and Cryptolocker apply a technique called Domain-Fluxing or Domain Name Generation Algorithms (DGA), in which the infected bot periodically generates and tries to resolve a large number of pseudorando…

Reply to the Letter Entitled "FeNO Variability when Using Different Analyzers at the Joint ATS/ERS Guideline Cutoff".

2019

Estudio de perfilación de metabolitos secundarios de extractos vegetales obtenidos mediante técnicas y estrategias no convencionales para el desarrol…

2022

En esta tesis doctoral se desarrolló en el entorno de la economía verde y el reciclaje de residuos alimentarios estudiando diferentes técnicas de extracción no convencionales. En primer lugar, se aislaron polifenoles de matrices complejas producto del desperdicio de alimentos, como son las hojas de Moringa oleifera, mediante extracción asistida por ultrasonidos (UAE). El enfoque de diseño experimental permitió optimizar las mejores condiciones de extracción (MeOH:H2O 50:50, v/v, proporcion 1:60 Sólido/Líquido (S/L), 60°C, 60 min) para obtener un contenido fenólico total (TPC) de 13,4 mg expresados como equivalentes de ácido gálico (GAE)/g de materia seca. Por lo tanto, la caracterización y …

On the Pauli-Villars regularisation scheme in the NJL model

1993

Abstract We consider the Nambu-Jona-Lasinio model with a Pauli-Villars cutoff. We point out that previous attempts to use this regularisation have not been consistent and it is shown that if used correctly this approach yields very reasonable values for the cutoff parameter and quark condensate when we demand that ƒπ is fixed to its experimental value. Finally it is argued that because of its covariance and smooth nature this regularisation scheme has significant advantages over other regulators on the market.

Dijets at Tevatron Cannot Constrain SMEFT Four-Quark Operators

2019

We explore the sensitivity of Tevatron data to heavy new physics effects in differential dijet production rates using the SMEFT in light of the fact that consistent and conservative constraints from the LHC cannot cover relatively low cutoff scales in the EFT. In contrast to the results quoted by the experimental collaborations and other groups, we find that, once consistency of the perturbation expansion is enforced and reasonable estimates of theoretical errors induced by the SMEFT series in $\frac{E}{\Lambda}$ are included, there is no potential to constrain four-quark contact interactions using Tevatron data. This shows the general difficulty of constraining physics model-independently …

Rhema ghennàion. Responsabilità ‘genetica’ del poeta tragico nelle Rane di Aristofane

2017

Questo contributo si propone di indagare il rapporto tra cultura e politica nelle Rane di Aristofane attraverso una delineazione delle nozioni di responsabilità e merito nella figura del poeta tragico au-spicata da Aristofane. Dall’appartenenza del poeta alle Muse, che si configura nella sua partecipa-zione ai loro Misteri, discende una sua responsabilità ‘genetica’: il poeta utile alla polis è infatti fer-tile (γόνιμος), ovvero, è in grado di fecondare il teatro, e quindi la città, di valori che, ispirandosi agli insegnamenti del passato, educhino i cittadini. Intorno al concetto di ‘nobiltà’ (γενναιότης), unito a quello di fecondità (sulla base della radice *γεν-), si sviluppa l’idea di u…

Hydroquinone: O-glucosyltransferase from cultivated Rauvolfia cells: enrichment and partial amino acid sequences.

2000

Plant cell suspension cultures of Rauvolfia are able to produce a high amount of arbutin by glucosylation of exogenously added hydroquinone. A four step purification procedure using anion exchange, hydrophobic interaction, hydroxyapatite-chromatography and chromatofocusing delivered in a yield of 0.5%, an approximately 390 fold enrichment of the involved glucosyltransferase. SDS-PAGE showed a M(r) for the enzyme of 52 kDa. Proteolysis of the pure enzyme with endoproteinase LysC revealed six peptide fragments with 9-23 amino acids which were sequenced. Sequence alignment of the six peptides showed high homologies to glycosyltransferases from other higher plants.

Fine analysis of the chromatin structure of the yeast SUC2 gene and of its changes upon derepression. Comparison between the chromosomal and plasmid-…

1987

Micrococcal nuclease digestion has been used to investigate some fine details of the chromatin structure of the yeast SUC2 gene for invertase. Precisely positioned nucleosomes have been found on a 2 kb sequence from the 3' non-coding region, and four nucleosomes also seem to occupy fixed positions on the 5' flank. Eleven nucleosomes lie on the coding region, although their positioning is not as precise as in the flanks. When the gene is derepressed, these latter nucleosomes adopt a more open conformation and so do two of the nucleosomes positioned on the 5' flank. A dramatic change occurs in the 3' flank, whose involvement in the structural transitions of chromatin upon gene activation is p…

Molecular mode-coupling theory applied to a liquid of diatomic molecules

2000

We study the molecular mode coupling theory for a liquid of diatomic molecules. The equations for the critical tensorial nonergodicity parameters ${\bf F}_{ll'}^m(q)$ and the critical amplitudes of the $\beta$ - relaxation ${\bf H}_{ll'}^m(q)$ are solved up to a cut off $l_{co}$ = 2 without any further approximations. Here $l,m$ are indices of spherical harmonics. Contrary to previous studies, where additional approximations were applied, we find in agreement with simulations, that all molecular degrees of freedom vitrify at a single temperature $T_c$. The theoretical results for the non ergodicity parameters and the critical amplitudes are compared with those from simulations. The qualitat…