Search results for "ToP"
showing 10 items of 17685 documents
Role of hematopoietic stem and progenitor cells in training immunity against infection
2022
During an infection, haematopoiesis is altered to increase the output of mature myeloid cells to fight off the pathogen. It has been demonstrated that the detection of pattern recognition receptor agonists by hematopoietic stem and progenitor cells (HSPCs) induces their differentiation towards mature myeloid cells with modified phenotypes. In the present PhD thesis, we show that an in vitro transient exposure of HSPCs to live Candida albicans cells is sufficient to induce a trained phenotype of the macrophages they produce in a dectin-1- and TLR2-dependent manner. Additionally, we use an HSPC transplantation mouse model to demonstrate that the direct interaction of β-glucans and their recep…
CONSORT compliance in randomized surgical trials assessing the analgesic and anti-inflammatory effectiveness of preoperative drug management of patie…
2022
Investigate methodological quality of clinical trials in mandibular third molar surgery and its compliance with the consort statement. An electronic search was performed in five journal websites, chose the five scientific journals with the greatest impact factor in oral and maxillofacial surgery according to the SCImago Journal Rank. The compliance of studies with the CONSORT statement was assessed. Also, the risk of bias of each study was evaluated. Twenty-nine studies were included. The average CONSORT compliance score was 25.50 (79.68%). Most studies were performed in the Americas (n = 14, 48.3%) and Asia (n = 10, 34.5%). Parallel-group (n=15, 51.7%) and split-mouth RCTs (n=11, 38%) were…
The swinholide biosynthetic gene cluster from a terrestrial cyanobacterium Nostoc sp. UHCC 0450
2018
Swinholides are 42-carbon ring polyketides with a 2-fold axis of symmetry. They are potent cytotoxins that disrupt the actin cytoskeleton. Swinholides were discovered from the marine sponge Theonella sp. and were long suspected to be produced by symbiotic bacteria. Misakinolide, a structural variant of swinholide, was recently demonstrated to be the product of a symbiotic heterotrophic proteobacterium. Here, we report the production of swinholide A by an axenic strain of the terrestrial cyanobacterium Nostoc sp. strain UHCC 0450. We located the 85-kb trans-AT polyketide synthase (PKS) swinholide biosynthesis gene cluster from a draft genome of Nostoc sp. UHCC 0450. The swinholide and misaki…
Estudio de la incompatibilidad ABO en el trasplante alogénico de progenitores hematopoyéticos
2021
La incompatibilidad ABO entre donante y receptor no supone un impedimento en la realización de un alotrasplante de progenitores hematopoyéticos (alo-TPH). Sin embargo, se ha asociado con una mayor morbilidad y mortalidad, así como a un aumento de eventos inmunohematológicos y de requerimientos transfusionales en los pacientes trasplantados con incompatibilidad ABO. Planteamos la hipótesis de que un conocimiento detallado de estos aspectos podría ser crucial para optimizar el manejo y la estrategia transfusional en el paciente individual sometido a un trasplante con incompatibilidad ABO y así disminuir la morbilidad y mortalidad asociada. El objetivo general de la tesis es realizar un anális…
Torque teno virus como marcador subrogado de inmunocompetencia en el trasplante
2019
El torque teno virus (TTV), es un virus de pequeño tamaño de ADN de cadena sencilla, desnudo y capsulado perteneciente a la familia Anelloviridae con una prevalencia aproximada del 95% independiente del sexo y del estado socioeconómico y cuya carga plasmática está relacionada con la funcionalidad del sistema inmune y por tanto puede ser útil para evaluar el riesgo de eventos adversos relacionados con la inmunosupresión. El efecto del acondicionamiento sobre la ADNemia de TTV en pacientes sometidos a trasplante alogénico de precursores hematopoyéticos (alo-TPH) ocasiona un descenso de las cargas del virus que se ve acompañado por un descenso paralelo del recuento absoluto de linfocitos. Tras…
Parámetros biológicos predictivos de la infección activa precoz por Citomegalovirus en el trasplante alogénico de precursores hematopoyéticos
2021
El trasplante alogénico de células precursoras hematopoyéticas es la única solución curativa para un variado número de neoplasias hematológicas de alto riego. Por esta razón, la infección sistémica causada por CMV ocasiona frecuente morbilidad y mortalidad en el paciente receptor de este tipo de trasplantes. En la actualidad se emplean dos estrategias terapéuticas. Sin embargo, ninguna está exenta de riesgos y ambas son mejorables. Disponer de marcadores biológicos que permitan estratificar a los pacientes de acuerdo al riesgo de desarrollo de infección sistémica haría posible una profilaxis dirigida, lo que resultaría en una menor toxicidad para el paciente y en menor incidencia de complic…
Impacto del quimerismo hematopoyético precoz en el injerto tras trasplante alogénico de progenitores de sangre de cordón umbilical de donante no empa…
2013
El trasplante de sangre de cordón umbilical (TSCU) ha experimentado un auge muy importante en los últimos años como alternativa a la médula ósea y sangre periférica, debido a la dificultad de encontrar donantes altruistas de estas fuentes de progenitores hematopoyéticos. Los datos clínicos disponibles hasta la fecha muestran una supervivencia libre de enfermedad similar a la observada con el trasplante de médula ósea (TMO) o sangre periférica (TSP). Sin embargo, como consecuencia de la baja dosis celular contenida en la SCU, la tasa de fallo de injertos es más elevada y el tiempo hasta la recuperación de la neutropenia más prolongado. Gran parte de la investigación en el terreno del TSCU se…
An Overview on the Use of miRNAs as Possible Forensic Biomarkers for the Diagnosis of Traumatic Brain Injury
2023
Determining the cause of death is one of the main goals of forensic pathology. However, conditions can occur in which common approaches—external inspection, autopsy, histology, etc.—might not be conclusive. With the advancement of molecular biology, several investigative techniques have been developed over the years, and the application as approaches complementary to routine procedures has proved useful in these cases. In this context, microRNA (miRNA) profiling has attracted increasing interest due to these molecules’ ability to regulate physiological and pathological processes. The evidence of differential miRNA expression in both animal models and human samples of traumatic brain injury …
The Impact of a 1,2,3-Triazole Motif on the Photophysical Behavior of Non-K Tetrasubstituted Pyrene with a Substitution Pattern Providing the Long Ax…
2022
1,3,6,8-Tetrasubstituted pyrene derivatives with two types of substituents (4-(2,2-dimethylpropyloxy)pyridine, 1-decyl-1,2,3-triazole, 1-benzyl-1,2,3-triazole, and pyrazole), substituted in such a way that provides the long axial symmetry, are prepared and characterized in the present study. To the best of our knowledge, the pyrene derivative containing the same heteroaryl motif (triazole) but substituted by two various alkyls, straight decyl and benzyl-based side chains (C), is reported for the first time. For comparison, compounds with one kind of triazole motif and substituted pyridine or pyrazole groups were prepared (A and B). The photophysical properties of all molecules were evaluate…
Hydrogen isotopes (δ2H) of polyunsaturated fatty acids track bioconversion by zooplankton
2022
1. Organisms at the base of aquatic food webs synthesize essential nutrients, such as omega-3 polyunsaturated fatty acids (n-3 PUFA), which are transferred to consumers at higher trophic levels. Many consumers, requiring n-3 long-chain (LC) PUFA, such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), have limited ability to biosynthesize them from the essential dietary precursor α-linolenic acid (ALA) and thus rely on dietary provision of LC-PUFA. 2. We investigated LC-PUFA metabolism in freshwater zooplankton using stable hydrogen isotopes (δ2H) of fatty acids as tracers. We conducted feeding experiments with the freshwater keystone grazer Daphnia to quantify changes in the δ2…