Search results for "Toxoplasma gondii"
showing 7 items of 27 documents
Quantification and viability assays of Toxoplasma gondii in commercial "Serrano" ham samples using magnetic capture real-time qPCR and bioassay techn…
2014
"Serrano" ham is a typical pork product from the Mediterranean area, highly valued for its flavour. To make Serrano ham, pork undergoes a salting and a subsequent fermentation process known as curing. Certain pigs used for meat production are an important source of Toxoplasma gondii infection in humans. We have developed a method for quantifying and assaying the viability of the T. gondii present in commercial Serrano ham samples. A magnetic capture method for the isolation of T. gondii DNA and a qRT-PCR were used to estimate the T. gondii burden in 475 commercial samples of "Serrano" ham in two presentation formats: ham pieces and sliced ham. The infectivity capacity of T. gondii in positi…
Absence of Toxoplasma gondii in 100% Iberian products from experimentally infected pigs cured following a specific traditional process.
2020
Infection with Toxoplasma gondii in humans has usually been related to the consumption of raw, undercooked or cured meat. Our study is based on the detection of T. gondii in cured legs and shoulders made from 100% Iberian sows fed mainly with acorn and raised as outdoor livestock in Aracena (Spain), which having been elaborated following a specific curing process (time period and location). An outdoor farm with a total of 636 animals was studied, showing a seroprevalence of 10% for the parasite T. gondii. Twenty individuals were chosen to be experimentally infected and slaughtered 60 days post-infection. Their legs and shoulders were processed to make 100% Iberian ham legs and shoulders. Th…
A High Sensitive Nested PCR for Toxoplasma gondii Detection in Animal and Food Samples
2013
Toxoplasma gondii is a major food and waterborne transmitted parasite world-wide. The tissues and meat samples of many warm blooded animals can contain tissues cysts from chronic toxoplasmosis. Water and vegetable can be contaminated by the parasitic oocysts shed through the feces of infected cats, representing the definitive host of the parasite. A sensitive PCR for Toxoplasma gondii detection is described. The first step amplified the region between the 28S and 18S rDNA in the closely related T. gondii and Neospora caninum; RFLP analysis distinguished the DNA from the two morphologically identical parasites. Although N. caninum is not involved in human transmission, so far, it is importan…
Pityriasis lichenoides chronica associated with herpes simplex virus type 2.
2012
Introduction. Pityriasis lichenoides is a rare, acquired spectrum of skin conditions of an unknown etiology.Case Report. A 28-year-old man presented with recurrent outbreaks of herpes simplex virus associated with the onset of red-to-brown maculopapules located predominantly in trunk in each recurrence. Positive serologies to herpes simplex virus type 2 were detected. Histopathological examination of one of the lesions was consistent with a diagnosis of pityriasis lichenoides chronica.Discussion. Pityriasis lichenoides is a rare cutaneous entity of an unknown cause which includes different clinical presentations. A number of infectious agents have been implicated based on the clustering of …
Investigation and management of Toxoplasma gondii infection in pregnancy and infancy: a prospective study.
2011
Aim:Toxoplasma gondii infection during pregnancy poses a serious risk to the fetus, therefore timely and accurate diagnosis is essential. The aim of this study was to estimate the frequency of congenital infection via evaluating mother's immunological status and the possibility to improving the diagnostic and therapeutic approaches.Methods:Eighty five mothers with Toxoplasma seroconversion and their offspring were enrolled (among them, 2 spontaneous abortions were documented in the first trimester). Prenatal PCR diagnosis was carried out on 50 patients (60%), with 7 positive cases (14%). Morphological ultrasound scanning revealed anomalies in one fetus. Long-term follow-up included general …
Follow-up post natale in nati da madre con infezione certa da Toxoplasma gondii considerazioni sul management prenatale.
2005
The clinical management of perinatal toxoplasmosis involves a gynaecologist during pregnancy and a neonatologist after delivery. Then, in the absence of a uniform approach, early evaluation of infected infants requires a thorough long-term follow-up also in asymptomatic children, who have to be observed for at least one year due to unpredictable sequelae in later life. We retrospectively analyzed pregnancy management of 54 women with certain infection from Toxoplasma gondii (TG) and prospectively enrolled their infants to compare prenatal management with postnatal clinical outcome. All mothers with seroconversion for TG infection were from the Palermo area and were retrospectively analyzed,…
Vienšūnas parazīta Toxoplasma gondii sastopamība meža cūkās (Sus scrofa)
2021
Termiski nepietiekoši apstrādātas meža cūkas gaļas lietošana uzturā ir potenciāls Toxoplasma gondii invāzijas avots cilvēkiem. Zināšanas par T. gondii izplatību un sastopamību meža cūkās, dod iespēju apzināt riskus cilvēku veselībai un izstrādāt profilakses pasākumus stratēģijas. Pētījuma mērķis bija noteikt T. gondii sastopamību meža cūkās Latvijā un sakarības ciešumu starp T. gondii DNS un specifisko pret-T. gondii IgG antivielu klātbūtni meža cūkās. Pētījuma ietvaros tika izmantota seroloģiskā metode (ELISA) un divas T. gondii DNS izdalīšanas metodes – no hidrolīzes koncentrāta kopējā DNS izdalīšana un ar magnētiskām lodītēm specifiskā T. gondii DNS izdalīšana. Pēc sistemātiskās literatū…