Search results for "Trace"

showing 10 items of 3218 documents

Elicitins, proteinaceous elicitors of plant defense, are a new class of sterol carrier proteins

1998

Some phytopathogenic fungi within Phytophthora species are unable to synthesize sterols and therefore must pick them up from the membranes of their host-plant, using an unknown mechanism. These pseudo-fungi secrete elicitins which are small hydrophilic cystein-rich proteins. The results show that elicitins studied interact with dehydroergosterol in the same way, but with some time-dependent differences. Elicitins have one binding site with a similar strong affinity for dehydroergosterol. Using a non-steroid hydrophobic fluorescent probe, we showed that phytosterols are able to similarly bind to elicitins. Moreover, elicitins catalyze sterol transfer between phospholipidic artificial membran…

0106 biological sciencesPhytophthora[SDV]Life Sciences [q-bio]Biophysics01 natural sciencesBiochemistryFungal Proteins03 medical and health sciencesNaphthalenesulfonatesErgosterolPlant defense against herbivoryExtracellularSecretionBinding sitePERSPECTIVEMolecular BiologyPhospholipidsComputingMilieux_MISCELLANEOUS030304 developmental biologyFluorescent Dyes0303 health sciencesBinding SitesbiologyfungiAlgal ProteinsPhytosterolsElicitinBiological TransportCell BiologyPlantsbiology.organism_classificationSterolCell biology[SDV] Life Sciences [q-bio]KineticsMembraneSpectrometry FluorescenceBiochemistryPhytophthoraCarrier Proteins010606 plant biology & botanyProtein Binding
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The skeletal proteome of the coral Acropora millepora: the evolution of calcification by co-option and domain shuffling.

2013

14 pages; International audience; In corals, biocalcification is a major function that may be drastically affected by ocean acidification (OA). Scleractinian corals grow by building up aragonitic exoskeletons that provide support and protection for soft tissues. Although this process has been extensively studied, the molecular basis of biocalcification is poorly understood. Notably lacking is a comprehensive catalog of the skeleton-occluded proteins-the skeletal organic matrix proteins (SOMPs) that are thought to regulate the mineral deposition. Using a combination of proteomics and transcriptomics, we report the first survey of such proteins in the staghorn coral Acropora millepora. The or…

0106 biological sciencesProteomeCoralMolecular Sequence Datacalcium carbonate skeletonProteomics010603 evolutionary biology01 natural sciencesMass SpectrometryCalcium CarbonateEvolution Molecular03 medical and health sciencesAcropora milleporaCalcification PhysiologicproteomicsPhylogeneticsAnthozoa[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]evolutionGeneticsAnimals14. Life underwaterAmino Acid Sequencescleractinian[SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/BiomaterialsMolecular BiologyEcology Evolution Behavior and SystematicsDiscoveriesPhylogeny030304 developmental biologyStaghorn coral0303 health sciencesbiologySequence Homology Amino AcidEcologyMolecular Sequence Annotationbiology.organism_classification[ SDV.IB.BIO ] Life Sciences [q-bio]/Bioengineering/BiomaterialsAnthozoabiomineralizationExtracellular MatrixProtein Structure TertiaryEvolutionary biology[ SDV.BBM.GTP ] Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]ProteomeSequence AlignmentFunction (biology)
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Nitric oxide signalling in plants: interplays with Ca2+ and protein kinase

2008

International audience; Much attention has been paid to nitric oxide (NO)research since its discovery as a physiological mediator of plant defence responses. In recent years, newer roles have been attributed to NO, ranging from root development to stomatal closure. The molecular mechanisms underlying NO action in plants are just begun to emerge. The currently available data illustrate that NO can directly influence the activity of target proteins through nitrosylation and has the capacity to act as a Ca2+-mobilizing intracellular messenger. The interplay between NO and Ca2+ has important functional implications, expanding and enriching the possibilities for modulating transduction processes…

0106 biological sciencesSIGNALLINGPhysiologyPlant ScienceBiology01 natural sciencesNitric oxide03 medical and health sciencesTransduction (genetics)chemistry.chemical_compoundSNF-RELATED PROTEIN KINASE 2Mediator030304 developmental biology0303 health sciencesADP-RIBOSE CYCLIQUEPROTEIN KINASESKinaseCALCIUM 2+NitrosylationPlants[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/BotanicsNitric oxide metabolismCell biologySignallingBiochemistrychemistryCalciumIntracellularNITRIC OXIDE010606 plant biology & botanySignal Transduction
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Evaluation and comparison of trace metal accumulation in different tissues of potential bioindicator organisms: Macrobenthic filter feedersStyela pli…

2016

Trace metal concentrations were measured in different tissues of Sabella spallanzanii, Styela plicata, and Mytilus galloprovincialis collected in the Termini Imerese Harbor (Sicily, Italy) to evaluate the potential use of these species as bioindicators. Higher bioaccumulation factors (BAFs) were calculated in the tube of S. spallanzanii, except for As, which had a higher BAF in the branchial crown of the same species. Regarding the other species analyzed, higher BAFs were found in the digestive gland of M. galloprovincialis. An exception was Pb, which was significantly more concentrated in the branchial basket and tunic of S. plicata. The BAFs calculated in the present study show that all t…

0106 biological sciencesSabella spallanzaniibiologyEcology010604 marine biology & hydrobiologyHealth Toxicology and MutagenesisZoology010501 environmental sciencesbiology.organism_classification01 natural sciencesMytilusFilter (aquarium)Styela plicataFilter feedingBioaccumulationEnvironmental ChemistryTrace metalBioindicator0105 earth and related environmental sciencesEnvironmental Toxicology and Chemistry
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Distribution of sea urchins living near shallow water CO2 vents is dependent upon species acid-base and ion-regulatory abilities.

2013

To reduce the negative effect of climate change on Biodiversity, the use of geological CO2 sequestration has been proposed; however leakage from underwater storages may represent a risk to marine life. As extracellular homeostasis is important in determining species' ability to cope with elevated CO2, we investigated the acid-base and ion regulatory responses, as well as the density, of sea urchins living around CO2 vents at Vulcano, Italy. We conducted in situ transplantation and field-based laboratory exposures to different pCO2/pH regimes. Our results confirm that sea urchins have some ability to regulate their extracellular fluid under elevated pCO2. Furthermore, we show that even in cl…

0106 biological sciencesSettore BIO/07 - EcologiaGeological Phenomena010504 meteorology & atmospheric sciencesClimate ChangeSpecies distributionBiodiversityMarine lifeAquatic ScienceOceanography01 natural sciencesParacentrotus lividushowever leakage from underwater storages may represent a risk to marine life. As extracellular homeostasis is important in determining species' ability to cope with elevated CO2 we investigated the acid-base and ion regulatory responses as well as the density of sea urchins living around CO2 vents at Vulcano Italy. We conducted in situ transplantation and field-based laboratory exposures to different pCO2/pH regimes. Our results confirm that sea urchins have some ability to regulate their extracellular fluid under elevated pCO2. Furthermore we show that even in closely-related taxa divergent physiological capabilities underlie differences in taxa distribution around the CO2 vent. It is concluded that species distribution under the sort of elevated CO2 conditions occurring with leakages from geological storages and future ocean acidification scenarios may partly be determined by quite subtle physiological differentiation.Mediterranean seaBenthosAnimalsSeawater14. Life underwaterEcosystem0105 earth and related environmental sciencesbiologyEcology010604 marine biology & hydrobiologyTo reduce the negative effect of climate change on Biodiversity the use of geological CO2 sequestration has been proposedOcean acidificationCarbon DioxideHydrogen-Ion Concentrationbiology.organism_classificationAdaptation PhysiologicalPollutionTransplantationOceanographyItaly13. Climate actionSea UrchinsWater Pollutants Chemical
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The plasma membrane oxidase NtrbohD is responsible for AOS production in elicited tobacco cells

2002

Summary A cDNA encoding a protein, NtrbohD, located on the plasma membrane and homologue to the flavocytochrome of the neutrophil NADPH oxidase, was cloned in tobacco. The corresponding mRNA was accumulated when tobacco leaves and cells were treated with the fungal elicitor cryptogein. After elicitation with cryptogein, tobacco cells transformed with antisense constructs of NtrbohD showed the same extracellular alkalinization as the control, but no longer produced active oxygen species (AOS). This work represents the first demonstration of the function of a homologue of gp91–phox in AOS production in elicited tobacco cells.

0106 biological sciencesTime FactorsNicotiana tabacumMolecular Sequence DataPlant ScienceBiologyGenes Plant01 natural sciencesFungal Proteins[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics03 medical and health sciences[SDV.GEN.GPL] Life Sciences [q-bio]/Genetics/Plants geneticsComplementary DNATobaccoGene expressionGeneticsExtracellularAOSAmino Acid SequenceRNA MessengerCells CulturedComputingMilieux_MISCELLANEOUS030304 developmental biology0303 health sciencesOxidase testNADPH oxidaseGene Expression ProfilingAlgal ProteinsCell MembraneHydrogen PeroxideCell BiologyHydrogen-Ion ConcentrationPlants Genetically Modifiedbiology.organism_classification3. Good healthElicitorCell biologyPlant LeavesProtein TransportBiochemistryCell culturebiology.proteinOxidoreductasesReactive Oxygen Species010606 plant biology & botany
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Trace element storage capacity of sediments in deadPosidonia oceanicamat from a chronically contaminated marine ecosystem

2016

Posidonia oceanica mat is considered a long-term bioindicator of contamination. Storage and sequestration of trace elements and organic carbon (Corg) were assessed in dead P. oceanica mat and bare sediments from a highly polluted coastal marine area (Augusta Bay, central Mediterranean). Sediment elemental composition and sources of organic matter have been altered since the 1950s. Dead P. oceanica mat displayed a greater ability to bury and store trace elements and Corg than nearby bare sediments, acting as a long-term contaminant sink over the past 120?yr. Trace elements, probably associated with the mineral fraction, were stabilized and trapped despite die-off of the overlying P. oceanica…

0106 biological sciencesTotal organic carbonchemistry.chemical_classificationbiologyEcology010604 marine biology & hydrobiologyHealth Toxicology and MutagenesisTrace elementSediment010501 environmental sciencesbiology.organism_classification01 natural sciencesDredgingchemistryPosidonia oceanicaEnvironmental chemistryEnvironmental ChemistryEnvironmental scienceOrganic matterBioindicatorBay0105 earth and related environmental sciencesEnvironmental Toxicology and Chemistry
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Reducing waste and ecological impacts through a sustainable and efficient management of perishable food based on the Monte Carlo simulation

2019

Abstract In today’s competitive global market it is mandatory to improve warehousing operations integrating economic, environmental and social aspects. The recent advancement in monitoring technologies can greatly improve the performance of the food supply chain reducing product loss. In particular, in the perishable food supply chain, initially inventory operations are critical because they manage the material flows in very variable conditions. The deterioration level of the products as well as the market demand are the main factors that can influence warehouse strategy. This research aims to consider the application of sustainability principles in the context of warehouse storage, evaluat…

0106 biological sciencesTraceabilityComputer scienceSupply chainGeneral Decision SciencesContext (language use)010501 environmental sciences010603 evolutionary biology01 natural sciencesWarehouse managementSupply and demandSettore ING-IND/17 - Impianti Industriali MeccaniciMonte Carlo simulationEcology Evolution Behavior and Systematics0105 earth and related environmental sciencesEcologyEcological impacts Food waste reduction Monte Carlo simulation Shelf life model Sustainability Warehouse managementEnvironmental economicsEcological impactWarehouseProduct (business)SustainabilitySustainabilityShelf life modelCarbon footprintEcological impacts; Food waste reduction; Monte Carlo simulation; Shelf life model; Sustainability; Warehouse managementFood waste reductionEcological Indicators
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Hybrids and allied species as potential rootstocks for eggplant: Effect of grafting on vigour, yield and overall fruit quality traits

2018

Abstract Grafting of fruiting vegetables is an effective technique to overcome pests and diseases in modern cropping systems and it is often used to improve yield and fruit quality. Eggplant is an important vegetable crop that benefits significantly from grafting. In this regards, the exploitation, valorization and breeding of new rootstock genotypes as possible substitute to those commonly used (Solanum torvum and tomato hybrids) would permit an intensive eggplant crop system in those situations where a rootstock rotation is required. In the present article, we study the effects of several potential rootstocks including both wild/allied species of eggplant [S. torvum (STO), S. macrocarpon …

0106 biological sciencesbiologyCrop yieldGrowing season04 agricultural and veterinary sciencesHorticulturebiology.organism_classificationGrafting01 natural sciencesCropHorticultureAgronomyYield (wine)040103 agronomy & agricultureEggplant Solanum spp. Grafting Scion/rootstock combination Nutraceutical0401 agriculture forestry and fisheriesSolanum torvumRootstock010606 plant biology & botanyHybrid
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Infection by Endosymbiotic “Male-Killing” Bacteria in Coleoptera

2018

Wolbachia, Rickettsia, Spiroplasma and Cardinium are endosymbiotic and intracellular bacteria known to cause numerous disorders in host reproduction, reflected in their common name “male-killers”. In this study, 297 beetle species from various taxonomic groups were screened with the use of molecular markers for the presence of infection by any of these endosymbionts. Wolbachia was found to be the most common “male-killer” among beetle hosts as it infected approx. 27% of species. Rickettsia, Spiroplasma and Cardinium were much less prevalent as they infected: 8%, 3% and 2%, respectively, of the studied beetle species. This is the first report of Cardinium presence in beetle hosts. Incidences…

0106 biological sciencesbiologySpiroplasmabeetleintracellular infectionSpiroplasmaGeneral Medicinebiology.organism_classification010603 evolutionary biology01 natural sciencesGeneral Biochemistry Genetics and Molecular BiologyMicrobiology010602 entomologychemistry.chemical_compoundRickettsiachemistryMolecular markerCardiniumWolbachiaRickettsiaBacteriaEndosymbiotic bacteriaWolbachiaFolia Biologica-Krakow
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