Search results for "Transcription"

showing 10 items of 2278 documents

OPLA scaffold, collagen I, and horse serum induce a higher degree of myogenic differentiation of adult rat cardiac stem cells

2009

In the last few years, a major goal of cardiac research has been to drive stem cell differentiation to replace damaged myocardium. Several research groups have attempted to differentiate potential cardiac stem cells (CSCs) using bi- or three-dimensional systems supplemented with growth factors or molecules acting as differentiating substances. We hypothesize that these systems failed to induce a complete differentiation because they lacked an architectural space. In the present study, we isolated a pool of small proliferating and fibroblast-like cells from adult rat myocardium. The phenotype of these cells was assessed and the characterized cells were cultured in a collagen I/OPLA scaffold …

SerumScaffoldPhysiologyCellular differentiationLIM-Homeodomain ProteinsClinical BiochemistryNerve Tissue ProteinsCell SeparationBiologyMuscle DevelopmentCollagen Type INestinRats Sprague-DawleyIntermediate Filament ProteinsMicroscopy Electron TransmissionTroponin TAnimalsMyocyteMyocytes CardiacHorsesTranscription factorHomeodomain ProteinsMyosin Heavy ChainsTissue ScaffoldsSettore BIO/16 - Anatomia UmanaMyocardiumCell DifferentiationCell BiologyAnatomyNestinPhenotypestem cell OPLA scaffoldActinsIn vitroClone CellsGATA4 Transcription FactorRatsCell biologyAdult Stem CellsProto-Oncogene Proteins c-kitConnexin 43FemaleStem cellTranscription FactorsJournal of Cellular Physiology
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Polymer aging affects the bioavailability of microplastics-associated contaminants in sea urchin embryos

2022

Microplastics (MPs) in the marine environment undergo complex weathering factors that can affect their ability to interact with different coexisting environmental contaminants (termed here co-contaminants). In this study, the influence of artificially aging using UV on the sorption of a complex mixture of co-contaminants onto MPs was investigated in order to provide meaningful hypotheses on their individual and combined toxicities on sea urchin embryos. A mixture of artificially aged MPs (PS particles and PA microfibers) combined with 2,2′ ,4,4′ - tetrabromodiphenyl ether (BDE-47), or Cd or Cu, both alone and in a mix, were used to expose embryos of Paracentrotus lividus. The effects of pol…

Settore BIO/07 - EcologiaEnvironmental EngineeringPolymersMicroplasticsHealth Toxicology and MutagenesisPublic Health Environmental and Occupational HealthBiological AvailabilitySettore BIO/11 - Biologia MolecolareGeneral MedicineGeneral ChemistryComplex MixturesPollutionSettore CHIM/03 - Chimica Generale E InorganicaParacentrotusMicroplastics Transcriptional profiling Metal mixture Artificial aging Sea urchin PBDEAnimalsEnvironmental ChemistryPlasticsCadmiumChemosphere
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H1° mRNA-containing complexes in rat brain cells. In: Proceedings of the Abstracts

2015

Post-transcriptional regulation of gene expression depends on RNA-binding proteins (RBPs), which are able to regulate translation, stability and subcellular localization of mRNAs [1]. RNA-protein complexes start to be built up since transcription; some proteins remain then bound to the transcript, while others behave as only transient components. In the developing nervous system of mammals, the postnatal production of the histone variants H1° and H3.3 is mainly regulated at the post-transcriptional level. Synthesis and incorporation into chromatin of the two histone proteins has been suggested to be involved in the epigenetic regulation of gene expression, both in normal brain development a…

Settore BIO/10 - BiochimicaHistone H1.0 Histone H3.3 Post-transcriptional regulation RNA-binding proteins (RBPs) rat brain maturationSettore BIO/06 - Anatomia Comparata E Citologia
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Expression and intracellular localization of H1° mRNA-containing complexes in developing rat brain and astrocytes

2015

INTRODUCTION: Post-transcriptional regulation of gene expression relies on RNA-binding proteins (RBPs), which regulate intracellular transport, stability, and translation of mRNAs [1]. We previously identified a set of proteins which interact with mRNAs encoding H1° and H3.3 histones [2-5]. All these proteins are probably part of a ribonucleoprotein particle [6]. Here we report more details on the expression and intracellular localization of some of these RBPs, during rat brain development and in isolated rat astrocytes. METHODS: Affinity chromatography was performed as already described [6]. Preparation of total lysates and cellular sub-fractions was done as reported in [3]. Possible co-lo…

Settore BIO/10 - BiochimicaPost-transcriptional regulation RNA-binding proteins (RBPs) H1° and H3.3 histones variants CSD-C2.Settore BIO/06 - Anatomia Comparata E Citologia
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Extended characterization of hypoimmunogenic mesenchymal stem cells isolated from the subendocardial layer of human hearts from chronic heart failure…

2009

Purpose: Mesenchymal stem cells (MSC) are pluripotent cells which are known to reside in a number of adult organs. In human heart, various populations of stem/progenitor cells have been isolated by different groups. Several efforts still need to be made to better characterize resident or migrating MSC populations in terms of markers expression and immunogenic potential prior to their use for regenerative medicine applications in heart diseases. Methods: MSC were isolated from the sub-endocardial layer of left ventricle sections of hearts explanted from patients affected by chronic heart failure (CHF). The expression of several markers characteristic of the MSC lineage was assessed at both t…

Settore BIO/16 - Anatomia Umanamesenchymal stem cells chronic heart failure differentiation markers transcription factor immune regulation
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Collective Reasoning over Shared Concepts for the Linguistic Atlas of Sicily

2013

In this chapter, collective intelligence principles are applied in the context of the Linguistic Atlas of Sicily (ALS - Atlante Linguistico Siciliano), an interdisciplinary research focusing on the study of the Italian language as it is spoken in Sicily, and its correlation with the Sicilian dialect and other regional varieties spoken in Sicily. The project has been developed over the past two decades and includes a complex information system supporting linguistic research; recently it has grown to allow research scientists to cooperate in an integrated environment to produce significant scientific advances in the field of ethnologic and sociolinguistic research. An interoperable infrastruc…

Settore ING-INF/05 - Sistemi Di Elaborazione Delle InformazioniMarkup languagebusiness.industryData managementInteroperabilityCollective intelligenceContext (language use)computer.software_genreLinguisticsGeographyCommon knowledgeALSethnologic research sociolinguistic research Linguistic Atlas of Sicily collective reasoningshared concepts Data integration Social interaction Search engineEmergent knowledge Sociolinguistic Spoken Transcription Linguistic annotation Linguistic Phenomena Collective intelligence Information retrieval Audio analysis Wavesurfer Collective Knowledge System information exchange Knowledge Discovery in Database Linguistic framework Interviews Biographic information Meta-linguistic information Social characterization Statistic InformationDiatopiaFilologicheiBatis framework spectrogram analysisMarkup language Geographic information system Pronunciation Linguistic tools Data management SAX builder Results dissemination Geo-localized informationdata exploration geographically referenced visualizationbusinesscomputerInformation exchangeData integration
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Ancora sulle "esplosive palatali" nelle inchieste dell'Atlante Linguistico del Mediterraneo (ALM): i punti albanesi

2021

Tra i «Problemi redazionali» emersi in fase di controllo e organizzazione dei materiali giunti dalla campagna di rilevamenti dell’Atlante Linguistico del Mediterraneo (ALM), Berruto (1971-1973) rileva la proliferazione e l’eterogeneità di alcune scelte grafemiche operate da diversi raccoglitori che, impegnati in una trascrizione più o meno puntuale delle risposte ottenute, avevano interpretato e risolto variamente alcune delle indicazioni previste nel sistema di trascrizione fonetica che accompagnava il Questionario. Particolarmente complesso era emerso il quadro relativo alla trascrizione di alcune consonanti del settore “palatale”, per le quali i grafemi (e i foni a essi corrispondenti) p…

Settore L-FIL-LET/12 - Linguistica ItalianaAmong the «Editorial problems» that emerged during the control and organization of the materials coming from the survey campaign of the Atlante Linguistico del Mediterraneo (ALM) Berruto (1971-1973) notes the proliferation and heterogeneity of some graphemic choices made by different field researchers. As they were engaged in a more or less punctual transcription of the answers obtained they had interpreted and solved differently some of the directions provided in the phonetic transcription system that accompanied the Questionnaire. The picture relating to the transcription of some consonants of the palatal sector had emerged as particularly complex since the graphemes (and the corresponding phones) foreseen in the original system (ALM 1959) were insufficient. Not even the 1971 updates meant to improve the representation of some phonetic features emerged from the investigations in the operations of Response reviewing allowed to obtain a clear homogeneous picture regarding the «plosive palatal» consonants. With regard to the phonetic trait under scrutiny I have already had the opportunity to propose a path aiming to the achievement of possible operational solutions. This has taken place in view of the planned simplification / homologation and transcoding phase from the ALM original system to the IPA one - while proceeding from the examination of the Schedoni’s current state with regard to the surveys referring to central-southern Italy Corsica and Sicily (see Matranga 2019). The scope is now to investigate the same phonetic trait as proven in the materials about the surveys carried out by Lirak Dodbiba in the ALM’s three Albanian points.
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miRNA Signature and Dicer Requirement during Human Endometrial Stromal Decidualization In Vitro

2012

Decidualization is a morphological and biochemical transformation of endometrial stromal fibroblast into differentiated decidual cells, which is critical for embryo implantation and pregnancy establishment. The complex regulatory networks have been elucidated at both the transcriptome and the proteome levels, however very little is known about the post-transcriptional regulation of this process. miRNAs regulate multiple physiological pathways and their de-regulation is associated with human disorders including gynaecological conditions such as endometriosis and preeclampsia. In this study we profile the miRNAs expression throughout human endometrial stromal (hESCs) decidualization and analy…

Sexual ReproductionRibonuclease IIISmall interfering RNAAnatomy and PhysiologyCellular differentiationGene ExpressionBioinformaticsCell morphologyTranscriptomeEndocrinologyMolecular Cell BiologyMultidisciplinarybiologyStem CellsQDeciduaRObstetrics and GynecologyCell DifferentiationForkhead Transcription FactorsCell biologyFemale Genital Diseasesmedicine.anatomical_structureMedicineFemaleResearch ArticleAdultScienceMolecular GeneticsYoung AdultmicroRNAGeneticsDeciduamedicineReproductive EndocrinologyHumansGene RegulationBiologyEmbryonic Stem CellsHomeodomain ProteinsGene Expression ProfilingReproductive SystemComputational BiologyDecidualizationFibroblastsFemale SubfertilityInsulin-Like Growth Factor Binding Protein 1MicroRNAsHomeobox A10 ProteinsGene Expression Regulationbiology.proteinStromal CellsDevelopmental BiologyDicerPLoS ONE
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Oral and Vaginal Epithelial Cell Lines Bind and Transfer Cell-Free Infectious HIV-1 to Permissive Cells but Are Not Productively Infected

2014

The majority of HIV-1 infections worldwide are acquired via mucosal surfaces. However, unlike the vaginal mucosa, the issue of whether the oral mucosa can act as a portal of entry for HIV-1 infection remains controversial. To address potential differences with regard to the fate of HIV-1 after exposure to oral and vaginal epithelium, we utilized two epithelial cell lines representative of buccal (TR146) and pharyngeal (FaDu) sites of the oral cavity and compared them with a cell line derived from vaginal epithelium (A431) in order to determine (i) HIV-1 receptor gene and protein expression, (ii) whether HIV-1 genome integration into epithelial cells occurs, (iii) whether productive viral in…

Sexual transmissionTranscription GeneticVirus IntegrationScienceReceptors Cell SurfaceGenome ViralBiologyMicrobiologyCXCR4EpitheliumVirusCell LineFlow cytometryViral ProteinsImmunodeficiency VirusesmedicineHumansRNA MessengerOral mucosaMicrobial PathogensMultidisciplinarymedicine.diagnostic_testQMouth MucosaRBiology and Life SciencesHIVCorrectionEpithelial CellsVirologyMolecular biologyEpitheliumBiological Tissuemedicine.anatomical_structureGene Expression RegulationTranscytosisMedical MicrobiologyCell cultureViral PathogensDNA ViralVaginaHIV-1MedicineFemaleAnatomyResearch Article
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A novel cell wall protein specific to the mycelial form of Yarrowia lipolytica.

1996

A cDNA clone specifying a cell wall protein was isolated from a Yarrowia lipolytica cDNA library. The cDNA library was constructed in the expression vector lambda gt 11, with the RNA isolated from actively growing mycelial cells. The deduced amino acid sequence shows that the encoded protein contains an N-terminal hydrophobic signal peptide. We have designated this protein YWP1 for Yarrowia lipolytica cell Wall Protein. Northern hybridization identified YWP1 transcript only when Y. lipolytica was growing in the mycelial form. The encoded protein seems to be covalently bound to the glucan cell wall since it is not released from the cell walls by sodium dodecyl sulphate extraction, but it is …

Signal peptideDNA ComplementaryTranscription GeneticHydrolasesBlotting WesternGenetic VectorsMolecular Sequence DataRestriction MappingBioengineeringApplied Microbiology and BiotechnologyBiochemistryCell wallFungal ProteinsOpen Reading FramesTransformation GeneticCell WallComplementary DNAGene Expression Regulation FungalYeastsGeneticsEscherichia coliAmino Acid SequenceCloning MolecularFluorescent Antibody Technique IndirectPeptide sequenceAntibodies FungalGene LibraryExpression vectorbiologyBase SequencecDNA libraryRNASodium Dodecyl SulfateYarrowiaRNA Fungalbiology.organism_classificationBlotting NorthernBlotting SouthernBiochemistrySaccharomycetalesElectrophoresis Polyacrylamide GelBiotechnologyYeast (Chichester, England)
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