Search results for "Transcriptome Analysis"

showing 3 items of 13 documents

Cellular effects of bacterial N-3-Oxo-dodecanoyl-L-Homoserine lactone on the sponge Suberites domuncula (Olivi, 1792): insights into an intimate inte…

2014

International audience; Sponges and bacteria have lived together in complex consortia for 700 million years. As filter feeders, sponges prey on bacteria. Nevertheless, some bacteria are associated with sponges in symbiotic relationships. To enable this association, sponges and bacteria are likely to have developed molecular communication systems. These may include molecules such as N-acyl-L-homoserine lactones, produced by Gram-negative bacteria also within sponges. In this study, we examined the role of N-3-oxododecanoyl-L-homoserine lactone (3-oxo-C12-HSL) on the expression of immune and apoptotic genes of the host sponge Suberites domuncula. This molecule seemed to inhibit the sponge inn…

ProteomicsApoptosisPathogenesisPathology and Laboratory MedicineBiochemistrycaspase 74-Butyrolactonecaspase 3lcsh:ScienceCytoskeletoncaspase like 7 gene0303 health sciencesToll-like receptorMarine Ecologytoll like receptorGenomicsproto oncogeneEndocytosisCell biologySuberites domunculaCellular Structures and Organellesalpha actininCell signalingtoll like receptor associated factor 6Gram negative bacteriumparacrine signalingMicrobiology03 medical and health sciencesGeneticsRNA Messengerhost pathogen interactionprotein expressiontwo dimensional electrophoresisBacteria030306 microbiologyEcology and Environmental Scienceslcsh:RBiology and Life SciencesComputational BiologyImmunity Innatecarrier proteinSpongebacterial membranelcsh:Qimmunological toleranceSuberitesProtein AbundanceSuberitessuberites domuncula[SDV]Life Sciences [q-bio]lcsh:MedicineMolecular Cell BiologyMedicine and Health Sciencesinnate immunityperforinMultidisciplinaryEcologybiologymessenger RNAarticlecell communicationAnimal Modelsmatrix assisted laser desorption ionization time of flight mass spectrometryunclassified drugPoriferaHost-Pathogen InteractionscytotoxicityactinTranscriptome Analysishormone actionResearch ArticleSymbiotic bacteriaprotein bcl 2Marine BiologycofilinResearch and Analysis Methodsn (3 oxododecanoyl)homoserine lactoneMicrobial EcologycogninModel OrganismsHomoserineAnimalscontrolled study14. Life underwatergeneSymbiosiscell viabilityadenosine triphosphatase030304 developmental biologynonhumanChemical EcologyMembrane ProteinsCell Biologytumor necrosis factor receptor associated factor 6Genome Analysisbiology.organism_classificationalpha tubulinGene Expression RegulationMembrane proteingene expressioncaspase like 3 geneGenome Expression AnalysisBacteriaPLoS ONE
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Large-scale analysis of SARS-CoV-2 spike-glycoprotein mutants demonstrates the need for continuous screening of virus isolates

2021

Due to the widespread of the COVID-19 pandemic, the SARS-CoV-2 genome is evolving in diverse human populations. Several studies already reported different strains and an increase in the mutation rate. Particularly, mutations in SARS-CoV-2 spike-glycoprotein are of great interest as it mediates infection in human and recently approved mRNA vaccines are designed to induce immune responses against it. We analyzed 1,036,030 SARS-CoV-2 genome assemblies and 30,806 NGS datasets from GISAID and European Nucleotide Archive (ENA) focusing on non-synonymous mutations in the spike protein. Only around 2.5% of the samples contained the wild-type spike protein with no variation from the reference. Among…

RNA virusesMutation rateCoronavirusesEpidemiologyMolecular biologyT-LymphocytesMutantGene Identification and Analysismedicine.disease_causeGenomeWhite Blood CellsDatabase and Informatics MethodsSequencing techniquesMutation RateAnimal CellsDNA sequencingPathology and laboratory medicineGeneticsMutationMultidisciplinaryT CellsMicrobial MutationQRHigh-Throughput Nucleotide SequencingGenomicsMedical microbiologyVirusesSpike Glycoprotein CoronavirusMedicineSARS CoV 2PathogensCellular TypesTranscriptome AnalysisSequence AnalysisResearch ArticleNext-Generation SequencingSARS coronavirusBioinformaticsImmune CellsScienceImmunologyProtein domainSequence alignmentGenomicsGenome ViralBiologyMicrobiologyAntibodiesDNA sequencingProtein DomainsGeneticsmedicineHumansMutation DetectionPandemicsMedicine and health sciencesBlood CellsBiology and life sciencesSARS-CoV-2OrganismsViral pathogensComputational BiologyCOVID-19Cell BiologyGenome AnalysisMicrobial pathogensResearch and analysis methodsMolecular biology techniquesMutationSequence AlignmentPLOS ONE
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INVESTIGATION OF BIOTIC STRESS RESPONSES IN FRUIT TREE CROPS USING META-ANALYTICAL TECHNIQUES.

2020

In recent years, RNA sequencing and analysis using Next Generation Sequencing (NGS) methods have enabled to understand the gene expression pertaining to plant biotic and abiotic stress conditions in both quantitative and qualitative manner. The large number of transcriptomic works published in plants requires more meta-analysis studies that would identify common and specific features in relation of the high number of objective studies performed at different developmental and environmental conditions. Meta-analysis of transcriptomic data will identify commonalities and differences between differentially regulated gene lists and will allow screen which genes are key players in gene-gene and p…

Settore AGR/03 - Arboricoltura Generale E Coltivazioni ArboreeBiotic Stress Transcriptome Analysis Next Generation Sequencing Bioinformatics
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