Search results for "Transfection"

showing 10 items of 581 documents

Tumor-specific T cell activation by recombinant immunoreceptors: CD3 zeta signaling and CD28 costimulation are simultaneously required for efficient …

2001

Abstract Recombinant immunoreceptors with specificity for the carcinoembryonic Ag (CEA) can redirect grafted T cells to a MHC/Ag-independent antitumor response. To analyze receptor-mediated cellular activation in the context of CD28 costimulation, we generated: 1) CEA+ colorectal tumor cells that express simultaneously B7-1 and B7-2, and 2) CEA-specific immunoreceptors that harbor intracellularly the signaling moities either of CD28 (BW431/26-scFv-Fc-CD28), CD3ζ (BW431/26-scFv-Fc-CD3ζ), or FcεRIγ (BW431/26-scFv-Fc-γ). By retroviral gene transfer, we grafted activated T cells from the peripheral blood with these immunoreceptors. T cells that express the FcεRIγ or CD3ζ signaling receptor lyse…

CD4-Positive T-LymphocytesCD3 ComplexT cellCD3T-LymphocytesImmunologyEpitopes T-Lymphocytechemical and pharmacologic phenomenaBiologyCD8-Positive T-LymphocytesMajor histocompatibility complexLymphocyte ActivationTransfectionEpitopeAntigenCD28 AntigensAntigens NeoplasmmedicineTumor Cells CulturedImmunology and AllergyHumansReceptors ImmunologicReceptorReceptors IgGCD28hemic and immune systemsMolecular biologyCoculture TechniquesRecombinant ProteinsCell biologyCarcinoembryonic Antigenmedicine.anatomical_structurebiology.proteinB7-1 AntigenInterleukin-2CD8Signal TransductionJournal of immunology (Baltimore, Md. : 1950)
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In vitro generation of CD4+CD25+ regulatory cells from murine naive T cells

2007

CD4+ CD25+ regulatory T cells (Tregs) are crucial for the maintenance of immunological tolerance. Recent data indicate that Tregs not only develop in the thymus during ontogeny but can also differentiate from naive T cells in the periphery. The following protocol describes a method by which Tregs are generated in vitro by stimulation of naive T cells in the presence of transforming growth factor beta (Ti-Tregs). In vitro-induced regulatory T cells express markers of conventional Treg such as CD25 and the genetic program committing transcription factor FoxP3. Functionally the in vitro-generated Ti-Tregs suppress T-cell activation and proliferation while in vivo these cells have been proven t…

CD4-Positive T-LymphocytesCD3 ComplexT-Lymphocytesmedicine.medical_treatmentchemical and pharmacologic phenomenaBiologyBioinformaticsT-Lymphocytes RegulatoryGeneral Biochemistry Genetics and Molecular BiologyMiceInterleukin 21CD28 AntigensmedicineAnimalsCytotoxic T cellIL-2 receptorInterleukin 3Mice Inbred BALB CInterleukin-2 Receptor alpha SubunitFOXP3hemic and immune systemsTransfectionImmunotherapyTransforming growth factor betaCell biologyCD4 Antigensbiology.proteinBiomarkersNature Protocols
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Targeting the activation-induced antigen CD137 can selectively deplete alloreactive T cells from antileukemic and antitumor donor T-cell lines.

2006

AbstractIn HLA-incompatible hematopoietic stem cell transplantation, alloreactive donor T cells recognizing recipient mismatch HLA cause severe graft-versus-host disease (GVHD). Strategies allowing the selective depletion of alloreactive T cells as well as the enhancement of graft-versus-malignancy immunity would be beneficial. We generated donor CD8 T-cell lines in vitro using allogeneic recipient cells mismatched at a single HLA class I allele or haplotype as stimulators. Recipient cells were obtained from acute myeloid leukemias, renal-cell carcinomas, and CD40L-induced B lymphoblasts. Resulting alloreactive T cells were activated by incubating day 21 T-cell cultures with HLA-mismatch tr…

CD4-Positive T-LymphocytesHerpesvirus 4 HumanIsoantigensT cellImmunologyCD40 LigandCytomegalovirusGraft vs Host DiseaseHuman leukocyte antigenBiologyCD8-Positive T-LymphocytesIn Vitro TechniquesLymphocyte ActivationTransfectionBiochemistryImmunotherapy AdoptiveLymphocyte DepletionTumor Necrosis Factor Receptor Superfamily Member 9AntigenHLA AntigensT-Lymphocyte SubsetsmedicineCytotoxic T cellHumansCarcinoma Renal CellCells CulturedSkinB-LymphocytesImmunomagnetic SeparationLymphoblastCD137Cell BiologyHematologyT lymphocyteFibroblastsCytotoxicity Tests ImmunologicKidney Neoplasmsmedicine.anatomical_structureLeukemia MyeloidHistocompatibilityImmunologyK562 CellsCD8Blood
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Human dendritic cells transfected with allergen-DNA stimulate specific immunoglobulin G4 but not specific immunoglobulin E production of autologous B…

2007

Atopic/allergic diseases are characterized by T helper 2 (Th2)-dominated immune responses resulting in immunoglobulin E (IgE) production. DNA-based immunotherapies have been shown to shift the immune response towards Th1 in animal models. In further studies we showed that human dendritic cells (DC) transfected with allergen-DNA are able to stimulate autologous CD4(+) T cells from atopic individuals to produce Th1 instead of Th2 cytokines and to activate interferon-gamma (IFN-gamma)-producing CD8(+) T cells. The aim of this study was to analyse whether DC transfected with allergen-DNA are also able to influence immunoglobulin production of B cells from atopic donors. For this purpose, human …

CD4-Positive T-LymphocytesHypersensitivity ImmediateAllergyImmunologyCD8-Positive T-Lymphocytesmedicine.disease_causeImmunoglobulin ELymphocyte ActivationTransfectionAllergenImmune systemmedicineImmunology and AllergyHumansCells CulturedCell ProliferationPlant ProteinsRhinitisB-LymphocytesCD40biologyTransfectionOriginal ArticlesDendritic CellsAllergensImmunoglobulin ETh1 Cellsmedicine.diseaseCoculture TechniquesImmunoglobulin GImmunologybiology.proteinCytokinesAntibodyCD8T-Lymphocytes Cytotoxic
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Modification of the human allergic immune response by allergen-DNA-transfected dendritic cells in vitro.

2004

Abstract Background Atopic-allergic diseases are characterized by T H 2-dominated immune responses, resulting in IgE production. DNA-based immunotherapies have been shown to shift the immune response toward a T H 1-type response in animal models. Objective The aim of the study was to analyze whether dendritic cells (DCs) transfected with allergen-DNA conjugates are able to stimulate human autologous CD4 + T cells, CD8 + T cells, or both from atopic individuals to produce T H 1 cytokines instead of T H 2 cytokines. Methods For this purpose, human mature DCs from atopic donors were transfected with an adenovirus encoding the allergen Phl p 1. Autologous CD4 + and CD8 + T cells were stimulated…

CD4-Positive T-LymphocytesHypersensitivity Immediatemedicine.medical_treatmentImmunologyGenetic Vectorschemical and pharmacologic phenomenaBiologyCD8-Positive T-LymphocytesLymphocyte ActivationTransfectionInterleukin 21Interferon-gammaImmune systemmedicineImmunology and AllergyCytotoxic T cellHumansIL-2 receptorAntigen-presenting cellCells CulturedPlant ProteinsAdenoviruses HumanDendritic cellDendritic CellsAllergensTh1 CellsMolecular biologyCytokineImmunologyCytokinesCD8The Journal of allergy and clinical immunology
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Regulation of Protein-DNA Interactions at the Interferon-gamma Gene Promoter by Corticosteroids: Implications for Inflammatory Bowel Diseases

1998

CD4-Positive T-LymphocytesRecombinant Fusion ProteinsProtein dnaInterferon-gamma biosynthesisTransfectionGeneral Biochemistry Genetics and Molecular BiologyInterferon-gammaHistory and Philosophy of ScienceAdrenal Cortex HormonesGenes ReporterT-Lymphocyte SubsetsmedicineHumansInterferon gammaPromoter Regions GeneticGenebusiness.industryGeneral NeuroscienceInflammatory Bowel DiseasesPromoterTransfectionInflammatory Bowel DiseasesTranscription Factor AP-1ImmunologyLeukocyte Common AntigensCancer researchLeukocyte Common Antigensbusinessmedicine.drugAnnals of the New York Academy of Sciences
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miR-155 inhibition sensitizes CD4+ Th cells for TREG mediated suppression.

2009

BackgroundIn humans and mice naturally occurring CD4(+)CD25(+) regulatory T cells (nTregs) are a thymus-derived subset of T cells, crucial for the maintenance of peripheral tolerance by controlling not only potentially autoreactive T cells but virtually all cells of the adaptive and innate immune system. Recent work using Dicer-deficient mice irrevocably demonstrated the importance of miRNAs for nTreg cell-mediated tolerance.Principal findingsDNA-Microarray analyses of human as well as murine conventional CD4(+) Th cells and nTregs revealed a strong up-regulation of mature miR-155 (microRNA-155) upon activation in both populations. Studying miR-155 expression in FoxP3-deficient scurfy mice …

CD4-Positive T-LymphocytesScienceImmunology/ImmunomodulationBiologyModels BiologicalT-Lymphocytes RegulatoryImmune tolerancemiR-155MiceDownregulation and upregulationImmune ToleranceAnimalsHumansIL-2 receptorOligonucleotide Array Sequence AnalysisMultidisciplinaryInnate immune systemGenetics and Genomics/Functional GenomicsQInterleukin-2 Receptor alpha SubunitRPeripheral toleranceFOXP3Forkhead Transcription FactorsTransfectionImmunity InnateCell biologyUp-RegulationKineticsMicroRNAsImmunologyImmunology/Immune ResponseMedicineGenetics and Genomics/Genetics of the Immune SystemResearch ArticlePLoS ONE
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Increased antigen presentation efficiency by coupling antigens to MHC class I trafficking signals.

2007

Abstract Genetic modification of vaccines by linking the Ag to lysosomal or endosomal targeting signals has been used to route Ags into MHC class II processing compartments for improvement of CD4+ T cell responses. We report in this study that combining an N-terminal leader peptide with an MHC class I trafficking signal (MITD) attached to the C terminus of the Ag strongly improves the presentation of MHC class I and class II epitopes in human and murine dendritic cells (DCs). Such chimeric fusion proteins display a maturation state-dependent subcellular distribution pattern in immature and mature DCs, mimicking the dynamic trafficking properties of MHC molecules. T cell response analysis in…

CD4-Positive T-LymphocytesT cellRecombinant Fusion ProteinsImmunologyAntigen presentationMolecular Sequence DataMice Inbred StrainsCD8-Positive T-LymphocytesProtein Sorting SignalsMajor histocompatibility complexTransfectionViral Matrix ProteinsEpitopesMiceAntigens NeoplasmMHC class ImedicineImmunology and AllergyAnimalsHumansAmino Acid SequenceAntigensMHC class IIAntigen PresentationbiologyAntigen processingHistocompatibility Antigens Class IVaccinationMembrane ProteinsDendritic CellsMHC restrictionPhosphoproteinsCell biologyProtein Transportmedicine.anatomical_structurebiology.proteinCD8Journal of immunology (Baltimore, Md. : 1950)
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Regulation of Protein-DNA Interactions at the Interferon-gamma Gene Promoter by Corticosteroids

1998

CD4-Positive T-LymphocytesTranscription GeneticRecombinant Fusion ProteinsProtein dnaBiologyLymphocyte ActivationTransfectionDexamethasoneGeneral Biochemistry Genetics and Molecular BiologyInterferon-gammaHistory and Philosophy of ScienceAdrenal Cortex HormonesAntigens CDGenes ReportermedicineHumansInterferon gammaInterleukin 29Promoter Regions GeneticCells CulturedGeneral NeurosciencePromoterTATA BoxMolecular biologyTranscription Factor AP-1Cancer researchLeukocyte Common AntigensTetradecanoylphorbol Acetatemedicine.drugAnnals of the New York Academy of Sciences
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The immunodominant CD8 T cell response to the human cytomegalovirus tegument phosphoprotein pp65(495-503) epitope critically depends on CD4 T cell he…

2011

Abstract Immunodominance hierarchies operating in immune responses to viral Ags limit the diversity of the elicited CD8 T cell responses. We evaluated in I-Ab+/A2-HHD-II and HLA-DR1+/A2-DR1 mice the HLA-A*0201–restricted, multispecific CD8 T cell responses to the human CMV tegument phosphoprotein pp65 (pp65) Ag. Vaccination of mice with pp65-encoding DNA elicited high IFN-γ+ CD8 T cell frequencies to the pp65495–503/(e6) epitope and low responses to the pp65320–328/(e3) and pp65522–530/(e8) epitopes. Abrogation of the e6-specific immunity efficiently enhanced e3- and e8-specific T cell responses by a pp65Δ501–503 DNA vaccine. The immunodominant e6-specific (but not the e3- and e8-specific) …

CD4-Positive T-LymphocytesvirusesT cellImmunologyEpitopes T-LymphocyteMice TransgenicImmunodominanceBiologyCD8-Positive T-LymphocytesLymphocyte ActivationTransfectionEpitopeDNA vaccinationViral Matrix ProteinsMiceImmune systemHLA-A2 AntigenmedicineImmunology and AllergyCytotoxic T cellAnimalsHumansAntigen-presenting cellHLA-A AntigensImmunodominant EpitopesVaccinationvirus diseasesPhosphoproteinsVirologyMolecular biologymedicine.anatomical_structureHEK293 CellsPhosphoproteinJournal of immunology (Baltimore, Md. : 1950)
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