Search results for "Typing"
showing 10 items of 1051 documents
A new tool for assessment of biostimulants effects on grapevine
2018
National audience; Despite an increasing interest for the use of biostimulants (BS) in agriculture, methods allowing a precise description of their effects on plants remain rather limited. In the IRIS+ FUI project, two major and highly different worldwide crops, wheat (annual, monocotyledon) and grapevine (perennial, broadleaf) were chosen to deepen our knowledge of such compounds and explore their potential additional interest. One objective was to develop a tool adapted to the screening and study of the impact of a series of BS on the development and physiology of these crops in controlled conditions. We managed to develop such a tool adapted to grapevine herbaceous cuttings. It allows a …
Phenotyping: Case study of legumes plants
2012
International audience; The activities of the GEAPSI group, belonging to the large research unit Agroecology, is devoted to the genetic improvement of legumes important for European agriculture, and focuses on the cultivated species pea (Pisum sativum) and the field bean (Vicia faba). These studies are supported by basic research on a model legume, Medicago truncatula, used for taking advantage of the extensive genomics information available. Some examples will first illustrate how the combination of analytical, non-destructive phenotypical measurements and modelling can improve our understanding of plant functioning, taking as case studies legumes plants. The presentation will then describ…
Mitochondrial genotyping of isolates of the arbuscular mycorrhizal fungus "Glomus intraradices" from the isolate collection of the International Bank…
2011
International audience; The culture collection of the International Bank of Glomeromycota (BEG) provides an important service to scientific community and industry by acting as a repository for well-defined AMF isolates which are made available to the public. The most widely-used species has been referred to as "Glomus intraradices", and was recently renamed Rhizophagus irregularis. This species has emerged as a model organism for experimental research and is the subject of the first AMF genome to be sequenced. The only genetic marker that can currently be used to distinguish strains of R. irregularis in field settings is the mitochondrial large subunit (mtLSU). In addition, genetically dist…
Rhizodeposition as a functional trait in legumes. Study of trade-off for plant productivity and resilience
2019
Rhizodeposition is the release of organic carbon (C) to the soil that connects the biotic and abiotic components of the C cycle. It can promote C storage to soil but also mediates plant-microbe interactions (Jones et al., 2009). These interactions are complexes as rhizodeposition will influence the composition and functioning of microbial populations which in return are able to increase the availability of nutrients in soil and provide protection against pathogens (Sasse et al., 2018). Despite their importance for current agriculture challenges, plant-soil microbes interactions remain poorly understood due to the methodological challenge they represent and the complexity of actors and proce…
In the context of sustainable agriculture how, with plant high throughput phenotyping, can we address the various challenges?
2018
International audience; In a context of climate change and soil and water resource degradation, it becomes increasingly important to reduce the need for high nutrient, water, or pesticides inputs, leading to more sustainable agricultural practices. In this context, our aim is to select the best performing crops in various deleterious abiotic environments, having both a higher yield and a better quality, a better environmental « efficiency ». Because plants interact with numerous and diverse microorganisms, especially in the soil volume surrounding roots, called rhizosphere, plant-microorganism relationships in the rhizosphere is also of great agronomical and ecological importance. To addres…
Does the memory of a first water deficit enable a more efficient response to a subsequent water deficit?
2021
National audience; In the current context of climate change, periods of water deficit occur more frequentlyalong the crop cycle, leading to high yield losses. To limit the negative impact of recurrentwater deficits, plants can adapt, via the mobilization of “stress memory”, allowing them torespond to a subsequent stress in a faster and/or more intensive manner. After a first stressevent, plants can keep an imprint of this stress via the induction of epigenetic (e.g. memorygene regulation), physiological (e.g. stomatal closure) and molecular (e.g. compoundaccumulation) changes. When maintained between two stress periods, these changes mayprepare plants for a subsequent water deficit.This wor…
A genetic and molecular approach to identify transcription factors controlling maize root adaptive response to water deficit
2022
Water stress is recognized as the most severe abiotic stress for agricultural productivity. Root traits play a key role in tolerance to water stress but have largely been neglected in selection schemes. In order to identify the maize genetic bases of the root adaptive responses to water deficit (WD), we used a MAGIC mapping population of 400 lines based on the intercrossing of 16 genotypes. The fine phenotyping of the different genotypes was performed under contrasting water supply on the French root phenotyping platform (4PMI). On the 16 founder genotypes, in addition of phenotyping, we sampled different root tissues daily over 7 days after irrigation arrest and performed RNAseq. On the ba…
Identification and diagnosis of patients with familial chylomicronaemia syndrome (FCS): Expert panel recommendations and proposal of an "FCS score".
2018
Familial chylomicronaemia syndrome (FCS) is a rare, inherited disorder characterised by impaired clearance of triglyceride (TG)-rich lipoproteins from plasma, leading to severe hypertriglyceridaemia (HTG) and a markedly increased risk of acute pancreatitis. It is due to the lack of lipoprotein lipase (LPL) function, resulting from recessive loss of function mutations in the genes coding LPL or its modulators. A large overlap in the phenotype between FCS and multifactorial chylomicronaemia syndrome (MCS) contributes to the inconsistency in how patients are diagnosed and managed worldwide, whereas the incidence of acute hypertriglyceridaemic pancreatitis is more frequent in FCS. A panel of Eu…
Failure to differentiate Cryptosporidium parvum from C. meleagridis based on PCR amplification of eight DNA sequences.
1998
ABSTRACT In order to determine the specificities of PCR-based assays used for detecting Cryptosporidium parvum DNA, eight pairs of previously described PCR primers targeting six distinct regions of the Cryptosporidium genome were evaluated for the detection of C. parvum , the agent of human cryptosporidiosis, and C. muris , C. baileyi , and C. meleagridis , three Cryptosporidium species that infect birds or mammals but are not considered to be human pathogens. The four Cryptosporidium species were divided into two groups: C. parvum and C. meleagridis , which gave the same-sized fragments with all the reactions, and C. muris and C. baileyi , which gave positive results with primer pairs targ…
An improved high yield method to obtain microsatellite genotypes from red deer antlers up to 200 years old.
2013
Analysis of DNA from older samples, such as museum specimen, is a promising approach to studying genetics of populations and ecological processes across several generations. Here, we present a method for extracting high quality nuclear DNA for microsatellite analysis from antlers of red deer (Cervus elaphus). The genotyping of individuals was based on nine microsatellite loci. Because the amount of DNA found in antlers was high, we could reduce the amount of sample and chemicals used and shorten the decalcification time in comparison to other methods. Using these methods, we obtained genotypes from antlers up to 200 years old.