Search results for "UNESCO::CIENCIAS DE LA VIDA::Bioquímica"
showing 10 items of 43 documents
Inhibición de complejos cdk/ciclina en modelos celulares de patología
2012
La proliferación celular descontrolada es el resultado de diversos mecanismos que conducen a un proceso patológico. Uno de ellos, es el desarrollo de aberraciones en cualquier punto de la maquinaria molecular que gobierna el ciclo celular y otro es la desregulación del receptor del factor de crecimiento epidérmico (EGFR). Los tratamientos tumorales convencionales han mejorado sin duda durante las últimas décadas, pero sigue siendo necesaria la búsqueda de nuevas estrategias terapéuticas. Las nuevas terapias están dirigiéndose hacia la inhibición simultanea de rutas proximales, puede favorecer la efectividad de los tratamientos antitumorales. En este trabajo de tesis presentamos los estudios…
Inhibition of liver trans-sulphuration pathway by propargylglycine mimics gene expression changes found in the mammary gland of weaned lactating rats…
2003
In the lactatingmammary gland, weaning produces mitochondrial cytochrome c release and nuclear DNA fragmentation, as determined by gel electrophoresis. This is followed by a significant decrease in lactation. Weaning for 2 h produces an early induction of the tumour suppressor/transcription factor p53, whereas the oncoprotein c-Jun and c-Jun N-terminal kinase are elevated after 24 h of weaning when compared with controls. The expression of p21cip1 and p27kip1, cyclin-dependent kinase inhibitors, was significantly higher in weaned rats when compared with control lactating rats. All the changes mentioned above also happen in the lactatingmammary gland when propargylglycine, an inhibitor of th…
Id2 leaves the chromatin of the E2F4–p130-controlled c-myc promoter
2006
The Id (inhibitor of DNA binding or inhibitor of differentiation) helix–loop–helix proteins are involved in the regulation of cell growth, differentiation and cancer. The fact that the molecular mechanisms of liver regeneration are not completely understood prompted us to study the fate of Id2 in proliferating liver. Id2 increases in liver regeneration after partial hepatectomy, following the early induction of its gene. Co-immunoprecipitation shows that Id2 forms a complex with E2F4, p130 and mSin3A in quiescent liver and all these components are present at the c-myc promoter as shown using ChIP (chromatin immunoprecipitation). Activation of c-myc during hepatocyte priming (G0–G1 transitio…
Estudio funcional de factores implicados en la transcripción y exportación de los RNAs en Saccharomyces cerevisiae
2014
La expresión génica en eucariotas ocurre a través del acoplamiento de diferentes procesos finamente regulados e interconectados en los que participan varios complejos multiprotéicos. Nuestro trabajo se centra en el estudio de las conexiones entre esos procesos que aseguran una correcta formación del ARN. La proteína Sus1 es un factor esencial para el correcto acoplamiento entre la transcripción y la exportación de los ARN mensajeros para su traducción en el citoplasma (1, 2). Sus1 es componente de 3 complejos diferentes: -TREX-2, asociado al complejo del poro nuclear y tiene funciones en el metabolismo del ARN mensajero, la exportación del mismo al citoplasma y en estabilidad genómica; -SAG…
Doble hélice, genes y cromosomas
2003
franco@uv.es
Bases estructurales de la regulación por nitrógeno mediada por la proteína señalizadora PII
2016
Tesis doctotal, 272 páginas, 72 figuras.
Discovery of novel pathways of microbial arginine biosynthesis
2012
The amino acid L-arginine is an essential component of all living organisms. Its importance resides in the variety of functions that arginine itself, along with some intermediary metabolites involved in its de novo synthesis in the cell. In many prokaryotes, fungi, and plants the de novo biosynthesis of arginine proceeds from glutamate in eight enzymatic steps (Figure 2). The first committed step of this pathway is the N-acetylation of glutamate. Acetylation of the early precursors of arginine distinguishes them from the analogous intermediates in the biosynthesis of proline. Although each and every step of the pathway is essential for its completion, transcarbamylation of ornithine to prod…
Mutations in the Bacillus thuringiensis Cry1Ca toxin demonstrate the role of domains II and III in specificity towards Spodoptera exigua larvae
2004
Several mutants of the Bacillus thuringiensis Cry1Ca toxin affected with regard to specific activity towards Spodoptera exigua were studied. Alanine was used to replace single residues in loops 2 and 3 of domain II (mutant pPB19) and to replace residues 541– 544 in domain III (mutant pPB20). Additionally, a Cry1Ca mutant combining all mutations was constructed (mutant pPB21). Toxicity assays showed a marked decrease in toxicity against S. exigua for all mutants, while they retained their activity against Manduca sexta, confirming the importance of these residues in determining insect specificity. Parameters for binding to the specific receptors in BBMV (brush border membrane vesicles) of S.…
Quadern de pràctiques de Mètodes en Biologia Molecular i Enginyeria Genètica
2011
El document inclou la descripció d'alguns experiments de laboratori sobre Enginyeria Genètica que són aplicables per a estudiants de graus de Bioquímica i Ciències Biomèdiques i de Biotecnologia El document forma part dels materials docents programats mitjançant l'ajut del Servei de Política Lingüística de la Universitat de València.
Conformation and concerted dynamics of the integrin-binding site and the C-terminal region of echistatin revealed by homonuclear NMR
2005
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