Search results for "Uber"

The role of traditional Erica arborea L. management practices in maintaining northeastern Sicily’s cultural landscape

2007

The tree heath (Erica arborea L.), a small evergreen tree found in the Mediterranean region and parts of Eastern and Central Africa, produces lignotubers that are highly valued for making smoking pipes. The Peloritani Mountains of northeastern Sicily are historically one of the most important areas in Italy for the production of these high quality lignotubers, known as briar root wood. In the past, tree heath shrublands were also an important source of charcoal. Erica arborea utilisation has declined dramatically during the past century, mostly due to the decreasing demand for smoking pipes, as well as the increasing frequency of wildfires and large-scale afforestation activities in this re…

José Vidal-Beneyto (1927-2010). In memoriam

2010

Polarity factor 'Frizzled' in the demosponge Suberites domuncula: identification, expression and localization of the receptor in the epithelium/pinac…

2003

Until recently, it was assumed that polarity and axis formation have evolved only in metazoan phyla higher than Cnidaria. One key molecule involved in the signal transduction causing tissue polarity is Frizzled, a seven-transmembrane receptor that is activated by the Wnt family of secreted proteins. We report the isolation and characterization of a Frizzled gene from the demosponge Suberites domuncula (Sd-Fz). The deduced polypeptide comprises all characteristic domains known from Frizzled receptors of higher metazoans. In situ hybridization studies show that Sd-Fz is expressed in cells close to the surface of the sponges and in the pinacocytes of some canals. Northern blot analysis demonst…

Isolation and characterization of Wnt pathway-related genes from Porifera.

2006

The Wnt signal acts by binding to Frizzled receptors, with the subsequent activation of two different signal transduction cascades, the canonical and the non-canonical Wnt pathways, involved in cell growth, differentiation, migration and fate. The canonical pathway functions through the translocation of beta-catenin to the nucleus and the activation of TCF/LEF transcription factors; it plays an important role in developmental patterning and cell fate decisions during embryogenesis. The non-canonical Wnt pathway is responsible for the planar cell polarity process in invertebrates, and for the convergent-extension movements during vertebrate gastrulation. The final effect of the non-canonical…

Isolation and Characterization of a New Less-Toxic Derivative of the Fusarium Mycotoxin Diacetoxyscirpenol after Thermal Treatment

2011

Trichothecenes are an important class of mycotoxins that act as potent protein synthesis inhibitors in eukaryotic organisms. The compound 4,15-diacetoxyscirpenol is highly toxic for plants and animals. Potatoes are especially prone to be contaminated with 4,15-diacetoxyscirpenol after infection with Fusarium sambucinum. In the current study, the reduction of 4,15-diacetoxyscirpenol during thermal treatment in aqueous solution was monitored. A new derivative was detected and named DAS-M1. After isolation, DAS-M1 was characterized with LC-HR-MS and LC-MS/MS and structurally elucidated with (1)H, (13)C, and 2D NMR. Potatoes were inoculated with F. sambucinum, and the infected potatoes were coo…

Ciclohexadespipeptide beauvericin degradation by different strains of Saccharomyces cerevisiae

2013

Abstract The interaction between the mycotoxin beauvericin (BEA) and 9 yeast strains of Saccharomyces cerevisiae named LO9, YE-2, YE5, YE-6, YE-4, A34, A17, A42 and A08 was studied. The biological degradations were carried out under aerobic conditions in the liquid medium of Potato Dextrose Broth (PDB) at 25 °C for 48 h and in a food/feed system composed of corn flour at 37 °C for 3 days, respectively. BEA present in fermented medium and corn flour was determined using liquid chromatography coupled to the mass spectrometry detector in tandem (LC–MS/MS) and the BEA degradation products produced during the fermentations were determined using the technique of the liquid chromatography coupled …

Atrophic dermatofibrosarcoma protuberans with the fusion gene COL1A1-PDGFB

2008

GDPR piekļuves tiesību sistēma UBER platformai

2019

Vispārējā datu aizsardzības regula (turpmāk tekstā – GDRP) ir regula, kuras mērķis ir nodrošināt un veicināt personas datu privātumu. Regula stājās spēkā 2018. gada 25. maijā. Visiem uzņēmumiem, kuri pārvalda Eiropas pilsoņu datus, bija jāievieš jaunu noteikumu kopumu. Viens no svarīgākajiem GDPR noteikumiem bija piekļuves tiesību noteikums. Tas dod datu subjektiem tiesības saņemt no kontroliera apstiprinājumu par to, vai tiek apstrādāti personas dati, kas attiecas uz subjektu, un, ja kontrolieris apstrādā subjekta personas datus, dod subjektam piekļuvi tiem. UBER ieņem vadošo pozīciju transporta nozarē. UBER dod iespēju lietotājam organizēt un plānot pārvadājumus. Lai saglabātu augstu pozī…

Expression pattern of the Brachyury and Tbx2 homologues from the sponge Suberites domuncula.

2005

Background information. T-box transcription factors are a large family of transcriptional regulators involved in many aspects of embryonic development. In a previous report, we described the isolation and genomic characterization of two T-box genes from the siliceous sponge Suberites domuncula: a Brachyury homologue, Sd-Bra, and a Tbx2 homologue, Sd-Tbx2. Elucidation of the genomic structure of Sd-Bra allowed us to demonstrate the existence of two different isoforms, resulting from alternative splicing. Moreover, we demonstrated that the shorter isoform exists in two different glycosylation states. Results. In the present study, we demonstrate a differential subcellular localization of the …

Analysis of the axial filament in spicules of the demosponge Geodia cydonium: different silicatein composition in microscleres (asters) and megascler…

2007

The skeleton of the siliceous sponges (Porifera: Hexactinellida and Demospongiae) is supported by spicules composed of bio-silica. In the axial canals of megascleres, harboring the axial filaments, three isoforms of the enzyme silicatein (-alpha, -beta and -gamma) have been identified until now, using the demosponges Tethya aurantium and Suberites domuncula. Here we describe the composition of the proteinaceous components of the axial filament from small spicules, the microscleres, in the demosponge Geodia cydonium that possesses megascleres and microscleres. The morphology of the different spicule types is described. Also in G. cydonium the synthesis of the spicules starts intracellularly …