Search results for "Ultra"
showing 10 items of 4451 documents
High-Performance TiO2 Nanoparticle/DOPA-Polymer Composites
2014
Many natural materials are complex composites whose mechanical properties are often outstanding considering the weak constituents from which they are assembled. Nacre, made of inorganic (CaCO 3 ) and organic constituents, is a textbook example because of its strength and toughness, which are related to its hierarchical structure and its well-defi ned organic–inorganic interface. Emulating the construction principles of nacre using simple inorganic materials and polymers is essential for understanding how chemical composition and structure determine biomaterial functions. A hard multilayered nanocomposite is assembled based on alternating layers of TiO 2 nanoparticles and a 3-hydroxytyramine…
Tomografia Sonica ed Ultrasonica per il supporto all’intervento di restauro della statua del togato di Petrara (I Sec. D.C.)
2008
Nel 2003, a Locri (RC), è stata ritrovata la statua-ritratto in marmo del c.d. “togato di Petrara” (I sec. d.C.), rappresentata in Fig. 1, come si presenta dopo il restauro. Si tratta, probabilmente di una statua onoraria di un magistrato del municipium locrese, alta circa m 2,16 ma spezzata in tre parti: piedi con basetta, testa e figura. Dal marzo al luglio 2008 sono state eseguite le operazioni di restauro del manufatto per ricomporre la statua e porla nuovamente in posizione eretta. Una notevole lesione, infatti, era visibile sul fianco sinistro e sul retro. La causa di tale lesione si individuava subito con la presenza di un grosso perno a sezione quadrata inserito già in antico per il…
Tomographic diagnostics of high-frequency electrodeless lamps in argon–mercury mixtures
2006
Tomographic reconstruction of spatial profiles of the mercury atom density in the excited state 7 3S1 in high-frequency electrodeless lamps (HFELs) has been performed. The measurements of the Hg 546.1 nm line emission intensity have been made for the HFELs in argon–mercury mixture depending on the operation regime with different cold spot temperatures in the range 31–98 °C. The maximum entropy-based algorithm was applied for the reconstruction of local emission coefficients from the integrated intensities. The emission coefficients are directly related to the local values of the mercury atom density in the excited state 7 3S1, the upper state of the 546.1 nm transition. Such an investigatio…
Ultrasound Effects after Post Space Preparation: An SEM Study
2006
The aim of this study was to evaluate the effect of ultrasonic treatment on occlusion of dentine tubules in root canal walls after post space preparation in endodontically treated teeth. Twenty-four premolars were instrumented and filled using warm vertical condensation; after post space preparation, they were divided into two groups. The control group was treated using the etching procedure. The experiment samples were treated with EDTA irrigation and ultrasound activation for 30 s before the etching procedure. The roots were divided and the canal walls were examined under SEM at 1000x magnification. The debris and open tubule marks were observed at 2, 6, and 10 mm levels using a three-ste…
Destabilized green fluorescent protein detects rapid removal of transcription blocks after genotoxic exposure
2007
High stabilities of reporter proteins and their messenger RNAs (mRNAs) interfere with the detection of rapid transient changes in gene expression, such as transcriptional blocks posed by genotoxic DNA lesions. We have modified a green fluorescent protein (GFP) gene within the episomal pMARS vector by addition of a fragment encoding for mouse ornithine decarboxylase (ODC) proline-glutamate-serine-threonine-rich (PEST) sequence in order to target the protein to the proteasomes and achieved an unprecedentedly fast GFP turnover in permanently transfected human cells. As early as 1 h after inhibition of protein synthesis by cycloheximide, the number of fluorescent cells decreased more than 5-fo…
New insights into the regulatory mechanisms of ppGpp and DksA on Escherichia coli RNA polymerase-promoter complex
2015
The stringent response modulators, guanosine tetraphosphate (ppGpp) and protein DksA, bind RNA polymerase (RNAP) and regulate gene expression to adapt bacteria to different environmental conditions. Here, we use Atomic Force Microscopy and in vitro transcription assays to study the effects of these modulators on the conformation and stability of the open promoter complex (RPo) formed at the rrnA P1, rrnB P1, its discriminator (dis) variant and lambda pR promoters. In the absence of modulators, RPo formed at these promoters show different extents of DNA wrapping which correlate with the position of UP elements. Addition of the modulators affects both DNA wrapping and RPo stability in a promo…
A method for genome-wide analysis of DNA helical tension by means of psoralen-DNA photobinding
2010
The helical tension of chromosomal DNA is one of the epigenetic landmarks most difficult to examine experimentally. The occurrence of DNA crosslinks mediated by psoralen photobinding (PB) stands as the only suitable probe for assessing this problem. PB is affected by chromatin structure when is done to saturation; but it is mainly determined by DNA helical tension when it is done to very low hit conditions. Hence, we developed a method for genome-wide analysis of DNA helical tension based on PB. We adjusted in vitro PB conditions that discern DNA helical tension and applied them to Saccharomyces cerevisiae cells. We selected the in vivo cross-linked DNA sequences and identified them on DNA …
Transcriptional activation of the small GTPase gene rhoB by genotoxic stress is regulated via a CCAAT element
2001
The gene encoding the Ras-related GTPase RhoB-specific is immediate-early inducible by genotoxic treatments. Regulation of transcriptional activation of rhoB is still unclear. Here we show that cells lacking either p53 or c-Fos are not different from wild-type cells with respect to the level of rhoB induction upon UV irradiation, indicating that these transcription factors are not crucial for stimulation of rhoB mRNA expression. Extracts from UV-irradiated and non-irradiated cells revealed similar DNA-binding activities to a 0.17 kb rhoB promoter fragment harboring the functional element(s) necessary for stimulation of rhoB by UV light. By means of immunoprecipitation we found that an ATF-2…
P1082COMPARISON OF THE REMOVAL OF PROTEIN-BOUND TOXINS AND LARGE MIDDLE MOLECULES WITH HIGH-FLUX PMMA AND MEDIUM CUT-OFF DIALYZERS
2020
Abstract Background and Aims Dialytic clearance of protein-bound toxins and large middle molecules is poor by diffusive treatment and limited by high-flux hemodialysis (HD) and on-line hemodiafiltration (OL-HDF), and only a few studies have examined how to improve their removal by other extracorporeal strategies. Since 2017, there is a new generation of polymethyl methacrylate (PMMA) membranes that suppress platelet adhesion on the membrane surface improving hemocompatibility and permeability, while also maintaining adsorption properties related to conventional PMMA membranes. Expanded HD (HDx) with medium cut-off (MCO) membranes has also recently been incorporated into clinical practice an…
[Non-invasive trans-cranial magnetic resonance imaging-guided focused ultrasounds surgery to treat neurologic disorders]
2016
Negli anni passati le applicazioni non invasive degli ultrasuoni focalizzati guidati da risonanza magnetica per il trattamento dei disordini neurologici sono state osta- colate da limiti tecnici oggi finalmente superati. In Italia, nel corso del 2015, sono stati effettuati i primi trattamenti utilizzando per la prima volta al mondo un comune scanner di risonanza magnetica da 1.5T. I risultati a oggi ottenuti con questa nuova metodica e le sue future possibili appli- cazioni sono molto promettenti e aprono la strada verso nuove opzioni terapeutiche, sicure ed efficaci, un tempo impensabili. In past years non-invasive clinical applications of magnetic resonance-guided focused ultrasound for t…