Search results for "Umana"

Umbilical cord revisited: From Wharton's jelly myofibroblasts to mesenchymal stem cells

2013

The umbilical cord (UC) is an essential part of the placenta, contributing to foetal development by ensuring the blood flow between mother and foetus. The UC is formed within the first weeks of gestation by the enclosure of the vessels (one vein and two arteries) into a bulk of mucous connective tissue, named Wharton's jelly (WJ) and lined by the umbilical epithelium. Since their first identification, cells populating WJ were described as unusual fibroblasts (or myofibroblasts). Recent literature data further highlighted the functional interconnection between UC and the resident cells. The UC represents a reservoir of progenitor populations which are collectively grouped into MSCs (mesenchy…

Retinal neurodegeneration and an innovative nanostructured approach in an iron overload in vivo model

2020

CARDIAC STEM CELLS AND BIOMATERIALS: INDUCTION OF MYOGENIC DIFFERENTIATION AND IMPLANTION OF BIOSYNTHETIC AND NATURAL MATRICES IN THE ADULT HEART

2014

Principal purpose of regenerative medicine for the cardiac tissue is to find the best way to inoculate stem cells in a specific myocardial area damage, improving their homing, integration and survival. To achieve this goal, the field of biomaterials is important to bypass this obstacles, modulating the environment for implanted cells and enhance CSC function in the heart. Biomaterials can mimic or include naturally occurring extracellular matrix and instruct stem cell function in different ways: promoting angiogenesis, enhancing stem cell engraftment and differentiation, and accelerating electromechanical integration of transplanted cells. The aim of this thesis was to assess whether the pr…

Isolation and characterization of circulating exosomes from blood in brain tumors

Exosomes (EXs) are crucial in mediating intercellular communication in physiological and pathological conditions. Accordingly, tumor-secreted EXs play an important role in regulating tumor malignancy. Tumor-derived exosomes contain proteins, mRNAs, and miRNAs, which can be delivered between different types of cells and even transferred to distant locations to influence the biological activities of tumors, such as proliferation, invasion and metastasis (1). Clinically, tumor-derived EXs may represent a target for therapeutic intervention and for the development of early diagnostic biomarkers (2). In our laboratory, we have focused on the isolation, morphological analyses, determination of par…

Hsp60 levels and mitochondrial biogenesis in skeletal muscle after endurance training

Anti-cancer effects of Pleurotus eryngii var. eryngii: an in vitro and in vivo models focusing on Heat Shock Proteins

2017

Heat shock proteins (Hsps) are highly expressed in a variety of cancer cells and are essential to their survival contributing to tumor cell propagation, metastasis, and protection against apoptosis]. Cancer is a leading cause of death worldwide. The current anti-cancer drugs available in market are not target specific and pose several side-effects and complications in clinical management of various forms of cancer, which highlights the urgent need for novel effective and less-toxic therapeutic approaches. Medicinal mushrooms have emerged as wonderful source of nutraceuticals, anti-oxidants, anticancer, prebiotic, anti-inflammatory, cardiovascular, anti-microbial, and anti-diabetic. The ongo…

Evaluation of neuropeptide Y expression during acetaldehyde withdrawal in rats. Focus on hippocampus and nucleus accumbens

2012

Stress-related neuropeptides are involved in setting up alcohol addiction. Ethanol is able to acutely induce CRH and ACTH release, while cronically a dampered response of the hypothalamus -pituitary- adrenal (HPA) axis has been observed. Also neuropeptide Y (NPY) has been shown to modulate ethanol consumption, and its central expression seems inversely correlated to ethanol intake. Recent in vivo and in vitro evidence have highlighted the key role of acetaldehyde (ACD), ethanol first metabolite, as a mediator of the central effects of ethanol, even as modulator of the neuropeptidergic transmission in the rat brain. The aim of this study was to investigate NPY immunoreactivity following a 4-…

Small airways in sedentary and endurance-trained dystrophic (mdx) mice.

2015

The effects of mild endurance exercise training on the small airways in mdx mice are unknown. We compared epithelial thickness and turnover, apoptosis, and stress marker expression in small airways of mdx mice and wild-type (WT) controls, at rest and during exercise training. Mdx and WT mice were randomly assigned to sedentary (mdx-S, n=17; WT-S, n=19) or trained (mdx- EX, n=14; WT-EX, n=16) groups. Low-intensity endurance training (running on a wheel) was done 5 d/wk for 6 wk at progressively increasing speed (rpm from 16 to 24) and time (15 min to 1 h). Lungs were processed for light microscopy and periodic acid Schiff (PAS) staining. Hsp60 and PCNA were quantified by immunohistochemistry…

Endurance training damages small airway epithelium in mice.

2005

Analisi di biomarcatori urinari e tissutali coinvolti nei meccanismi pro- o anti-fibrotici in pazienti con stenosi del giunto pielo-ureterale unilate…

2016

Stenosi del giunto pielo-ureterale, biomarcatori urinari, fibrosi, funzione renale, matrice extracellulare.