Search results for "Unithiol"

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Quantification of the Raf-C1 Interaction With Solid-Supported Bilayers

2002

By use of the quartz crystal microbalance technique, the interaction of the Raf-Ras binding domain (RafRBD) and the cysteine-rich domain Raf-C1 with lipids was quantified by using solid-supported bilayers immobilized on gold electrodes deposited on 5 MHz quartz plates. Solid-supported lipid bilayers were composed of an initial octanethiol monolayer chemisorbed on gold and a physisorbed phospholipid monolayer varying in its lipid composition as the outermost layer. The integrity of bilayer preparation was monitored by impedance spectroscopy. For binding experiments, a protein construct comprising the RafRBD and Raf-C1 linked to the maltose binding protein and a His tag, termed MBP-Raf-C1, wa…

Lipid BilayersPhospholipidBiosensing TechniquesMicroscopy Atomic ForceBiochemistrychemistry.chemical_compoundMonolayerLipid bilayerMolecular BiologyBilayerOrganic ChemistryUnithiolQuartz crystal microbalanceProtein Structure TertiaryProto-Oncogene Proteins c-rafDissociation constantCrystallographychemistryThermodynamicsMolecular Medicinelipids (amino acids peptides and proteins)AdsorptionGoldDimyristoylphosphatidylcholineProtein adsorptionBinding domainChemBioChem
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