Search results for "Unity"
showing 10 items of 3852 documents
A20 expression in dendritic cells protects mice from LPS-induced mortality
2014
DCs contribute to immune homeostasis under physiological conditions and regulate the immune activation during infection. The deubiquitinase A20 inhibits the activation of NF-κB-dependent immune reactions, and prevents the hyperactivation of DCs under steady-state conditions. However, the role of DC-specific A20 under pathological conditions is unknown. Here, we demonstrate that upon injection of low-dose LPS, mice with DC-specific A20 deletion (CD11c-Cre A20(fl/fl) ) died within 6 h, whereas A20(fl/fl) controls survived. LPS-induced mortality in CD11c-Cre A20(fl/fl) mice was characterized by increased serum levels of IL-2, IL-10, IL-12, IFN-γ, and TNF. Upon LPS stimulation, the activation o…
Evaluation of the Role of Candida albicans Agglutinin-Like Sequence (Als) Proteins in Human Oral Epithelial Cell Interactions
2012
The fungus C. albicans uses adhesins to interact with human epithelial surfaces in the processes of colonization and pathogenesis. The C. albicans ALS (agglutinin-like sequence) gene family encodes eight large cell-surface glycoproteins (Als1-Als7 and Als9) that have adhesive function. This study utilized C. albicans Δals mutant strains to investigate the role of the Als family in oral epithelial cell adhesion and damage, cytokine induction and activation of a MAPK-based (MKP1/c-Fos) signaling pathway that discriminates between yeast and hyphae. Of the eight Δals mutants tested, only the Δals3 strain showed significant reductions in oral epithelial cell adhesion and damage, and cytokine pro…
Global Functional Analyses of Cellular Responses to Pore-Forming Toxins
2011
Here we present the first global functional analysis of cellular responses to pore-forming toxins (PFTs). PFTs are uniquely important bacterial virulence factors, comprising the single largest class of bacterial protein toxins and being important for the pathogenesis in humans of many Gram positive and Gram negative bacteria. Their mode of action is deceptively simple, poking holes in the plasma membrane of cells. The scattered studies to date of PFT-host cell interactions indicate a handful of genes are involved in cellular defenses to PFTs. How many genes are involved in cellular defenses against PFTs and how cellular defenses are coordinated are unknown. To address these questions, we pe…
Candida albicans Yeast and Hyphae are Discriminated by MAPK Signaling in Vaginal Epithelial Cells
2011
We previously reported that a bi-phasic innate immune MAPK response, constituting activation of the mitogen-activated protein kinase (MAPK) phosphatase MKP1 and c-Fos transcription factor, discriminates between the yeast and hyphal forms of Candida albicans in oral epithelial cells (ECs). Since the vast majority of mucosal Candida infections are vaginal, we sought to determine whether a similar bi-phasic MAPK-based immune response was activated by C. albicans in vaginal ECs. Here, we demonstrate that vaginal ECs orchestrate an innate response to C. albicans via NF-κB and MAPK signaling pathways. However, unlike in oral ECs, the first MAPK response, defined by c-Jun transcription factor acti…
P38 MAP Kinase Signaling Is Required for the Conversion of CD4+CD25− T Cells into iTreg
2008
CD4+CD25+ regulatory T cells (Treg) are important mediators of immune tolerance. A subset of Treg can be generated in the periphery by TGF-beta dependent conversion of conventional CD4+CD25− T cells into induced Treg (iTreg). In chronic viral infection or malignancy, such induced iTreg, which limit the depletion of aberrant or infected cells, may be of pathogenic relevance. To identify potential targets for therapeutic intervention, we investigated the TGF-beta signaling in Treg. In contrast to conventional CD4+ T cells, Treg exhibited marked activation of the p38 MAP kinase pathway. Inhibition of p38 MAP kinase activity prevented the TGF-beta-dependent conversion of CD4+CD25− T cells into …
"Vi ønsker å bli sett, men ønsker ikke å bli sett rart på" Om materialitet og mennesker i bofellesskap og dagsentra innen psykisk helsetjeneste i kom…
2015
Masteroppgave psykisk helsearbeid- Universitetet i Agder, 2015 Studien retter søkelyset mot bofellesskap og dagsentra beregnet for mennesker med psykiske lidelser. Bakgrunnen for bygging av bofellesskap og dagsentra var den omfattende deinstitusjonaliseringen som fant sted i hele den vestlige verden etter andre verdenskrig. Hensikten med denne studien er å undersøke hvorvidt de materielle stedene støtter opp om intensjonene bak oppførelsen av bofellesskap og dagsentra; om stedene bidrar til gode levekår og et bedre liv for brukerne. Fokus er på materialitet, regler og rutiner med utgangspunkt i forholdet mellom menneskene og det bygde sted. Problemstillingen er følgende: Hvilken betydning k…
Mestring og verdighet En kvalitativ studie av meningsinnholdet i begrepene, slik de blir forstått av brukere, pårørende og ansatte innen psykisk hels…
2015
Masteroppgave psykisk helsearbeid- Universitetet i Agder, 2015
Møtet med den nye hverdagen Om mestring og tilpasning hos bosatte flyktninger
2015
Masteroppgave psykisk helsearbeid- Universitetet i Agder, 2015 The purpose of this investigation has been to explore the difficulties and challenges refugees living through in their attempts to adapt to Norwegian society. What causes stress in the new daily life, how does it affect them, and how do they handled it? This is a qualitative investigation, based on data from interviews done with three-year intervals, with four refugees, allowing to find out whether changes are caused by structural conditions, or due do to the individual's inner resources, or both? The theoretical framework is sourced from stress- and coping theory. A hermeneutic phenomenological method, and the findings are anal…
Opplevd psykisk helse og livskvalitet hos elever 3, 6 og 9 år etter at de gikk ut av tilrettelagt videregående skole Kvalitativ retrospektiv studie a…
2015
Masteroppgave psykisk helsearbeid- Universitetet i Agder, 2015 As a teacher at an adaptive organized secondary school for vocational training, I want to examine how former students perceive their mental health (MH) and quality of life (QoL) today. The purpose of the project is to see if the program has positively helped to strengthen their MH and their QoL, some years after leaving school. The research question is, "3, 6 and 9 years after the adapted secondary education - How does the former pupils experience their mental health and quality of life?" The study throws light on four sub-questions. 1. How is your experienced mental health and quality of life today? 2. What have contributed pos…
I gråsonen mellom skole og helse i undervisningen av voksne traumatiserte flyktninger : om skolens rolle i rehabiliteringen
2010
Masteroppgave i psykisk helsearbeid- Universitetet i Agder 2010 Title: In the grey zone between school and health in teaching traumatized adult refugees – the school’s role in rehabilitation. Background When providing classroom training for the integration of adult refugees, there will always be individuals that, due to psychosocial problems caused by earlier traumatic experiences, are unable to follow the normal progress of the rest of the class. This represents a huge challenge for the educational institution. Based on research on traumas in Denmark, a special education methodology has been developed, called rehabilitating pedagogics. The following study focuses on the grey zone between e…