Search results for "University"

showing 10 items of 1352 documents

Macrophage type modulates osteogenic differentiation of adipose tissue MSCs

2017

Since the reconstruction of large bone defects remains a challenge, knowledge about the biology of bone healing is desirable to develop novel strategies for improving the treatment of bone defects. In osteoimmunology, macrophages are the central component in the early stage of physiological response after bone injury and bone remodeling in the late stage. During this process, a switch of macrophage phenotype from pro-inflammatory (M1) to anti-inflammatory (M2) is observed. An appealing option for bone regeneration would be to exploit this regulatory role for the benefit of osteogenic differentiation of osteoprogenitor cells (e.g., mesenchymal stem cells; MSCs) and to eventually utilize this…

0301 basic medicineHistologyMacrophageOsteoimmunologyAdipose tissueBone healingCell CommunicationBiologyBone morphogenetic protein 2Bone remodelingCell LinePathology and Forensic MedicineMSC03 medical and health sciencesCalcification PhysiologicAll institutes and research themes of the Radboud University Medical CenterOsteogenesisOsteogenic differentiationHumansBone regenerationCell ProliferationBone InjuryMacrophagesMesenchymal stem cellCell PolarityCell DifferentiationMesenchymal Stem CellsRegular ArticleCell BiologyAlkaline PhosphataseCoculture TechniquesCell biology030104 developmental biologyReconstructive and regenerative medicine Radboud Institute for Molecular Life Sciences [Radboudumc 10]Adipose TissueGene Expression RegulationCell culture modelImmunologyCytokinesBiomarkersCell and Tissue Research
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Trends of extended-spectrum β-lactamase-producing Escherichia coli sequence type 131 and its H30 subclone in a French hospital over a 15-year period.

2016

International audience; Sequence type 131 (ST131) is a predominant lineage among extraintestinal pathogenic Escherichia coli. It plays a major role in the worldwide dissemination of E. coli producing extended-spectrum β-lactamases (ESBLs). Here we describe the long-term epidemiology of this clonal group in a French university hospital, where the incidence of ESBL-producing E. coli has increased from 0.018 case per 1000 patient-days in the year 2000 to 0.50 case per 1000 patient-days in 2014. The first of the 141 ST131 isolates was recovered in 2006, and the ST131 clonal group accounted for 18.1% of total ESBL-producing E. coli over the whole period (2000-2014). Subclonal typing showed that …

0301 basic medicineMESH : Escherichia coliMESH : Retrospective StudiesMESH : Multilocus Sequence TypingMESH: beta-LactamasesMESH : GenotypeMultidrug resistancemedicine.disease_causeHospitals UniversityMESH: Genotype[ SDV.MP ] Life Sciences [q-bio]/Microbiology and ParasitologyPharmacology (medical)MESH: IncidenceMESH: Genetic VariationEscherichia coli InfectionsComputingMilieux_MISCELLANEOUSCross InfectionMolecular EpidemiologyExtraintestinal Pathogenic Escherichia coliMESH: Escherichia coliIncidenceIncidence (epidemiology)MESH : beta-LactamasesGeneral MedicinePFGEMESH : IncidenceElectrophoresis Gel Pulsed-Field3. Good healthInfectious DiseasesMESH: Electrophoresis Gel Pulsed-FieldMESH: Multilocus Sequence Typing[SDV.MP]Life Sciences [q-bio]/Microbiology and ParasitologyMESH : Escherichia coli Infections[SDE]Environmental SciencesFranceMESH : Cross InfectionMicrobiology (medical)clone (Java method)Lineage (genetic)GenotypeMESH : Molecular Epidemiology030106 microbiologyBiologybeta-LactamasesMicrobiology03 medical and health sciencesExtended-spectrum β-lactamaseMESH : Genetic VariationEscherichia coliPulsed-field gel electrophoresismedicineHumansMESH: Molecular EpidemiologyTypingMESH : FranceEscherichia coliMESH : Hospitals UniversityRetrospective StudiesMESH : Electrophoresis Gel Pulsed-FieldMESH: Escherichia coli InfectionsMESH: Hospitals UniversityMESH: HumansMESH : HumansGenetic VariationMESH: Cross InfectionMESH: Retrospective Studiesbacterial infections and mycosesMultiple drug resistanceMESH: FranceESBLMultilocus Sequence Typing
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Expanding the Phenotype Associated with NAA10-Related N-Terminal Acetylation Deficiency

2016

International audience; N-terminal acetylation is a common protein modification in eukaryotes associated with numerous cellular processes. Inherited mutations in NAA10, encoding the catalytic subunit of the major N-terminal acetylation complex NatA have been associated with diverse, syndromic X-linked recessive disorders, whereas de novo missense mutations have been reported in one male and one female individual with severe intellectual disability but otherwise unspecific phenotypes. Thus, the full genetic and clinical spectrum of NAA10 deficiency is yet to be delineated. We identified three different novel and one known missense mutation in NAA10, de novo in 11 females, and due to maternal…

0301 basic medicineMaleModels MolecularMicrocephalyMutation MissenseBiologyGermlineKEY WORDS: NAA1003 medical and health sciencesGermline mutationGenes X-LinkedIntellectual disabilityGeneticsmedicineMissense mutationHumansGenetic Predisposition to DiseaseN-Terminal Acetyltransferase EGenetics (clinical)Genetic Association StudiesGerm-Line MutationN-Terminal Acetyltransferase AResearch ArticlesGeneticsX-linked[SDV.GEN]Life Sciences [q-bio]/GeneticsRegional Council of BurgundyMosaicismN-terminal acetylationAcetylationmedicine.diseasePhenotypePedigreeOgden SyndromeX‐linked030104 developmental biologyNAA10intellectual disabilityN‐terminal acetylationContract grant sponsors: Dijon University HospitalFemale[ SDV.GEN ] Life Sciences [q-bio]/GeneticsNAA15Research ArticleHuman Mutation
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Prospective cohort study of risk factors for extended-spectrum ß-lactamase-producing Escherichia coli urinary tract infections in elderly patients ad…

2017

Producción Científica

0301 basic medicineMalePediatricsmedicine.medical_specialtyMultivariate analysismedicine.drug_classUrinary system030106 microbiologyAntibioticsbeta-Lactamases03 medical and health sciences0302 clinical medicineRisk Factors32 Ciencias Médicaspolycyclic compoundsmedicinePrevalenceEscherichia coliHumans030212 general & internal medicineProspective StudiesRisk factorProspective cohort studyEscherichia coli InfectionsAgedHigh rateAged 80 and overInfeccionesbusiness.industryMedical recordGeneral Medicinebiochemical phenomena metabolism and nutritionbacterial infections and mycosesUniversity hospitalHospitalizationSpainUrinary Tract InfectionsbacteriaFemalebusinessBiomarkers
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Impact of the BioFire FilmArray gastrointestinal panel on patient care and infection control.

2020

Contains fulltext : 218876.pdf (Publisher’s version ) (Open Access) OBJECTIVES: Conventional routine PCR testing for gastrointestinal infections is generally based on pathogen related panels specifically requested by clinicians and can be erroneous and time consuming. The BioFire FilmArray gastrointestinal (GI) panel combines 22 pathogens into a single cartridge-based test on a random-access system, thereby reducing the turnaround time to less than 2 hours. We described the clinical impact of implementing the BioFire FilmArray on patients with gastroenteritis in our hospital. METHODS: Patients attending a Dutch tertiary care center (Radboud University Medical Center), from whom stool sample…

0301 basic medicineMaleTime Factorslnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4]Pathology and Laboratory MedicineTertiary carePolymerase Chain ReactionTertiary Care CentersFeces0302 clinical medicineClinical historyAntibioticsMedicine and Health SciencesMedicineInfection controlUniversity medicalGastrointestinal Infections030212 general & internal medicineChildNetherlandsAged 80 and overPotential impactMultidisciplinaryWomen's cancers Radboud Institute for Molecular Life Sciences [Radboudumc 17]AntimicrobialsQRDrugsGastrointestinal AnalysisMiddle AgedGastroenteritisBacterial PathogensBioassays and Physiological AnalysisMolecular Diagnostic TechniquesMedical MicrobiologyChild PreschoolViral PathogensVirusesMedicinePathogensResearch ArticleAdultmedicine.medical_specialtyIsolation (health care)AdolescentClostridium DifficileScience030106 microbiologyGastroenterology and HepatologyResearch and Analysis MethodsMicrobiologyPatient care03 medical and health sciencesYoung AdultDiagnostic MedicineInternal medicineMicrobial ControlHumansMicrobial PathogensAgedPharmacologyInfection ControlBacteriabusiness.industryGut BacteriaInfant NewbornOrganismsInfantBiology and Life SciencesPatient datalnfectious Diseases and Global Health Radboud Institute for Health Sciences [Radboudumc 4]Patient CarebusinessPloS one
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Delivery of oral nutrition supplement in hospital: Evaluation of professional practices in evaluation of nutritional status and representations of ON…

2020

Journal non répertorié dans le JCR.; International audience; BACKGROUND: Nutritional evaluation and detection of malnutrition are based on criteria recommended by French health authorities. In practice, doctors do not always ensure strict implementation of the recommendations. The aim of this study is to evaluate professional practices in France regarding nutritional follow-up on arrival, during and after the hospitalization of inpatients who have oral nutritional supplements (ONS) prescribed and to discuss how ONS are seen by medical staff and patients. METHODS: A prospective study including patients consecutively admitted to digestive surgery and endocrinology units of the Dijon universit…

0301 basic medicineMalerepresentationEndocrinology Diabetes and MetabolismAdministration Oral[SHS]Humanities and Social Sciences0302 clinical medicineWeight lossProspective StudiesProspective cohort studyComputingMilieux_MISCELLANEOUS2. Zero hungerAged 80 and overNutrition and DieteticsNutritional statusProfessional PracticeMiddle AgedUniversity hospital3. Good healthHospitalizationCaregiversoral nutritional supplementsFemaleFrance[SHS] Humanities and Social Sciencesmedicine.symptomAdultmedicine.medical_specialty[SHS.INFO]Humanities and Social Sciences/Library and information sciencesNutritional Status030209 endocrinology & metabolism[SHS.INFO] Humanities and Social Sciences/Library and information sciences03 medical and health sciencesYoung AdultWeight LossmedicineHumansIn patientAged030109 nutrition & dieteticsbusiness.industryMalnutritionAnthropometryLength of Staymedicine.diseaseMalnutritionNutrition AssessmentFamily medicineDietary Supplementsbusiness[SDV.AEN]Life Sciences [q-bio]/Food and NutritionBody mass indexFollow-Up Studies
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Real-time polymerase chain reaction detection of Lichtheimia species in bandages associated with cutaneous mucormycosis in burn patients

2018

Summary Background Cutaneous mucormycoses, mainly due to Lichtheimia (Absidia), have occurred on several occasions in the Burn Unit of the University Hospital of Lille, France. Aim To investigate the potential vector role of non-sterile bandages used to hold in place sterile gauze used for wound dressing. Methods Mycological analysis by conventional culture, Mucorales real-time polymerase chain reaction (qPCR), and Lichtheimia species-specific qPCR were performed on eight crepe and six elasticized bandages that were sampled on two independent occasions in March 2014 and July 2016. Characteristics of the seven Lichtheimia mucormycoses which occurred in burn patients between November 2013 and…

0301 basic medicineMicrobiology (medical)MucoralesAdultMalemedicine.medical_specialtyLichtheimia corymbifera030106 microbiologyBurnReal-Time Polymerase Chain Reaction[ SDV.EE.SANT ] Life Sciences [q-bio]/Ecology environment/HealthHospitals UniversityCutaneous mucormycosis03 medical and health sciencesBandageMucorales qPCR0302 clinical medicineAbsidiaMedicineDermatomycosesHumansMucormycosis030212 general & internal medicineAged[SDV.EE.SANT]Life Sciences [q-bio]/Ecology environment/HealthCutaneous mucormycosisbiologybusiness.industryMucormycosisGeneral MedicineMiddle Agedbiology.organism_classificationmedicine.diseaseUniversity hospitalDermatologyBandages3. Good healthLichtheimia speciesInfectious DiseasesReal-time polymerase chain reactionMolecular Diagnostic TechniquesMucoralesFemaleFrancebusinessBurnsBandage
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A Snapshot on MRSA Epidemiology in a Neonatal Intensive Care Unit Network, Palermo, Italy

2016

Objectives. We performed a one-year prospective surveillance study on MRSA colonization within the five NICUs of the metropolitan area of Palermo, Italy. The purpose of the study was to assess epidemiology of MRSA in NICU from a network perspective. Methods. Transfer of patients between NICUs during 2014 was traced based on the annual hospital discharge records. In the period February 2014 – January 2015, in the NICU B, at the University teaching hospital, nasal swabs from all infants were collected weekly, whereas in the other four NICUs (A, C, D, E) at four week-intervals of time. MRSA isolates were submitted to antibiotic susceptibility testing, SCCmec typing, PCR to detect lukS-PV and l…

0301 basic medicineMicrobiology (medical)medicine.medical_specialtyNeonatal intensive care unithealth care facilities manpower and services030106 microbiologylcsh:QR1-502MRSAMicrobiologylcsh:Microbiology03 medical and health sciencesInternal medicineEpidemiologyMedicineInfection controlnetwork approachOriginal ResearchNICuActive surveillance; Molecular typing; MRSA; Network approach; NICu; Microbiology; Microbiology (medical)business.industryMRSA colonizationactive surveillancemolecular typingSequence typesVariable number tandem repeat030104 developmental biologyMultilocus sequence typingUniversity teachingbusinessFrontiers in Microbiology
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Influenza vaccination of healthcare workers in Italy: could mandatory vaccination be a solution to protect patients?

2019

Several studies have reported that influenza infections in healthcare workers (HCWs) can lead to nosocomial outbreaks. HCWs can potentially be infected with influenza every year, and may continue to work, encouraging the spread of the virus. Different strategies, such as informative interventions on influenza and influenza vaccination, ‘onsite’ vaccination weeks, communicative strategies through dedicated web and social media pages, and mandatory informed dissent form, were organized for HCWs working at the University Hospital of Palermo, during previous influenza seasons. However, the increased vaccination rates observed among HCWs still remain far from the 75% recommended by Public Healt…

0301 basic medicineMicrobiology (medical)medicine.medical_specialtyVaccination CoverageAttitude of Health Personnelhealth care facilities manpower and servicesHealth Personneleducation030106 microbiologyPsychological interventionMandatory ProgramsSettore MED/42 - Igiene Generale E ApplicataMicrobiologyInfectious Disease Transmission Professional-to-PatientHospitals University03 medical and health sciencesHealth careInfluenza HumanmedicineHumansNosocomial outbreakbusiness.industryPublic healthVaccinationvirus diseasesUniversity hospitalMandatory vaccinationcommunication strategies healthcare workers influenza disease perception influenza vaccination mandatory vaccinationVaccination030104 developmental biologyItalyInfluenza VaccinesFamily medicinebusinessFuture microbiology
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C2orf69 mutations disrupt mitochondrial function and cause a multisystem human disorder with recurring autoinflammation

2021

BACKGROUND. Deciphering the function of the many genes previously classified as uncharacterized open reading frame (ORF) would complete our understanding of a cell’s function and its pathophysiology. METHODS. Whole-exome sequencing, yeast 2-hybrid and transcriptome analyses, and molecular characterization were performed in this study to uncover the function of the C2orf69 gene. RESULTS. We identified loss-of-function mutations in the uncharacterized C2orf69 gene in 8 individuals with brain abnormalities involving hypomyelination and microcephaly, liver dysfunction, and recurrent autoinflammation. C2orf69 contains an N-terminal signal peptide that is required and sufficient for mitochondrial…

0301 basic medicineMicrocephalyRespiratory chainBiologyMitochondrionCell LineMitochondrial ProteinsTranscriptomeMiceOpen Reading Frames03 medical and health sciencesAll institutes and research themes of the Radboud University Medical Center0302 clinical medicineLoss of Function MutationGlycogen branching enzymemedicineAnimalsHumansGeneMice KnockoutGeneticsMetabolic Disorders Radboud Institute for Molecular Life Sciences [Radboudumc 6]Glycogen Debranching Enzyme SystemGeneral Medicinemedicine.diseaseMitochondriaOpen reading frameRenal disorders Radboud Institute for Molecular Life Sciences [Radboudumc 11]030104 developmental biology030220 oncology & carcinogenesisMicrocephalybiology.proteinClinical MedicineSignal transductionGlycogenJournal of Clinical Investigation
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