Search results for "Uses"
showing 10 items of 1614 documents
Nuclear entry and egress of parvoviruses.
2022
Parvoviruses are small non-enveloped single-stranded DNA viruses, which depend on host cell nuclear transcriptional and replication machinery. After endosomal exposure of nuclear localization sequence and a phospholipase A2 domain on the capsid surface, and escape into the cytosol, parvovirus capsids enter the nucleus. Due to the small capsid diameter of 18–26 nm, intact capsids can potentially pass into the nucleus through nuclear pore complexes (NPCs). This might be facilitated by active nuclear import, but capsids may also follow an alternative entry pathway that includes activation of mitotic factors and local transient disruption of the nuclear envelope. The nuclear entry is followed b…
Viroporins, Examples of the Two-Stage Membrane Protein Folding Model
2015
Viroporins are small, α-helical, hydrophobic virus encoded proteins, engineered to form homo-oligomeric hydrophilic pores in the host membrane. Viroporins participate in multiple steps of the viral life cycle, from entry to budding. As any other membrane protein, viroporins have to find the way to bury their hydrophobic regions into the lipid bilayer. Once within the membrane, the hydrophobic helices of viroporins interact with each other to form higher ordered structures required to correctly perform their porating activities. This two-step process resembles the two-stage model proposed for membrane protein folding by Engelman and Poppot. In this review we use the membrane protein folding …
Nuevas perspectivas de análisis para entender la migración cualificada del sur de Europa hacia México
2018
This article focuses on skilled migration flows from Southern Europe into Mexico. From semi-structured interviews with Spanish and Italian immigrants living in four Mexican cities of Mexico, it addresses both reasons for immigration and labour incorporation in Mexican labour markets. Specifically, it explores: i) the role of the economic crisis and the lack of good employment in the countries of origin as a key factor when deciding an international migration; ii) the employment opportunities offered in Mexican labour markets for the highly-skilled immigrants, and iii) the role played by the Mexican state and its current immigration law when channelling this group into the labour market
Crystallization and preliminary crystallographic analysis of the major capsid proteins VP16 and VP17 of bacteriophage P23-77.
2012
The major capsid proteins VP16 and VP17 of bacteriophage P23-77 have been crystallized using both recombinant and purified virus and preliminary diffraction analyses have been performed.
Algu aprēķina moduļa uzlabojumi personālvadības sistēmā
2018
Kvalifikācijas darba “Algu aprēķina moduļa uzlabojumi personālvadības sistēmā” ietvaros tika veikti uzlabojumi uzņēmuma personālvadības sistēmas algu aprēķina modulī. Uzlabojumi sevī ietver gan kļūdu labojumus, gan jaunas funkcionalitātes ieviešanu, lai uzlabotu lietotāju pieredzi, lietojot sistēmu, un atvieglotu izstrādes procesu izstrādātājiem. Risinājuma izstrādē tika lietoti šādi rīki: JavaScript, TypeScrypt, HTML, CSS, Polymer Web Components, C#, KnockoutJS, “JetBrains Rider”, “MS Visual Studio Code”, GIT, TeamCity, Bitbucket.
Sphingomyelin induces structural alteration in canine parvovirus capsid
2008
One of the essential steps in canine parvovirus (CPV) infection, the release from endosomal vesicles, is dominated by interactions between the virus capsid and the endosomal membranes. In this study, the effect of sphingomyelin and phosphatidyl serine on canine parvovirus capsid and on the phospholipase A2 (PLA2) activity of CPV VP1 unique N-terminus was analyzed. Accordingly, a significant (P ≤ 0.05) shift of tryptophan fluorescence emission peak was detected at pH 5.5 in the presence of sphingomyelin, whereas at pH 7.4 a similar but minor shift was observed. This effect may relate to the exposure of VP1 N-terminus in acidic pH as well as to interactions between sphingomyelin and CPV. When…
Rīgas urbānās struktūras izmaiņas un reģenerācijas iespējas: Āgenskalna piemērs
2019
Pēdējos gados Rīgā attīstījies apkaimju aktīvisms. Šajā procesā iesaistījies arī Āgenskalns ar izveidotu apkaimes biedrību, taču apkaime piedzīvojusi arī pilsētas līmenī nozīmīgas pārmaiņas, tādas kā Āgenskalna tirgus nonākšanu privātā sektora nomnieku pārvaldībā. Bakalaura darba mērķis ir izvērtēt Āgenskalna centra urbānās struktūras izmaiņas un teritorijas potenciālu reģenerācijas pasākumu īstenošanai. Secināts, ka šobrīd nozīmīgākās urbānās struktūras izmaiņas norisinās tirdzniecības sektorā. Teritorijas reģenerācijas potenciāls ir augsts, to īpaši izceļ aktīvā kopiena, kā arī apbūves struktūra. Bakalaura darba noslēgumā tiek izvirzīti konkrēti priekšlikumi Āgenskalna centra reģenerācija…
Risupadot, putoukset ja tierummut taimenen (Salmo trutta) vaellusesteinä pienissä virtavesissä : kotiutumiskäyttäytymiseen perustuva tutkimus
2015
Virtavesissä kalojen liikkumista saattavat rajoittaa luonnostaan muodostuneet tai ihmisen rakentamat vaellusesteet. Muun muassa tienrakennuksen yhteydessä asennetut tierummut voivat estää kalojen kulun osittain tai kokonaan. Kivien tai kallion aiheuttama putous tai virran mukana kulkeutuva puuaines voivat myös synnyttää luonnollisen vaellusesteen. Suomessa vaellusesteitä on kartoitettu jonkin verran, mutta varsinaista tutkimusta on tehty vähän. Tässä tutkimuksessa tutkittiin, toimivatko risupadot, pienet putoukset ja tierummut taimenen (Salmo trutta) vaellusesteenä. Taimenet (91–383 mm) merkittiin mustepisteillä, Carlin-merkein tai rasvaeväleikkauksin ja siirrettiin esteen yläpuolelta sen a…
The Putative Metal Coordination Motif in the Endonuclease Domain of Human Parvovirus B19 NS1 Is Critical for NS1 Induced S Phase Arrest and DNA Damage
2012
The non-structural proteins (NS) of the parvovirus family are highly conserved multi-functional molecules that have been extensively characterized and shown to be integral to viral replication. Along with NTP-dependent helicase activity, these proteins carry within their sequences domains that allow them to bind DNA and act as nucleases in order to resolve the concatameric intermediates developed during viral replication. The parvovirus B19 NS1 protein contains sequence domains highly similar to those previously implicated in the above-described functions of NS proteins from adeno-associated virus (AAV), minute virus of mice (MVM) and other non-human parvoviruses. Previous studies have show…
TLR7 controls VSV replication in CD169(+) SCS macrophages and associated viral neuroinvasion
2019
Vesicular stomatitis virus (VSV) is an insect-transmitted rhabdovirus that is neurovirulent in mice. Upon peripheral VSV infection, CD169+ subcapsular sinus (SCS) macrophages capture VSV in the lymph, support viral replication, and prevent CNS neuroinvasion. To date, the precise mechanisms controlling VSV infection in SCS macrophages remain incompletely understood. Here, we show that Toll-like receptor-7 (TLR7), the main sensing receptor for VSV, is central in controlling lymph-borne VSV infection. Following VSV skin infection, TLR7−/− mice display significantly less VSV titers in the draining lymph nodes (dLN) and viral replication is attenuated in SCS macrophages. In contrast to effects o…