Search results for "VAST"
showing 10 items of 918 documents
Houkutteleva rekrytointiviestintä organisaation verkkosivuilla
2010
Nykyään organisaatioiden verkkosivut ovat pääasiallinen kanava työntekijöiden rekrytoinnissa. Tutkimuksen tarkoituksena oli selvittää millaista organisaatioiden rekrytointiviestintä on verkkosivujen rekrytointiosioissa. Työn tavoitteena oli myös kehittää rekrytointiviestinnän malli. Tutkimuskysymykset olivat 1) millaista organisaatioinformaatiota verkkosivujen rekrytointiosiot sisältävät ja 2) millaisia ovat työntekijöiden tarinat rekrytointiosioissa. Tutkimus keskittyi tarkastelemaan rekrytointiprosessin alkuvaiheen viestintää. Tutkimusaineisto koostui Suomen parhaat työpaikat -listan organisaatioiden verkkosivujen rekrytointiosioista. Kohdeorganisaatioiden rekrytointiosioiden sisältöä ana…
Justice in and to nature : an application of the broad framework of environmental and ecological justice
2017
This dissertation applies and develops the broad framework of environmental and ecological justice. It is a new relational approach to justice, whose elements have been introduced by David Schlosberg in his works on environmental and ecological justice. The present study provides a systematisation of the framework and applies it to contemporary environmental topics using the methods of conceptual analysis and case-implication critique. The main outcome of this study is that the elements comprising the broad framework of environmental and ecological justice provide fresh and useful insights into topics like species extinctions and ecosystem wellbeing. In particular, the holistic and conflict…
Silence and Resistance as Experiences and Presentations of Pupil Agency in Finnish Elementary School English Lessons
2014
This paper explores the multitude of ways in which Finnish fifth and sixth grade elementary school pupils experience and present their agency in English lessons, with a special focus on pupils' silence and resistance. Pupil agency is often seen as observable action that is oriented towards institutionally accepted goals and norms. In communicative and constructivist understandings of foreign language learning, learner agency is related to the idea of learning by participating. Participation in the classroom is not only individually but also socially constrained. This article is based on a larger, ecologically oriented ethnographic study on fifth and sixth graders' language learner identitie…
What Prevents Consumers from Making Responsible Online Purchases?
2023
The objective of this qualitative study is to examine and describe what prevents consumers from making responsible online purchases. The data were collected during February and March 2023 with an online survey. The data comprises the respondents' (N=245) free-form written responses, in which they articulated and explained the factors that prevent their responsible online purchases. The findings indicate that external and internal factors can prevent consumers' responsible online purchases. While external factors – online stores – are mainly blamed for promoting irresponsible buying behavior, internal factors –consumers' individual characteristics – are also recognized to have an important r…
Rho GTPases Are Involved in the Regulation of NF-κB by Genotoxic Stress
2001
A common cellular response to genotoxic agents and inflammatory cytokines is the activation of NF-kappaB. Here, we addressed the question of whether small GTPases of the Rho family are involved in the stimulation of NF-kappaB signaling by genotoxic agents or TNFalpha in HeLa cells. Inhibition of isoprenylation of Rho proteins by use of the HMG-CoA reductase inhibitor lovastatin attenuated UV-, doxorubicin-, and TNFalpha-induced degradation of IkappaBalpha as well as drug-stimulated DNA binding activity of NF-kappaB. Furthermore, NF-kappaB-regulated gene expression stimulated by either UV irradiation or treatment with TNFalpha was abrogated by lovastatin pretreatment. This indicates that iso…
Inhibition of small G proteins of the Rho family by statins orClostridium difficiletoxin B enhances cytokine-mediated induction of NO synthase II
2000
In order to investigate the involvement of Ras and/or Rho proteins in the induction of the inducible isoform of nitric oxide synthase (NOS II) we used HMG-CoA reductase inhibitors (statins) and Clostridium difficile toxin B (TcdB) as pharmacological tools. Statins indirectly inhibit small G proteins by preventing their essential farnesylation (Ras) and/or geranylgeranylation (Rho). In contrast, TcdB is a glucosyltransferase and inactivates Rho-proteins directly. Human A549/8- and DLD-1 cells as well as murine 3T3 fibroblasts were preincubated for 18 h with statins (1–100 μM) or TcdB (0.01–10 ng ml−1). Then NOS II expression was induced by cytokines. NOS II mRNA was measured after 4–8 h by R…
Inhibition of Protein Isoprenylation Impairs Rho-Regulated Early Cellular Response to Genotoxic Stress
2000
Activation of c-Jun N-terminal kinases (JNKs) and nuclear factor-kappaB (NF-kappaB) are early cellular responses to genotoxic stress involved in the regulation of gene expression. Pretreatment of cells with the hydroxymethyl glutaryl-CoA reductase inhibitor lovastatin blocked stimulation of JNK1 activity by UV irradiation and by treatment with the alkylating compound methyl methanesulfonate but did not affect activation of extracellular signal-regulated kinase 2 by UV light. Lovastatin also attenuated UV-induced degradation of the NF-kappaB inhibitor IkappaBalpha. The effects of lovastatin on UV-triggered stimulation of JNK1 as well as on IkappaBalpha degradation were reverted by cotreatmen…
Lovastatin inhibits Rho-regulated expression of E-selectin by TNFalpha and attenuates tumor cell adhesion.
2003
E-selectin mediated cell-cell adhesion plays an important role in inflammatory processes and extravasation of tumor cells. Tumor necrosis factor-alpha (TNF-alpha) induces E-selectin gene and protein expression in primary human endothelial cells (HUVEC) and in an endothelial cell line (EA.hy-926). As shown by ELISA and FACS analyses, HMG-CoA reductase inhibitors (e.g., lovastatin) impair the TNF-alpha stimulated increase in E-selectin protein expression. Similar results were obtained for E-selectin mRNA expression and promoter activity, indicating that the effect of lovastatin is based on inhibition of gene expression. The effective inhibitory concentration of lovastatin was in a physiologic…
Inhibition of Rho modulates cytokine-induced prostaglandin E2 formation in renal mesangial cells.
2003
Stimulation of rat mesangial cells for 24 h with interleukin-1β(IL-1β) plus forskolin (Fk) leads to a marked increase in prostaglandin E 2 (PGE 2 ) synthesis. This effect is further enhanced by the small G-protein Rho inhibitor toxin A. A similar increase in PGE 2 formation is obtained with Y27632, a Rho-dependent kinase inhibitor, and with lovastatin, a hydroxymethylglutaryl-coenzyme A inhibitor which depletes cells from geranylgeranyl moieties and thus blocks Rho activation. In parallel to the increased PGE 2 synthesis, a potentiation of IL-1β-induced secretory group IIA phospholipases A 2 (sPLA 2 -IIA) protein expression also occurs by Rho inhibition. However, only toxin A triggers an in…
REPORT SULLA CITTA' METROPOLITANA DI PALERMO
2016
Nell'ambito della riforma degli enti di area vasta il report fa il punto sulle criticità relative all'istituzione della Città metropolitana di Palermo.