Search results for "VECTORS"
showing 10 items of 601 documents
Biorthonormal-basis method for the vector description of optical-fiber modes
1998
This paper gives the theoretical basis for the development of real vector modal methods to describe optical-fiber modes. To this end, the vector wave equations, which determine the electromagnetic fields, are written in terms of a pair of linear, nonself-adjoint operators, whose eigenvectors satisfy biorthogonality relations. The key of our method is to obtain a matrix representation of the vector wave equations in a basis that is defined by the modes of an auxiliary system. Our proposed technique can be applied to fibers with any profile, even those with a complex refractive index. An example is discussed to illustrate our approach.
Improvement in Nuclear Entry and Transgene Expression of Baculoviruses by Disintegration of Microtubules in Human Hepatocytes
2005
ABSTRACT Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV), a potent virus for mammalian cell gene delivery, possesses an ability to transduce mammalian cells without viral replication. We examined the role of the cellular cytoskeleton in the cytoplasmic trafficking of viral particles toward the nucleus in human hepatic cells. Microscopic studies showed that capsids were found in the nucleus after either viral inoculation or cytoplasmic microinjection of nucleocapsids. The presence of microtubule (MT) depolymerizing agents caused the amount of nuclear capsids to increase. Overexpression of p50/dynamitin, an inhibitor of dynein-dependent endocytic trafficking from peripheral e…
Experimental validation of a general model for three phase inverters operating in healthy and faulty modes
2012
The paper presents the experimental verification of a general mathematical model of Voltage Source Inverters (VSI) able to simulate fault conditions and which is also useful for the simulation of fault-tolerant systems for different applications. In general, in the past, the problem of faulty inverters modeling has been addressed specifically by considering faults on the different phases as separate cases. Furthermore, traditional models include only the faulty mode and not the healthy mode, so resulting then not able to predict transient phenomena. The model hereafter presented overcomes this drawback. It was formulated by introducing the concept of “healthy leg binary variables”. Such var…
Controlled Fault-Tolerant Power Converters for Power Quality Enhancement
2013
Power quality depends generally on the interaction of electrical power with electrical equipments. If electrical equipments operate correctly and reliably without being damaged or stressed, a suitable level of power quality is assured. On the other hand, if the electrical equipment malfunctions, is unreliable, or is damaged during normal usage, power quality is poor and probably the economical loss could be important like the technical one. In the scenario of the Distributed Generation, power quality issues will be moreover important because an higher dissemination of power conditioning equipment will be requested and this obviously increases the sources of vulnerability of the electrical s…
Avidin Is a Promising Tag for Fusion Proteins Produced in Baculovirus-Infected Insect Cells
1999
The baculovirus expression vector system (BEVS) has become one of the most versatile and powerful eukaryotic systems for recombinant protein expression. We have constructed a novel baculovirus transfer vector (pbacAVs+C) which allows for the efficient production, detection, and single-step purification of the desired molecule as a secretion-compatible avidin fusion protein in insect cells. It also enables fast construction of the baculoviruses by site-specific transposition in Escherichia coli. To demonstrate the power of this vector, we report here on the production of immunologically intact hevein, a major cysteine-rich latex allergen, as avidin fusion protein. Our results indicate that a…
A new baseline for fascioliasis in Venezuela: lymnaeid vectors ascertained by DNA sequencing and analysis of their relationships with human and anima…
2011
Abstract Background Human and animal fascioliasis poses serious public health problems in South America. In Venezuela, livestock infection represents an important veterinary problem whereas there appear to be few human cases reported, most of which are passively detected in health centres. However, results of recent surveys suggest that the situation may be underestimated in particular areas. To obtain a baseline for future fascioliasis assessment, studies were undertaken by means of rDNA ITS-2 and ITS-1 and mtDNA cox 1 sequencing to clarify the specific status of Venezuelan lymnaeids, their geographical distribution and fascioliasis transmission capacity, by comparison with other American …
A Wickerhamomyces anomalus Killer Strain in the Malaria Vector Anopheles stephensi
2013
The yeast Wickerhamomyces anomalus has been investigated for several years for its wide biotechnological potential, especially for applications in the food industry. Specifically, the antimicrobial activity of this yeast, associated with the production of Killer Toxins (KTs), has attracted a great deal of attention. The strains of W. anomalus able to produce KTs, called "killer" yeasts, have been shown to be highly competitive in the environment. Different W. anomalus strains have been isolated from diverse habitats and recently even from insects. In the malaria mosquito vector Anopheles stephensi these yeasts have been detected in the midgut and gonads. Here we show that the strain of W. a…
New pegylated polyaspartamide-based polyplexes as gene delivery vectors
2010
Aims: To synthesize novel polyhydroxyethylaspartamide (PHEA) copolymers containing spermine (Spm) and polyethylene glycol (PEG) moieties in high yields, with the expectation that this material would show stealth properties and the ability to complex DNA by electrostatic interactions. Materials & methods: PHEA–PEG–Spm copolymer was prepared with a two-step reaction. Chemical, physicochemical and biological characterizations of PHEA–PEG–Spm copolymers and their obtained polyplexes with pDNA were performed. Results: The introduction of spermine in PHEA structure allows to obtain a copolymer bearing in the side chains polyamine moieties capable to interact with DNA. On the other hand, the …
A robust blind 3-D mesh watermarking based on wavelet transform for copyright protection
2019
Nowadays, three-dimensional meshes have been extensively used in several applications such as, industrial, medical, computer-aided design (CAD) and entertainment due to the processing capability improvement of computers and the development of the network infrastructure. Unfortunately, like digital images and videos, 3-D meshes can be easily modified, duplicated and redistributed by unauthorized users. Digital watermarking came up while trying to solve this problem. In this paper, we propose a blind robust watermarking scheme for three-dimensional semiregular meshes for Copyright protection. The watermark is embedded by modifying the norm of the wavelet coefficient vectors associated with th…
Kernel methods and their derivatives: Concept and perspectives for the earth system sciences.
2020
Kernel methods are powerful machine learning techniques which implement generic non-linear functions to solve complex tasks in a simple way. They Have a solid mathematical background and exhibit excellent performance in practice. However, kernel machines are still considered black-box models as the feature mapping is not directly accessible and difficult to interpret.The aim of this work is to show that it is indeed possible to interpret the functions learned by various kernel methods is intuitive despite their complexity. Specifically, we show that derivatives of these functions have a simple mathematical formulation, are easy to compute, and can be applied to many different problems. We n…