Search results for "VECTORS"
showing 10 items of 601 documents
Digenean parasites of the bivalve mollusc Pisidium amnicum in a small river in eastern Finland
1998
The host-parasite relationship between digeneans and a semelparous population of the mollusc. Pisidium amnicum Müller in a small river in eastern Finland was studied during 1992/1993. The parasite prevalence of the population was high. The total prevalence was 45.6% in 1992 (n = 790) and 47.5% in 1993 (n = 160). The dominant digenean, Bunodera luciopercae (34.2% in 1992, 35.0% in 1993), had highest prevalences in July/August and in winter. Two other species, Palaeorchis crassus (7.8% in 1992, 7.5% in 1993) and Phyllodistomum elongatum (4.7% and 5.0%), were rare during the winter. The prevalence of B. luciopercae increased as clams aged, while the other species were most common in middle-siz…
Yeast expression of the cytokine receptor domain of the soluble interleukin-6 receptor
1996
Abstract The complex of the soluble interleukin-6 receptor (sIL-6R) and IL-6 (IL-6) is a potent agonist on cells expressing the signal transducing protein gp130. In contrast, IL-6 alone only stimulates cells which express a membrane bound form of the IL-6R and gp130. The natural occurring sIL-6R is generated by shedding of the membrane receptor and to a lesser extend by alternative splicing. We have inserted the coding sequence of the 323 amino acid residues of the human sIL-6R into an expression/secretion vector suitable for the methylotrophic yeast Pichia pastoris . We obtained, however, no detectable expression and secretion of the recombinant protein. When we used only the coding sequen…
Complex formation between the NS3 serine-type proteinase of the hepatitis C virus and NS4A and its importance for polyprotein maturation
1995
Processing of the hepatitis C virus polyprotein is mediated by host cell signalases and at least two virally encoded proteinases. Of these, the serine-type proteinase encompassing the amino-terminal one-third of NS3 is responsible for cleavage at the four sites carboxy terminal of NS3. The activity of this proteinase is modulated by NS4A, a 54-amino-acid polyprotein cleavage product essential for processing at the NS3/4A, NS4A/4B, and NS4B/5A sites and enhancing cleavage efficiency between NS5A and NS5B. Using the vaccinia virus-T7 hybrid system to express hepatitis C virus polypeptides in BHK-21 cells, we studied the role of NS4A in proteinase activation. We found that the NS3 proteinase a…
Production of biologically active recombinant avidin in baculovirus-infected insect cells
1997
Abstract An efficient lepidopteran insect cell system was established for the expression of a recombinant form of chicken egg-white avidin. The gene product was obtained in both secreted and intracellular forms, and biologically active recombinant avidin was isolated using affinity chromatography on an iminobiotin–agarose column. Similar to the known quaternary structure of the native egg-white protein, the purified recombinant protein was glycosylated and assembled mainly into tetramers. Like native avidin, the recombinant tetramer also exhibited a high level of thermostability, and was further stabilized upon binding biotin. The biotin-binding and structural properties of the recombinant …
Sialotranscriptomics of the argasid tick ornithodoros moubata along the trophogonic cycle
2021
32 páginas, 8 tablas, 6 figuras
Insights in ChAdOx1 nCoV-19 vaccine-induced immune thrombotic thrombocytopenia
2021
Abstract SARS-CoV-2 vaccine ChAdOx1 nCoV-19 (AstraZeneca) causes a thromboembolic complication termed vaccine-induced immune thrombotic thrombocytopenia (VITT). Using biophysical techniques, mouse models, and analysis of VITT patient samples, we identified determinants of this vaccine-induced adverse reaction. Super-resolution microscopy visualized vaccine components forming antigenic complexes with platelet factor 4 (PF4) on platelet surfaces to which anti-PF4 antibodies obtained from VITT patients bound. PF4/vaccine complex formation was charge-driven and increased by addition of DNA. Proteomics identified substantial amounts of virus production-derived T-REx HEK293 proteins in the ethyle…
Deformed quons and bi-coherent states
2017
We discuss how a q-mutation relation can be deformed replacing a pair of conjugate operators with two other and unrelated operators, as it is done in the construction of pseudo-fermions, pseudo-bosons and truncated pseudo-bosons. This deformation involves interesting mathematical problems and suggests possible applications to pseudo-hermitian quantum mechanics. We construct bi-coherent states associated to $\D$-pseudo-quons, and we show that they share many of their properties with ordinary coherent states. In particular, we find conditions for these states to exist, to be eigenstates of suitable annihilation operators and to give rise to a resolution of the identity. Two examples are discu…
Finite-dimensional pseudo-bosons: a non-Hermitian version of the truncated harmonic oscillator
2018
We propose a deformed version of the commutation rule introduced in 1967 by Buchdahl to describe a particular model of the truncated harmonic oscillator. The rule we consider is defined on a $N$-dimensional Hilbert space $\Hil_N$, and produces two biorhogonal bases of $\Hil_N$ which are eigenstates of the Hamiltonians $h=\frac{1}{2}(q^2+p^2)$, and of its adjoint $h^\dagger$. Here $q$ and $p$ are non-Hermitian operators obeying $[q,p]=i(\1-Nk)$, where $k$ is a suitable orthogonal projection operator. These eigenstates are connected by ladder operators constructed out of $q$, $p$, $q^\dagger$ and $p^\dagger$. Some examples are discussed.
Two-dimensional Noncommutative Swanson Model and Its Bicoherent States
2019
We introduce an extended version of the Swanson model, defined on a two-dimensional noncommutative space, which can be diagonalized exactly by making use of pseudo-bosonic operators. Its eigenvalues are explicitly computed and the biorthogonal sets of eigenstates of the Hamiltonian and of its adjoint are explicitly constructed.We also show that it is possible to construct two displacement-like operators from which a family of bi-coherent states can be obtained. These states are shown to be eigenstates of the deformed lowering operators, and their projector allows to produce a suitable resolution of the identity in a dense subspace of \(\mathcal{L}^\mathrm{2}\, (\mathbb{R}^\mathrm{2})\).
Expression of B-Cell Activating Factor Enhances Protective Immunity of a Vaccine against Pseudomonas aeruginosa
2009
ABSTRACT B-cell activating factor (BAFF), a member of the TNF family, is a potent cytokine with stimulatory effects on B and T cells. To evaluate the potential of transient overexpression of BAFF to enhance vaccine immunogenicity, a replication-deficient adenovirus expressing full-length murine BAFF (AdBAFF) was tested in a mouse vaccine model against Pseudomonas aeruginosa . When coadministered with heat-killed P. aeruginosa , AdBAFF mediated a significant increase in anti- P. aeruginosa- specific serum and lung mucosal antibodies and resulted in improved protection against a lethal respiratory challenge with P. aeruginosa . This effect was independent of the site of administration of AdBA…