Search results for "VIRUS DISEASE"
showing 10 items of 1907 documents
Unusual Assortment of Segments in 2 Rare Human Rotavirus Genomes
2010
Using full-length genome sequence analysis, we investigated 2 rare G3P[9] human rotavirus strains isolated from children with diarrhea. The genomes were recognized as assortments of genes closely related to rotaviruses originating from cats, ruminants, and humans. Results suggest multiple transmissions of genes from animal to human strains of rotaviruses.
Homotypic Protection Against Rotavirus-Induced Diarrhea in Infant Mice Breast-Fed by Dams Immunized with the Recombinant VP8* Subunit of the VP4 Caps…
2000
The outer capsid proteins VP4 and VP7 induce neutralizing antibody against rotavirus. We have investigated in a mouse model the protection mediated by immunization with VP8*, the amino-terminal tryptic fragment of VP4. BALB/c female mice immunized with simian rotavirus SA11 VP6 and VP8* proteins expressed in Escherichia coli were mated with seronegative males. Litters were orally challenged with the SA11 strain (P5B[2], G3) or with the murine rotavirus strain EDIM (P10[16], G3) to verify the degree of protection against diarrhea induced in the newborns. Only those pups born to dams immunized with VP8* did not develop diarrhea after having been orally challenged with the SA11 strain. Pups bo…
Identification of a novel VP4 genotype carried by a serotype G5 porcine rotavirus strain
2006
AbstractRotavirus genome segment 4, encoding the spike outer capsid VP4 protein, of a porcine rotavirus (PoRV) strain, 134/04-15, identified in Italy was sequenced, and the predicted amino acid (aa) sequence was compared to those of all known VP4 (P) genotypes. The aa sequence of the full-length VP4 protein of the PoRV strain 134/04-15 showed aa identity values ranging from 59.7% (bovine strain KK3, P8[11]) to 86.09% (porcine strain A46, P[13]) with those of the remaining 25 P genotypes. Moreover, aa sequence analysis of the corresponding VP8* trypsin cleavage fragment revealed that the PoRV strain 134/04-15 shared low identity, ranging from 37.52% (bovine strain 993/83, P[17]) to 73.6% (po…
Rotavirus Genotypes in Sewage Treatment Plants and in Children Hospitalized with Acute Diarrhea in Italy in 2010 and 2011
2014
ABSTRACT Although the molecular surveillance network RotaNet-Italy provides useful nationwide data on rotaviruses causing severe acute gastroenteritis in children in Italy, scarce information is available on rotavirus circulation in the general Italian population, including adults with mild or asymptomatic infection. We investigated the genotypes of rotaviruses present in urban wastewaters and compared them with those of viral strains from clinical pediatric cases. During 2010 and 2011, 285 sewage samples from 4 Italian cities were tested by reverse transcription-PCRs (RT-PCRs) specific for rotavirus VP7 and VP4 genes. Rotavirus was detected in 172 (60.4%) samples, 26 of which contained mul…
Enteric calicivirus and rotavirus infections in domestic pigs
2009
SUMMARYWe report the prevalence of rotavirus and calicivirus infections, along with their respective association with diarrhoea in the porcine population of the region of northern Spain. A total of 221 samples were collected at random from different farms in the region and from the main slaughterhouse facility in the city of Zaragoza. Faecal samples were scored as diarrhoeic or normal and grouped into five groups to match general farm management and age criteria: group I (suckling 0–4 weeks), group II (weaning >4–8 weeks), group III (transition >8–16 weeks), group IV (fattening >16–24 weeks) and group V (adults >24 weeks). Group A rotavirus detection and caliciviruses were inves…
Genetic heterogeneity of porcine enteric caliciviruses identified from diarrhoeic piglets
2008
Enteric caliciviruses (noroviruses and sapoviruses) are responsible for the majority of non-bacterial gastroenteritis in humans of all age groups. Analysis of the polymerase and capsid genes has provided evidence for a huge genetic diversity, but the understanding of their ecology is limited. In this study, we investigated the presence of porcine enteric caliciviruses in the faeces of piglets with diarrhoea. A total of 209 samples from 118 herds were analysed and calicivirus RNA was detected by RT-PCR in 68 sample (32.5%) and in 46 herds (38.9%), alone or in mixed infection with group A and C rotaviruses. Sequence and phylogenetic analysis of the calicivirus-positive samples characterized t…
Demonstration and partial characterization of an intermediate HBcAG (Dane particle) population.
1981
Abstract Hepatitis-B core antigen (HBcAg) was released from Dane particles previously separated from anti-HBc by repeated pelleting through sucrose gradients separated into three HBcAg populations when analysed by cesium chloride density gradient centrifugation. Heavy HBcAg particles banded at a density of 1.355 gm/ml, intermediate HBcAg particles at a density of 1.33 gm/ml, and light mediate HBcAg particles at a density of 1.30 gm/ml. Like heavy HBcAg particles, intermediate HBcAg particles contained DNA polymerase activity, but the ratio of HBcAg to DNA polymerase activity was significantly different in both populations. Intermediate HBcAg particles could not be separated from heavy HBcAg…
The right to protection against poverty and social exclusion as a paradigm of respect for human dignity. The integration of the minimum vital income …
2020
La efectividad del derecho a la protección contra la pobreza y la exclusión social constituye la tarea más excelsa del jurista, tendente a conseguir el objetivo último de la Justicia y el respeto de la Dignidad Humana. Con tal premisa, en primer lugar, el autor defiende que tal derecho, por su carácter multifacético, ilustra de manera paradigmática el carácter indivisible de todos los derechos humanos. En segundo término, el artículo analiza la progresiva consolidación de los estándares internacionales (universales y europeos) en el ámbito de la erradicación de la pobreza y la exclusión social, poniendo el acento en el papel crucial del artículo 30 de la Carta Social Europea y de la jurispr…
The impact of the COVID-19 pandemic on human rights of women and girls with disabilities
2021
El presente artículo aborda la situación de especial vulnerabilidad en la que se encuentran las mujeres y niñas con discapacidad, la cual se ha visto agravada en el contexto de la crisis provocada por la pandemia de Covid-19. En efecto, en la primera parte el autor toma nota del actual deterioro de los derechos humanos de las mujeres y niñas con discapacidad, lo cual constituye un desafío para evitar su marginación en términos de doble discriminación. Para afrontar dicho reto, en la segunda parte se pone el énfasis en el papel crucial de la Convención de Naciones Unidas sobre los derechos de las personas con discapacidad como estándar mínimo de protección. En este sentido, en la tercera par…
Los efectos de la pandemia de COVID-19 en el ecosistema de festivales cinematográficos de la Comunitat Valenciana
2021
Este artículo analiza cómo ha afectado la pandemia de COVID-19 a los festivales de cine de la Comunitat Valenciana en un contexto de evolución forzada de este sector cultural a escala global. Para ello, se ha llevado a cabo, en primer lugar, un trabajo cuantitativo basado en una encuesta telemática enviada a la totalidad de festivales censados en el territorio valenciano. En segundo término, mediante la técnica de investigación cualitativa del grupo de discusión, se ha profundizado en los datos y conclusiones extraídas de la primera parte del estudio. Los principales resultados indican que, con variaciones respecto a las fechas habituales, reducción de aforos y programaciones, y la adopción…