Search results for "VITRO"

The use of morphokinetics as a predictor of embryo implantation.

2011

Background Time-lapse observation presents an opportunity for optimizing embryo selection based on morphological grading as well as providing novel kinetic parameters, which may further improve accurate selection of viable embryos. The objective of this retrospective study was to identify the morphokinetic parameters specific to embryos that were capable of implanting. In order to compare a large number of embryos, with minimal variation in culture conditions, we have used an automatic embryo monitoring system. Methods Using a tri-gas IVF incubator with a built-in camera designed to automatically acquire images at defined time points, we have simultaneously monitored up to 72 individual emb…

Design, Synthesis and Biological Evaluation of Novel Pyrazolo[1,2,4]triazolopyrimidine Derivatives as Potential Anticancer Agents

2021

Three novel pyrazolo-[4,3-e][1,2,4]triazolopyrimidine derivatives (1, 2, and 3) were designed, synthesized, and evaluated for their in vitro biological activity. All three compounds exhibited different levels of cytotoxicity against cervical and breast cancer cell lines. However, compound 1 showed the best antiproliferative activity against all tested tumor cell lines, including HCC1937 and HeLa cells, which express high levels of wild-type epidermal growth factor receptor (EGFR). Western blot analyses demonstrated that compound 1 inhibited the activation of EGFR, protein kinase B (Akt), and extracellular signal-regulated kinase (Erk)1/2 in breast and cervical cancer cells at concentrations…

L'intégration multisensorielle comme stratégie pour compenser la réduction en sodium et matière grasse dans les aliments

2015

In recent years;health authorities worldwide advise for a reduction of salt and fat in daily food consumption. However;foods with reduced salt and fat content are often not appreciated by consumers;Therefore;the formulation of low-salt-fat foods that maintain acceptability is a major concern in food research. In this thesis;the multi-sensory integration and release kinetics of flavor compounds were explored as strategies to compensate for salt and fat reduction in cheese products (model cheeses and real cheeses). The objective was to better understand the mechanisms leading to aroma and salt release during mastication and to evaluate how the matrix composition and structure influence salt a…

In vitro-Untersuchungen über den Einfluß von Digitoxin auf die Aufnahme von Calcium in Lipoidextrakte aus dem Herzmuskel / In-Vitro Studies of the Ef…

1969

In fruheren Versuchen an isolierten subcellularen Strukturen des Hundeherzens war eine Freisetzung von gebundenem Ca durch Herzglykoside beschrieben worden [3–5]. Da das cellular gebundene Ca vorwiegend in einer Bindung an die Lipoidkomponente der Membranstrukturen vorzuliegen scheint [1], wurde gepruft, ob Digitalis die Ca-Bindung an Lipoide zu beeinflussen vermag. Aus Meerschweinchenherzen wurden Mito-chondrien und Mikrosomen isoliert, die Lipoide extrahiert [2] und anschliesend nach der Methode von Nayler[6] die Aufnahme von Ca in diese Extrakte bei Aquilibrierung mit 45Ca-haltiger Tyrodelosung (1, 8 mM [Ca]) bestimmt. Bei Anwesenheit von Digitoxin wurde in einem Konzentrationsbereich vo…

Thioflavin T Promotes Aβ(1−40) Amyloid Fibrils Formation

2015

Fibrillogenesis of the small peptide Aβ(1-40) is considered to be the hallmark of Alzheimer's disease. Some evidence indicates small oligomers, rather than mature fibrils, as the key cytotoxic agents. The fluorescent dye Thioflavin T (ThT) is often used to detect amyloid deposits in both in vivo and in vitro experiments, and it is used to study kinetic measurements, under the fundamental hypothesis that this probe does not influence the aggregation processes. We report experimental data showing that ThT may promote the Aβ(1-40) peptide amyloid aggregation changing solvent-peptide interactions and stabilizing more ordered β-like conformation. This finding has a two-fold importance: It is a f…

Resveratrol: distribución, propiedades y perspectivas

2013

Resveratrol is a natural polyphenol which can be found in many plants and fruits, such as peanuts, mulberries, blueberries and, above all, in grapes and red wine. Its synthesis is regulated by the presence of stressful factors, such as fungal contamination and ultra-violet radiation. In plants, it plays a role as a phytoalexin, showing a capacity to inhibit the development of certain infections. Plant extracts which contain resveratrol have been employed by traditional medicine for more than 2000 years. Resveratrol was first isolated, and its properties were initially studied with scientific methods, thirty years ago. Its in vitro properties have been extensively studied and demonstrated. I…

In vitro effect of organophosphate pesticides, Malathion and chlorpyriphos, on lipid peroxidation and antioxidant enzymes

2008

Different metabolic behavior of long-chain n-3 polyunsaturated fatty acids in human platelets.

1988

Abstract Whereas numerous studies deal with the effects and metabolism of eicosapentaenoic acid (20:5( n −3)) in platelets, very few concern docosahexaenoic acid (22:6( n −3)), although both acids are consumed in equal amounts from most fish fat. The present paper reports the modulation of 22:6( n −3) oxygenation as well as that of endogenous arachidonic acid (20:4( n −6)) in 22:6( n −3)-rich platelets. Like the oxygenation of 20:5( n −3), the lipoxygenation of 22:6( n −3) occurred at a low level when incubated alone, but was markedly increased in the presence of 20:4( n −6), suggesting a similar peroxide tone dependency. 20:5( n −3) could not replace 20:4( n −6) in the increasing 22:6( n −…

Degradation of Acetylenic Triacylglyceroles and the Inactivation of Membrane Preparations from Moss Protonema Cells

1997

Protonema cells of the moss Ceratodon purpureus accumulate triacylglycerols with two acetylenic fatty acids, 9,12-octadecadien-6-ynoic acid (18:2A) and 9,12,15-octadeca-trein-6-ynoic acid (18:3A), as main components. By following the incorporation of the [14C]-precursors acetate, linoleate, α-linolenate, γ-linolenate, stearidonate and 18:2A, into 18:3A in triacylglycerol accumulating cells, the pathway for acetylenic acids could be established. 18:2A and 18:3A could be synthesized by a second desaturation of the Δ6 double bond of γ-linolenate and stearidonate, respectively. However the major pathway for 18:3A synthesis was via a Δ15 desaturation of 18:2A. Since 18:2A was found exclusively i…

Synthetic single and double aza-scorpiand macrocycles acting as inhibitors of the antioxidant enzymes iron superoxide dismutase and trypanothione red…

2014

The anti-chagasic activity of a series of eleven derivatives of aza-scorpiand-like macrocycles, some of them newly synthesised, was assayed. The four compounds with the best selectivity indices in vitro were subjected to in vivo assays. Tests in a murine model of the acute phase of Chagas disease showed a two-fold reduction in parasitaemia compared to that with benznidazole. Furthermore, compounds 7 and 11, with 4-pyridine and phenanthroline substituents in the lateral chain, caused a remarkable decrease in parasitaemia reactivation during the chronic phase after inducing immunosuppression in mice. These activity studies were complemented by measuring their inhibitory effect towards the ant…