Search results for "VITRO"

IN VITRO MODELS OF BLOOD-BRAIN BARRIER FORMATION AND FUNCTIONING IN MOLECULAR BASES OF NEURODEGENERETION

2005

In vitro models of blood-brain barrier and application in the study of the multiple sclerosis

2008

Vietējās kaņepju šķirnes „Pūriņi” augu ploiditātes un genotipu atšķirības molekulāro marķieru un eļļas specifisko īpašību nosakošo gēnu līmenī

2015

Sējas kaņepes (Cannabis sativ, L.) ir lauksaimniecībā nozīmīgs kultūraugs. Eiropas Savienības valstīs, arī Latvijā strauji pieaug interese par kaņepju audzēšanu. Pašlaik Latvijā nav savas sējas kaņepju šķirnes, kas būtu piemērotas sēklu iegūšanai. Jaunu šķirņu ātrākai iegūšanai ļoti nozīmīgas ir in vitro metodes, molekulāro metodžu izmantošana un iekļaušana selekcijas shēmā, tādēļ šim darbam ir sekojošs mērķis: vietējo kaņepju ‘Pūriņi’ in vitro kultivēšana, augu ģenētiskās daudzveidības un ploiditātes noteikšana, metilēšanas noteikšanas metodes izstrāde Δ12 un Δ15 desaturāzes gēnam. Veicot molekulāri ģenētisko analīzi izmantojot iPBS metodi konstatēts, ka vietējās kaņepes ‘Puriņi’ populācij…

The determination of the initial straight length in root canals of mandibular premolars--an in vitro study.

2009

Abstract The aim of the present roentgenographic in vitro study was to determine the initial straight length from the cemento-enamel junction (CEJ) to the appearance of a root canal curvature in human mandibular premolars. A total number of 282 mandibular premolars were examined. Exclusion criteria comprised root caries, extensive restorations and endodontically treated teeth. The teeth were fixed and digitally radiographed by means of a specially developed fixation device with standardized and reproducible distances with the parallel technique (Heliodent MD; Merlin 2.1). The distances from the CEJ to the first curvature (> 5°) (distance I), from the first curvature to a second curvature (d…

Innovative ultrasound systems for preclinical studies: design and production of new instruments and set-ups for in vitro and in vivo experiments – Si…

Tolerance and M2 (alternative) macrophage polarization are related processes orchestrated by p50 nuclear factor {kappa}B.

2009

Cells of the monocyte-macrophage lineage play a central role in the orchestration and resolution of inflammation. Plasticity is a hallmark of mononuclear phagocytes, and in response to environmental signals these cells undergo different forms of polarized activation, the extremes of which are called classic or M1 and alternative or M2. NF-kappaB is a key regulator of inflammation and resolution, and its activation is subject to multiple levels of regulation, including inhibitory, which finely tune macrophage functions. Here we identify the p50 subunit of NF-kappaB as a key regulator of M2-driven inflammatory reactions in vitro and in vivo. p50 NF-kappaB inhibits NF-kappaB-driven, M1-polariz…

Viewpoint: On the hysteresis in the human Achilles tendon

2013

NG-monomethyl-L-arginine and NG-nitro-L-arginine inhibit endothelium-dependent relaxations in human isolated omental arteries.

1991

Abstract The L-arginine analogues NG-monomethyl-l-arginine (l-NMMA, 10−4  m) and NG-nitro-L-arginine methyl ester (l-NAME, 10−4  m), which specifically inhibit the synthesis of nitric oxide from l-arginine, significantly reduced acetylcholine-induced endothelium-dependent relaxations in rings of human omental arteries. The inhibitory potency of l-NMMA and l-NAME was similar. Addition of l-NMMA or l-NAME to the organ bath did not induce any significant changes in the resting tension of the tissues. The effects of l-NMMA were reversed by l-arginine (3 × 10−4  m). The l-NMMA enantiomer, d-NMMA (10−4  m), did not influence either the basal tone of the preparation or the relaxing effects of acet…

Properties and significance of apoFNR as a second form of air-inactivated [4Fe-4S]·FNR of Escherichia coli

2005

The active form of the oxygen sensor fumarate nitrate reductase regulator (FNR) of Escherichia coli contains a [4Fe-4S] cluster which is converted to a [2Fe-2S] cluster after reaction with air, resulting in inactivation of FNR. Reaction of reconstituted [4Fe-4S].FNR with air resulted within 5 min in conversion to apoFNR. The rate was comparable to the rate known for [4Fe-4S].FNR/[2Fe-2S].FNR cluster conversion, suggesting that apoFNR is a product of [2Fe-2S].FNR decomposition and a final form of air-inactivated FNR in vitro. Formation of apoFNR and the redox state of the cysteinyl residues were determined in vitro by alkylation. FNR contains five cysteinyl residues, four of which (Cys20, Cy…

Prediction of properties of chiral compounds by molecular topology

1998

Abstract A common assumption in chemistry is that chiral behavior is associated with 3-D geometry. However, chiral information is related to symmetry, which allows the topological handling of chiral atoms by weighted graphs and the calculation of new descriptors that give a weight to the corresponding entry in the main diagonal of the topological matrix. In this study, it is demonstrated that, operating in this way, chiral topological indices are obtained that can differentiate the pharmacological activity between pairs of enantiomers. The 50% inhibitory concentration (IC50) values of the D2 dopamine receptor and the σ receptor for a group of 3-hydroxy phenyl piperidines are specifically pr…