Search results for "Vector"

Baculovirus-mediated periadventitial gene transfer to rabbit carotid artery

2000

Recombinant Autographa californica multiple nuclear polyhedrosis viruses (AcMNPV) have recently been shown to transduce mammalian cells in vitro. Since baculoviruses offer many advantages over viruses currently used in gene therapy, we have tested them for in vivo gene transfer by constructing a baculovirus bearing a nuclear targeted beta-galactosidase marker gene (LacZ) under a CMV promoter. Both rabbit aortic smooth muscle cells (RAASMC) and human ECV-304 cells were susceptible to LacZ-baculovirus transduction. Transgene expression was evaluated in vivo by applying 1 x 10(9) p.f.u. of LacZ-baculoviruses or LacZ-adenoviruses in a silastic collar placed around rabbit carotid arteries in the…

Norman Graphics: A Multimedia Manifesto

2020

The apses of the Cathedral of Monreale (XII century) are characterised by extraordinarily rich decorations, to enhance the most sacred part of the sanctuary where the altar is located. The paper describes some circular inlays of the central apse of the Cathedral of Monreale through a geometric analysis communicated via a multimedia language. The geometric motifs can be arranged in a graphic composition according to an imaginative process that collects all the scientific analysis procedure described above such as a sort of Manifesto of the Norman architectural culture geometry in Sicily and in southern Italy. The Manifesto is an audio-visual product, consisting of vector drawings’ animations…

Weakly supervised alignment of multisensor images

2015

Manifold alignment has become very popular in recent literature. Aligning data distributions prior to product generation is an appealing strategy, since it allows to provide data spaces that are more similar to each other, regardless of the subsequent use of the transformed data. We propose a methodology that finds a common representation among data spaces from different sensors using geographic image correspondences, or semantic ties. To cope with the strong deformations between the data spaces considered, we propose to add nonlineari-ties by expanding the input space with Gaussian Radial Basis Function (RBF) features with respect to the centroids of a partitioning of the data. Such featur…

The beer market and advertising expenditure

2009

PurposeThe purpose of this paper is to examine the impacts of advertising expenditure on brands' market shares, utilizing a novel four‐week advertising‐sales data from the highly competitive oligopolistic Finnish beer market in which price competition among the homogeneous larger‐type beer brands is not allowed during the period of the study.Design/methodology/approachCompetition is modelled using the Lanchester model. The impacts of advertising on market shares are estimated using the impulse‐response functions from vector autoregression, and the full information maximum likelihood and advertising elasticities.FindingsSome new insights into beer market dynamics are obtained. First, the imp…

Assessing the Impact of Temporary Retail Price Discounts Intervals Using SVM Semiparametric Regression

2009

Although the marketing literature has found that temporary retail price discounts cause a significant sales increase, little is known about the specific characteristics of deals that influence the magnitude of the sales spike. In this paper, we analyse the impact of the length of temporary retail price discounts periods on the sales increase using scanner-store daily-sales data for two frequently purchased product categories: ground coffee (a storable category) and yogurt (a perishable category).Wedevelop a robust semiparametric regression model based on support vector statistical theory with several previously proposed predictors along with a daily time description. This model also makes i…

Using Support Vector Semiparametric Regression to estimate the effects of pricing on brand substitution

2008

Infilled frames: developments in the evaluation of the stiffening effect of infills

2003

In order to consider the modified seismic response of framed structures in the presence of masonry infills, proper models have to be formulated. Because of the complexity of the problem, a careful definition of a diagonal pin-jointed strut, able to represent the horizontal force-interstorey displacement cyclic law of the actual infill, may be a solution. In this connection the present paper shows a generalized criterion for the determination of the ideal cross-section of the strut mentioned before. The procedure is based on the equivalence between the lateral stiffness of the actual infilled frame scheme during the conventional elastic stage of the response and the lateral stiffness of the …

Photoinduced mass transfer in amorphous As 2 S 3 films

2011

The surface relief grating formation in amorphous As2S3 films strongly depends on the polarization state of recording beams. The surface relief grating formation efficiency of s-s and p-p recording beam combination can be essentially enhanced by additional illumination with orthogonal polarization. It is shown that the direction of mass transport on the film surface is determined by the direction of light electric vector (© 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

A New Iterative Procedure for the Localization of a Moving Object/Person in Indoor Areas from Received RF Signals

2019

This paper presents a new iterative estimation method to localize a single moving object or person in non-stationary 3-dimensional (3D) indoor environments from received radiofrequency (RF) signals. The moving object/person is modelled by a moving single point scatterer. The indoor space is equipped with a multiple-input multiple-output (MIMO) communication system. This work starts by introducing a new geometrical channel model which considers the effects of the line-of-sight (LOS) component, the fixed objects located in a room, and the moving object (point scatterer). Then, we present an iterative estimation technique for computing the time-variant (TV) coordinates of the moving scatterer.…

A New Hyaluronic Acid Derivative Obtained from Atom Transfer Radical Polymerization as a siRNA Vector for CD44 Receptor Tumor Targeting.

2015

Two derivatives of hyaluronic acid (HA) have been synthesized by atom transfer radical polymerization (ATRP), starting from an ethylenediamino HA derivative (HA-EDA) and by using diethylaminoethyl methacrylate (DEAEMA) as a monomer for polymerization. Both samples, indicated as HA-EDA-pDEAEMA a and b, are able to condense siRNA, as determined by gel retardation assay and resulting complexes show a size and a zeta potential value dependent on polymerization number, as determined by dynamic light scattering measurements. In vitro studies performed on HCT 116 cell line, that over express CD44 receptor, demonstrate a receptor mediated uptake of complexes, regardless of their surface charge. New…