Search results for "Vector"
showing 10 items of 2660 documents
Regeneration of total RNA purification silica-based columns
2010
Silica-based columns are largely used in RNA purification, allowing fast extractions and good yields of high quality nucleic acid, but their major limitation is the high cost. The reuse of such columns, although desirable, is not recommended because of residual amounts of material from the previous sample trapped in the column matrix, which might be released during further purification. Thus, recycling does need previous complete removal of any detectable RNA trace, but to date no protocol which allows decontamination and reuse is available.We report a very rapid decontamination procedure, based on treatment with warm alkaline solution containing Triton X-100, which ensures no RNA carry-ove…
Comment on "Displacement along the Karakoram fault, NW Himalaya, estimated from LA-ICP-MS U Pb dating of offset geologic markers" published by Shifen…
2013
International audience; Field evidence for syn-deformation migmatization and crystallization along the Karakorum fault contradicts the study of Wang et al. (2012). The ages of such magmatic rocks provide minimum ages for the onset of deformation at similar to 23 Ma in North Ayilari and similar to 19 Ma in Tangtse. The onset of deformation at 12 Ma in the Ayilari range inferred by Wang et al. (2012) from a muscovite Ar/Ar age is a cooling age, thus only a minimum age. The similar to 60 Ma granodiorite bodies, the ophiolitic rocks and the south Kailash thrust that are correlated across the fault to provide a 52 +/- 2 km finite offset do not define reliable piercing points. Such observations a…
Adenovirus-mediated wild-type p53 gene transfer in patients receiving chemotherapy for advanced non-small-cell lung cancer: results of a multicenter …
2001
PURPOSE: To study the additional benefit from adenoviral p53 gene therapy in patients undergoing first-line chemotherapy for advanced non–small-cell lung cancer (NSCLC). PATIENTS AND METHODS: Twenty-five patients with nonresectable NSCLC were enrolled in an open-label, multicenter phase II study of three cycles of regimen A, carboplatin (area under the curve, 6; day 1) plus paclitaxel (175 mg/m2, day 1), or regimen B, cisplatin (100 mg/m2, day 1) plus vinorelbine (25 mg/m2, days 1, 8, 15, and 22) in combination with intratumoral injection of 7.5 × 1012 particles of SCH 58500 (rAd/p53, day 1). Responses of individual tumor lesions were assessed after each cycle, and gene transfer was examin…
Covarying patterns of white matter lesions and cortical atrophy predict progression in early MS
2020
ObjectiveWe applied longitudinal 3T MRI and advanced computational models in 2 independent cohorts of patients with early MS to investigate how white matter (WM) lesion distribution and cortical atrophy topographically interrelate and affect functional disability.MethodsClinical disability was measured using the Expanded Disability Status Scale Score at baseline and at 1-year follow-up in a cohort of 119 patients with early relapsing-remitting MS and in a replication cohort of 81 patients. Covarying patterns of cortical atrophy and baseline lesion distribution were extracted by parallel independent component analysis. Predictive power of covarying patterns for disability progression was tes…
Gold Nanoparticle-Assisted Virus Formation by Means of the Delivery of an Oncolytic Adenovirus Genome
2020
[EN] Oncolytic adenoviruses are a therapeutic alternative to treat cancer based on their ability to replicate selectively in tumor cells. However, their use is limited mainly by the neutralizing antibody (Nab) immune response that prevents repeated dosing. An alternative to facilitate the DNA access to the tumor even in the presence of anti-viral Nabs could be gold nanoparticles able to transfer DNA molecules. However, the ability of these nanoparticles to carry large DNA molecules, such as an oncolytic adenovirus genome, has not been studied. In this work, gold nanoparticles were functionalized with different amounts of polyethylenimine to transfer in a safe and efficient manner a large on…
Analytical and experimental evaluation of a WECS based on a Doubly Fed Induction Generator fed by a matrix converter
2008
In this paper the control of a grid-connected Wind Energy Conversion System (WECS), based on a sensorless vector controlled Doubly-Fed Induction Generator (DFIG) fed by a matrix converter, is presented. The matrix converter is controlled using a space vector modulation algorithm. Stability issues related to the operation of the WECS connected to the grid using a matrix converter are also discussed in this work. The influence of a synchronous rotating filter in the dynamic of the proposed WECS is analysed. A Model Reference Adaptive System (MRAS) observer for sensorless control of the proposed WECS is used in this work. Using the speed estimated by the MRAS observer the electrical torque of …
Transparent boundary condition for acoustic propagation in lined guide with mean flow
2008
A finite element analysis of acoustic radiation in an infinite lined guide with mean flow is studied. In order to bound the domain, transparent boundary conditions are introduced by means of a Dirichlet to Neumann (DtN) operator based on a modal decomposition. This decomposition is easy to carry out in a hard‐walled guide. With absorbant lining, many difficulties occur even without mean flow. Since the eigenvalue problem is no longer selfadjoint, acoustic modes are not orthogonal with respect to the L2‐scalar product. However, an orthogonality relation exists which permits writing the modal decomposition. For a lined guide with uniform mean flow, modes are no longer orthogonal but a new sca…
Eigenvectors of k–ψ-contractive wedge operators
2008
Abstract We present new boundary conditions under which the fixed point index of a strict- ψ -contractive wedge operator is zero. Then we investigate eigenvalues and corresponding eigenvectors of k – ψ -contractive wedge operators.
IOWA Operators and Its Application to Image Retrieval
2014
This paper presents a relevance feedback procedure based on logistic regression analysis. Since, the dimension of the feature vector associated to each image is typically larger than the number of evaluated images by the user, different logistic regression models have to be fitted separately. Each fitted model provides us with a relevance probability and a confidence interval for that probability. In order to aggregate these set of probabilities and confidence intervals we use an IOWA operator. The results will show the success of our algorithm and that OWA operators are an efficient and natural way of dealing with this kind of fusion problems.
The Probability Law for Generic Density Operators
2020
In this chapter, the probability law of the non-null eigenstates of a generic density operator—studied in the previous chapter—is determined, by showing that given the composite system and the subsystem being considered, a mapping arises which associates a universal probability distribution to the non-null eigenstates of the generic density operator. We thus recover the Born statistical interpretation without having assumed it as a postulate.