Search results for "Vertebra"
showing 10 items of 1220 documents
Specific binding of Bacillus thuringiensis Cry2A insecticidal proteins to a common site in the midgut of Helicoverpa species
2008
ABSTRACT For a long time, it has been assumed that the mode of action of Cry2A toxins was unique and different from that of other three-domain Cry toxins due to their apparent nonspecific and unsaturable binding to an unlimited number of receptors. However, based on the homology of the tertiary structure among three-domain Cry toxins, similar modes of action for all of them are expected. To confirm this hypothesis, binding assays were carried out with 125 I-labeled Cry2Ab. Saturation assays showed that Cry2Ab binds in a specific and saturable manner to brush border membrane vesicles (BBMVs) of Helicoverpa armigera . Homologous-competition assays with 125 I-Cry2Ab demonstrated that this toxi…
Lack of Cry1Fa binding to the midgut brush border membrane in a resistant colony of Plutella xylostella moths with a mutaton in the ABCC2 locus
2012
ABSTRACT Previous studies reported “mode 1” Bacillus thuringiensis resistance in a colony of diamondback moths (NO-QA), and recently, this resistance has been mapped to an ABC transporter ( ABCC2 ) locus. We report the lack of binding of Cry1Fa to insects derived from this colony and compare our data with those from other insects with ABCC2 -associated resistance.
Interaction of Bacillus thuringiensis Cry1 and Vip3A Proteins with Spodoptera frugiperda Midgut Binding Sites
2009
ABSTRACT Vip3Aa, Vip3Af, Cry1Ab, and Cry1Fa were tested for their toxicities and binding interactions. Vip3A proteins were more toxic than Cry1 proteins. Binding assays showed independent specific binding sites for Cry1 and Vip3A proteins. Cry1Ab and Cry1Fa competed for the same binding sites, whereas Vip3Aa competed for those of Vip3Af.
Assessing autophagy in archived tissue or how to capture autophagic flux from a tissue snapshot
2020
This article belongs to the Special Issue Autophagy in Cancer.
Specific binding of radiolabeled Cry1Fa insecticidal protein from Bacillus thuringiensis to midgut sites in lepidopteran species
2012
ABSTRACT Cry1Fa insecticidal protein was successfully radiolabeled with 125 I-Na. Specific binding to brush border membrane vesicles was shown for the lepidopteran species Ostrinia nubilalis , Spodoptera frugiperda , Spodoptera exigua , Helicoverpa armigera , Heliothis virescens , and Plutella xylostella . Homologous competition assays were performed to obtain equilibrium binding parameters ( K d [dissociation constant] and R t [concentration of binding sites]) for these six insect species.
Multivariate analysis of sites using water invertebrates and land use as indicators of the quality of biotopes of Mediterranean relic plant (Petagnae…
2007
Petagnaea gussonei is a rhizomatous, perennial scapose hemicryptophyte species belonging to a monospecific genus of Apiaceae. It lives only along woodland rivulets and is a Tertiary relic localized in the restricted area of the Nebrodi Mountains (NE Sicily, Italy). Ecological indicators have been employed to understand the status of eight localities (intermittent rivulets) where the species occurs. In order to evaluate both the rivulets' water-quality and landscape use, index of anthropisation (Ia), water fleas bioassay (TU), and analyses of water macroinvertebrates have been employed. The results obtained for the level of toxicity exclude the presence of macropollutants (TU < 0.001), where…
The fossil rodent faunas of the localities Alcoy 2C and 2D (Alcoy Basin, Spain). Implications for dating the classical locality of Alcoy-Mina
2014
In the Gormaig area (Alcoy basin, SE Spain) a number of fossil mammal localities are known, of which only the classic site of Alcoy-Mina, known since the mid-19th century, has yielded remains of macrofauna. In the vicinity of this site are the localities of Alcoy-2C (AL2C) and Alcoy-2D (AL2D), probably representing the levels of Alcoy-Mina. The faunal assemblage allows dating the AL2C and AL2D localities as Early Pliocene (end of the Early Ruscinian or early-late Ruscinian boundary, MN14-MN15). Several taxa (Pliopetaurista and Glis) are thought to have affinities for humid and close biotopes. En el área del Gormaig (cuenca de Alcoy, SE España), se conocen toda una serie de yacimientos de ma…
Development and characterization of an experimental model of diet-induced metabolic syndrome in rabbit
2017
Metabolic syndrome (MetS) has become one of the main concerns for public health because of its link to cardiovascular disease. Murine models have been used to study the effect of MetS on the cardiovascular system, but they have limitations for studying cardiac electrophysiology. In contrast, the rabbit cardiac electrophysiology is similar to human, but a detailed characterization of the different components of MetS in this animal is still needed. Our objective was to develop and characterize a diet-induced experimental model of MetS that allows the study of cardiovascular remodeling and arrhythmogenesis. Male NZW rabbits were assigned to control (n = 15) or MetS group (n = 16), fed during 2…
Disc degeneration and bone density in monozygotic twins discordant for insulin-dependent diabetes mellitus
2000
The effects of insulin-dependent diabetes mellitus on bone density and connective tissue degeneration have theoretical interest and practical relevance. Several experimental studies in animals have demonstrated the harmful effects of insulin deficiency on connective tissues. However, clinical studies in humans have produced somewhat contradictory results, most likely due to difficulties controlling for general degeneration and factors associated with diabetes. In nine pairs of monozygotic twins discordant for insulin-dependent diabetes mellitus, we compared femoral and lumbar bone mineral density (assessed by dual-energy x-ray absorptiometry) and spinal degeneration (assessed by magnetic re…
Quantitative ultrasound predicts bone mineral density and failure load in human lumbar vertebrae
2006
Quantitative ultrasound is in widespread clinical use for assessment of bone quality at peripheral skeletal sites, but has not yet been applied to those sites in the axial skeleton, such as the spine and hip, where osteoporotic fractures are common.Ultrasound measurements were made in 11 cadaveric vertebrae and relationships with bone mineral density and failure load were investigated. An ultrasonic imaging system was used to measure speed of sound, broadband ultrasonic attenuation, and attenuation at a single frequency, through the vertebral body in the sagittal plane. Ultrasonic measurements were averaged over a region of interest centrally within the vertebral body, and were calculated w…