Search results for "Vine"
showing 10 items of 2017 documents
Autotetraploid Emergence via Somatic Embryogenesis in Vitis vinifera Induces Marked Morphological Changes in Shoots, Mature Leaves, and Stomata
2021
Polyploidy plays an important role in plant adaptation to biotic and abiotic stresses. Alterations of the ploidy in grapevine plants regenerated via somatic embryogenesis (SE) may provide a source of genetic variability useful for the improvement of agronomic characteristics of crops. In the grapevine, the SE induction process may cause ploidy changes without alterations in DNA profile. In the present research, tetraploid plants were observed for 9.3% of ‘Frappato’ grapevine somatic embryos regenerated in medium supplemented with the growth regulators β-naphthoxyacetic acid (10 µM) and N6-benzylaminopurine (4.4 µM). Autotetraploid plants regenerated via SE without detectable changes in the …
Homozygote Depression in Gamete-Derived Dragon-Fruit (Hylocereus) Lines
2018
Putative gamete-derived progenies from two Hylocereus species, the diploid H. monacanthus and the tetraploid H. megalanthus, were studied with the dual aims to confirm their gamete origin and to evaluate their potential use as genetic resources. An additional goal was to determine the origin (allotetraploid vs. autotetraploid) of H. megalanthus by exploring morphological variations in the di-haploid (2x) H. megalanthus progeny. Gamete origin was proved in all five H. monacanthus lines obtained and in 49 of the 70 H. megalanthus lines by using flow cytometry and simple sequence repeat (SSR) markers. The five double-haploid (2x) H. monacanthus lines showed low vigor and abnormal flower develo…
Thioredoxin (Trxo1) interacts with proliferating cell nuclear antigen (PCNA) and its overexpression affects the growth of tobacco cell culture.
2017
Thioredoxins (Trxs), key components of cellular redox regulation, act by controlling the redox status of many target proteins, and have been shown to play an essential role in cell survival and growth. The presence of a Trx system in the nucleus has received little attention in plants, and the nuclear targets of plant Trxs have not been conclusively identified. Thus, very little is known about the function of Trxs in this cellular compartment. Previously, we studied the intracellular localization of PsTrxo1 and confirmed its presence in mitochondria and, interestingly, in the nucleus under standard growth conditions. In investigating the nuclear function of PsTrxo1 we identified proliferati…
Survey of five major grapevine viruses infecting Blatina and Žilavka cultivars in Bosnia and Herzegovina
2021
The sanitary status of grapevines has not yet been considered sufficiently in vineyards throughout Bosnia and Herzegovina (BiH). An extensive survey of five major grapevine viruses in the country was carried out in 2019. A total of 630 samples from the two dominant autochthonous cultivars, named Žilavka and Blatina, were tested by DAS-ELISA for the presence of grapevine leafroll-associated viruses (GLRaV-1 and 3), grapevine fleck virus (GFkV), grapevine fanleaf virus (GFLV) and Arabis mosaic virus (ArMV). Eighty-eight % of the samples were positive for at least one virus, and all five viruses were detected, thought with different incidence, i.e. GLRaV-3 (84%), GFLV (43%), GLRaV-1 (14%), GFk…
Rapid dissemination of Mycobacterium bovis from cattle dung to soil by the earthworm Lumbricus terrestris
2016
International audience; Indirect transmission of Mycobacterium bovis, the causative agent of bovine tuberculosis (bTB), between wildlife and livestock is thought to occur by inhalation or ingestion of environmental substrates contaminated through animal shedding. The role of the soil fauna, such as earthworms, in the circulation of M. bovis from contaminated animal feces is of interest in the epidemiology of bTB. The objective of this study was to assess the impact of earthworm activity on M. bovis transfer from animal dung to castings and the surrounding soil. For this purpose, microcosms of soil containing the anecic eathworms Lumbricus terrestris were prepared and covered with cattle fec…
Clone-Dependent Expression of Esca Disease Revealed by Leaf Metabolite Analysis
2019
International audience; Grapevine trutk diseases, especially Esca, are of major concern since they gradually alter vineyards worldwide and cause heavy economic losses. The expression of Esca disease symptoms depends on several factors, including the grapevine cultivar. In this context, a possible clone-dependent expression of the Esca disease was studied. Two clones of 'Chardonnay' grown in the same plot were compared according to their developmental and physiological traits, metabolome, and foliar symptom expression. Analysis of their leaf metabolome highlighted differences related to symptom expression. Interestingly, the content of a few specific metabolites exhibited opposite variations…
An ancestral allele of grapevine transcription factor MYB14 promotes plant defence
2016
Highlight The molecular mechanisms underlying the elevated inducibility of stilbene in pathogen-resistant Vitis sylvestris can be explained by the increased inducibility of the MYB14 promoter.
Lysimeter assessment of the Simplified Two-Source Energy Balance model and eddy covariance system to estimate vineyard evapotranspiration
2019
Abstract Estimation of crop water needs plays a key role in the water resource management in arid and semi-arid regions. Actual evapotranspiration (ETa) becomes the key term in both water and energy balances at this point. In this work we focus on vineyard due to the significance of this crop for La Mancha region, Spain, with the greatest concentration of vineyards in the world. Eddy-covariance (EC) technique has been traditionally used for ground observations of ETa. One of the aims of this work is to assess the feasibility of an EC system under the challenging conditions of a small drip-irrigated vineyard in a semi-arid environment. Two-source energy balance modelling allows for ETa estim…
Combined influence of bud load and bud position along the cane on vegetative and reproductive parameters of grape cv. Grillo
2019
Cultivar Grillo vines are characterized by problems with flower biology (the fertility of basal buds) and fructification (millerandage). In this study, to manage the variability in bunch weight with winter pruning and to program others canopy management practices (i.e. early defoliation), three different treatments of bud load were set up by leaving the cane with 3, 6 or 10 buds. The effects of bud load and cane length were studies regarding bud fertility, shoot leaf area, and the number of flowers and berries, as well as the relationship between leaf area and percentage of fruit set, leaf area/flower and percentage of fruit set, and the number of hens and chicks berries. Shoots in the dist…
Integrative effects of vine water relations and grape ripeness level of vitis vinifera L. cv. Shiraz/Richter 99. II. grape composition and wine quali…
2016
Regulation of grapevine water status is a common practice to manipulate grape composition and wine quality. In this investigation the effect of plant water status (two field water capacity-based irrigation levels, 75% and 100%, applied at single and combined vine developmental stages) and ripeness level (harvesting at different soluble solid levels) on grape composition and wine quality of Vitis vinifera L. cv. Shiraz/ Richter 99 was determined. Integrative effects of vine water relations and grape ripeness level, specifically in a Mediterranean high winter rainfall area, have not yet been investigated systematically. Source:sink mechanisms and dynamics and compositional and physical change…