Search results for "WES"

Receiver-operating characteristic curves for somatic cell scores and California mastitis test in Valle del Belice dairy sheep.

2013

Using receiver-operating characteristic (ROC) curve methodology this study was designed to assess the diagnostic effectiveness of somatic cell count (SCC) and the California mastitis test (CMT) in Valle del Belice sheep, and to propose and evaluate threshold values for those tests that would optimally discriminate between healthy and infected udders. Milk samples (n = 1357) were collected from 684 sheep in four flocks. The prevalence of infection, as determined by positive bacterial culture was 0.36, 87.7% of which were minor and 12.3% major pathogens. Of the culture negative samples, 83.7% had an SCC < 500,000/mL and 97.4% had <1,000,000 cells/mL. When the associations between SC score (SC…

Requirement of Retinoic Acid Receptor Isotypes α, β, and γ during the Initial Steps of Neural Differentiation of PCC7 Cells

2005

Retinoic acid (RA) is indispensable for morphogenesis and differentiation of several tissues, including the nervous system. The requirement of the RA receptor (RAR) isotypes alpha, beta, and gamma and the putative role of retinoid X receptor-(RXR) signaling in RA-induced neural differentiation, was analyzed. For this compound-selective retinoids and the murine embryonal carcinoma cell line PCC7, a model system for RA-dependent neural differentiation was used. The present paper shows that proliferating PCC7 cells primarily express RXRalpha and RARalpha, lower levels of RXRbeta, and barely detectable amounts of RARbeta, RARgamma, and RXRgamma. At receptor-selective concentrations, only a RARa…

Antagonistic feedback loops involving Rau and Sprouty in the Drosophila eye control neuronal and glial differentiation.

2013

During development, differentiation is often initiated by the activation of different receptor tyrosine kinases (RTKs), which results in the tightly regulated activation of cytoplasmic signaling cascades. In the differentiation of neurons and glia in the developing Drosophila eye, we found that the proper intensity of RTK signaling downstream of fibroblast growth factor receptor (FGFR) or epidermal growth factor receptor required two mutually antagonistic feedback loops. We identified a positive feedback loop mediated by the Ras association (RA) domain-containing protein Rau that sustained Ras activity and counteracted the negative feedback loop mediated by Sprouty. Rau has two RA domains t…

Myślenie dialogiczne. W polu gry o prawdę etyczną

2019

Przedmiotem analiz artykułu jest pojęcie myślenia dialogicznego. Jest ono różne od pojęcia dialogu – literackiej figury, która służy do przekazywania poglądów filozoficznych. Myślenie dialogiczne, jako merytorycznie nowy sposób uprawiania filozofii, pojawia się dopiero w początkach XX wieku. Zrodziło się ono jako sprzeciw wobec redukcyjnych metod nauk przyrodniczych i przeniesienia ich w obszar myśli humanistycznej i antropologicznej. W ten sposób powstał nowy nurt filozoficzny – filozofia dialogu. Rozwijał się w duchu uwag krytycznych kierowanych pod adresem dotychczasowej tradycji filozofii zachodniej. W artykule przedstawione zostały konstytutywne cechy myślenia dialogicznego. Najpierw p…

Hamster Bcl-2 Protein Is Cleaved in Vitro and in Cells by Caspase-9 and Caspase-3

2001

Full-length cDNA of hamster bcl-2 (771 nt) was cloned by RT-PCR and inserted into pGEX-4T-1 to produce the recombinant hamster Bcl-2 protein. The purified recombinant Bcl-2 protein (26.4 kDa) was used as a substrate for the active human caspase-3 and caspase-9 in vitro. It is shown here that Bcl-2 is efficiently cleaved by caspase-3 to a 23 kDa fragment. Although not possessing a putative caspase-9 cleavage site in its sequence, hamster Bcl-2 was also cleaved by caspase-9 into exactly the same 23 kDa cleavage product, indicating that cleavage occurred at the same site. Caspase-3- and caspase-9-mediated cleavage of Bcl-2 was efficiently blocked by caspase-3 (zDEVD) and caspase-9 (zLEHD) inhi…

Probing human beta1- and beta2 -adrenoceptors with domain-specific fusion protein antibodies.

1997

In order to generate antibodies suitable for immunological studies on beta-adrenoceptors constitutively expressed at low levels in cells or tissues we have produced fusion proteins of the amino- and carboxy-terminus, and the second extracellular loop of the human beta 1- or beta 2-adrenoceptors with bacterial glutathione-S-transferase in E. coli. Rabbit antibodies raised against these fusion proteins strongly reacted with intact human beta 1- or beta 2-adrenoceptors in a subtype- and domain-specific manner. Antibodies directed against the second extracellular loop of the beta 1-adrenoceptor reacted stronger with non-denatured receptors and decreased the affinity of the 3H-labelled antagonis…

Targeting of biotinylated compounds to its target tissue using a low-density lipoprotein receptor–avidin fusion protein

2003

The very high binding affinity of avidin to biotin is one of the highest to occur in nature. We constructed a fusion protein composed of avidin and the endocytotic LDL receptor in order to target biotinylated molecules to cells of the desired tissues. In addition to the native avidin, charge-mutated and nonglycosylated avidins were utilized as part of the fusion proteins, in order to modify its properties. All of the fusion protein versions retained the biotin-binding capacity. Although the specificity was not increased, however, fusion proteins composed of natural avidin and nonglycosylated avidin bound most efficiently to the biotinylated ligands. Fluorescence microscopy and atomic force …

The Mazara del Vallo area, a key site to understand the regional geothermal system of Western Sicily

2012

International public administration on the tip of the tongue: language as a feature of representative bureaucracy in the Economic Community of West A…

2020

Recent scholarship shows increasing interest in gender, ethnic or national representation within regional and international organizations. In contrast, language as a criterion of representation has rarely been scrutinized. We argue that this constitutes an important oversight for two reasons: (1) language is an important identity marker; and (2) language regimes in international public administrations can uniquely address representativeness relative to both member states and groups of citizens. Our article explores language representation in the Economic Community of West African States, and pursues a twofold objective: first, it extends the applicability of representative bureaucracy theo…

Precipitation pattern in the Baltic countries under the influence of large-scale atmospheric circulation and local landscape factors

2010

The main objectives of the study are to analyse the mean precipitation pattern in the Baltic countries (Estonia, Latvia, Lithuania) and the influence of local landscape factors on it, to derive a regionalization, i.e. select regions having coherent fluctuations in precipitation, and to analyse relationships between precipitation and characteristics of the large-scale atmospheric circulation. Monthly precipitation data from 123 stations in the Baltic countries from 1966 to 2005 are analysed in relation to 52 landscape variables and 13 circulation variables. The landscape variables characterize landscape around the meteorological stations: land cover, land surface elevation, distance from the…