Search results for "Wester"

Książki poddane w 1841 r. cenzurze prewencyjnej. Przyczynek źródłowy do dziejów bibliotek klasztorów skasowanych na mocy ukazu z 1832 r. w guberniach…

2014

Mutant HRAS as novel target for MEK and mTOR inhibitors.

2015

HRAS is a frequently mutated oncogene in cancer. However, mutant HRAS as drug target has not been investigated so far. Here, we show that mutant HRAS hyperactivates the RAS and the mTOR pathway in various cancer cell lines including lung, bladder and esophageal cancer. HRAS mutation sensitized toward growth inhibition by the MEK inhibitors AZD6244, MEK162 and PD0325901. Further, we found that MEK inhibitors induce apoptosis in mutant HRAS cell lines but not in cell lines lacking RAS mutations. In addition, knockdown of HRAS by siRNA blocked cell growth in mutant HRAS cell lines. Inhibition of the PI3K pathway alone or in combination with MEK inhibitors did not alter signaling nor had an imp…

Ocena współczesnej kultury Zachodu w nauczaniu Benedykta XVI (Josepha Ratzingera)

2021

The Assessment of the Contemporary Western Culture in the Teaching of Benedict XVI (Joseph Ratzinger)
 The article consists of two parts. In the first one, the authors pointed out an argument in the social sciences concerning ability to reasonably use the „culture” concept in scientific discourse. In the second one, they analyzed opinions Benedict XVI ( Joseph Ratzinger) expressed about present condition of the Western culture. Views of this figure were selected to study due to recognizing significant influential role the Pope plays in the public sphere. In the opinion of Benedict XVI, today there are two cultures of the West that coexist: rational Christian culture and Enlightenment (…

Découverte d'Arthropodes et de bivalves inédits dans le Permien continental (Lodévois, France)

1997

Resume Des Triopsides, Conchostraces, Insectes, Bivalves, pistes et terriers d'Arthropodes sont decouverts dans le Groupe saxonien considere longtemps comme quasi azoique. Ces fossiles sont pour la plupart inedits, au niveau de l'espece, de la famille et de l'ordre. Chez les Insectes, de nouveaux taxons comblent des lacunes temporelles considerables, allant du Jurassique au Permien. Les informations recueillies vont permettre de rediscuter certaines phylogenies et de reapprecier l'impact de la crise fini-permienne sur cette classe. La repartition des Insectes et des Conchostraces suggere de donner un âge Koungourien a Tatarien (Leonardien a Capitanien) au ≪ Saxonien ≫.

Ethical Stance and Evolving Technosexual Culture : A Case for Human-Computer Interaction

2021

Issues relating to ethics and how moral principles evolve are imminently engrained in culture. Culture and technology cannot be separated from one another, as both are processes and reflections of social cognition and experience through action and practice. Technology is the embodiment of values and enabler of culture. As technology develops and human relationships to information technology (IT) become ever more intricate and intimate the cultural framework underpinning values and ethics also morphs. The Internet is everywhere and humans are reliant on it for everything from banking to maintaining family relationships. Anything an individual could possibly desire can be found within the mas…

2019

(1) Background: The high-grade neuroepithelial tumor of the central nervous system with BCOR alteration (HGNET-BCOR) is a highly malignant tumor. Preclinical models and molecular targets are urgently required for this cancer. Previous data suggest a potential role of insulin-like growth factor (IGF) signaling in HGNET-BCOR. (2) Methods: The primary HGNET-BCOR cells PhKh1 were characterized by western blot, copy number variation, and methylation analysis and by electron microscopy. The expression of IGF2 and IGF1R was assessed by qRT-PCR. The effect of chemotherapeutics and IGF1R inhibitors on PhKh1 proliferation was tested. The phosphorylation of IGF1R and downstream molecules was assessed …

Analysis of complement C3 activation products in human atherosclerotic lesions.

1991

Abstract Cleavage of the complement C3 protein is essential for complement activation. Saline extracts of human atherosclerotic lesions were examined by various techniques for the presence of C3 cleavage fragments. Crossed intermediate gel immunoelectrophoresis revealed that native C3 was the predominate C3 protein in extracts and that the C3dg fragment was also detected. SDS-PAGE/ Western blot analyses of lesion extracts employing monoclonal antibodies directed at C3c and C3dg fragment determinants demonstrated molecular weight bands corresponding to the known molecular weights of all the physiologic C3 cleavage fragments, except Cab which is known to have a short half-life. After C3, the …

Isolation and Characterization of Pathogen-Bearing Endosomes Enable Analysis of Endosomal Escape and Identification of New Cellular Cofactors of Infe…

2013

Many pathogens, including viruses, bacteria, as well as bacterial toxins, enter their target cells by endocytosis leading to accumulation of pathogenic and cellular proteins in endosomes. Here, we present detailed experimental instructions on isolation of endosomes after virus infection and their subsequent biomolecular characterization. The isolation of endosomes is based on discontinuous sucrose gradient centrifugation, where different endosomal compartments accumulate at a specific sucrose interface. This enables the enrichment and separation of the virus-interacting and co-internalized cell-surface receptors and membrane-associated proteins. The endosomal fractions can be further analyz…

Cigarette smoke alters primary human bronchial epithelial cell (PBEC) differentiation atAir-Liquid Interface (ALI): role of Oct-4, CD146 and CD105

2017

The airway epithelium is a dynamic tissue that undergoes slow but constant renewal. Dysregulation of airway epithelial cell function related to cigarette smoke (CS) exposure plays an important role in the pathophysiology of COPD. Oct-4 is the crucial POU domain transcription factor responsible for maintaining cellular self-renewal and regeneration, and CD146 and CD105 are adhesion molecule involved in cellular proliferation, differentiation, epithelial-mesenchymal transition and tissue remodelling. Bronchial biopsy specimens (BBs) were obtained from 9 healthy controls (C) and 9 COPD. ALI cultures of PBEC from C were exposed to CS extract (CSE) for 7, 14, 21 days. Oct-4, CD105 and CD146 expr…

Monoclonal antibody TeM 106 reacts with a tonoplast intrinsic protein of 106 kDa from Brassica oleracea L

1995

A monoclonal antibody, designated TeM 106, that recognizes an intrinsic protein from the vacuole membrane (tonoplast) of cauliflower (Brassica oleracea L. var. botrytis) is described. Mice were immunized with a tonoplast fraction that had been purified from differentiating meristematic cells from the cauliflower head. Hybridomas were generated and screened by means of Enzyme Linked Immuno Sorbent Assays for differential reactivity to tonoplast over non-related proteins (bovine serum albumin). One out of 14 reactive murine clones was selected on the basis of its stability, secretory efficiency, and high affinity of the secreted antibodies. TeM 106 is an IgM which was shown by indirect immuno…