Search results for "XOS"
showing 10 items of 414 documents
CCDC 1530419: Experimental Crystal Structure Determination
2017
Related Article: Sujoy Das, Himadri Sekhar Sarkar, Md Raihan Uddin, Kari Rissanen, Sukhendu Mandal, Prithidipa Sahoo|2017|Chem.Commun.|53|7600|doi:10.1039/C7CC02935G
CCDC 1427184: Experimental Crystal Structure Determination
2016
Related Article: Moritz Schubert, Kathrin Wehming, Anton Kehl, Martin Nieger, Gregor Schnakenburg, Roland Fröhlich, Dieter Schollmeyer, Siegfried R. Waldvogel|2016|Eur.J.Org.Chem.|2016|60|doi:10.1002/ejoc.201501384
CCDC 1415860: Experimental Crystal Structure Determination
2016
Related Article: Moritz Schubert, Kathrin Wehming, Anton Kehl, Martin Nieger, Gregor Schnakenburg, Roland Fröhlich, Dieter Schollmeyer, Siegfried R. Waldvogel|2016|Eur.J.Org.Chem.|2016|60|doi:10.1002/ejoc.201501384
CCDC 1415862: Experimental Crystal Structure Determination
2016
Related Article: Moritz Schubert, Kathrin Wehming, Anton Kehl, Martin Nieger, Gregor Schnakenburg, Roland Fröhlich, Dieter Schollmeyer, Siegfried R. Waldvogel|2016|Eur.J.Org.Chem.|2016|60|doi:10.1002/ejoc.201501384
CCDC 1415861: Experimental Crystal Structure Determination
2016
Related Article: Moritz Schubert, Kathrin Wehming, Anton Kehl, Martin Nieger, Gregor Schnakenburg, Roland Fröhlich, Dieter Schollmeyer, Siegfried R. Waldvogel|2016|Eur.J.Org.Chem.|2016|60|doi:10.1002/ejoc.201501384
CCDC 1419863: Experimental Crystal Structure Determination
2016
Related Article: Moritz Schubert, Kathrin Wehming, Anton Kehl, Martin Nieger, Gregor Schnakenburg, Roland Fröhlich, Dieter Schollmeyer, Siegfried R. Waldvogel|2016|Eur.J.Org.Chem.|2016|60|doi:10.1002/ejoc.201501384
Levansucrases from Pseudomonas syringae pv. tomato and P. chlororaphis subsp. aurantiaca: Substrate specificity, polymerizing properties and usage of…
2011
Levansucrases of Pseudomonas syringae pv. tomato DC3000 (Lsc3) and Pseudomonas chlororaphis subsp. aurantiaca (also Pseudomonas aurantiaca) (LscA) have 73% identity of protein sequences, similar substrate specificity and kinetic properties. Both enzymes produce levan and fructooligosaccharides (FOS) of varied length from sucrose, raffinose and sugar beet molasses. A novel high-throughput chip-based nanoelectrospray mass spectrometric method was applied to screen alternative fructosyl acceptors for levansucrases. Lsc3 and LscA could both transfructosylate D-xylose, D-fucose, L- and D-arabinose, D-ribose, D-sorbitol, xylitol, xylobiose, D-mannitol, D-galacturonic acid and methyl-α-D-glucopyra…
Elimination of a bacterial pore-forming toxin by sequential endocytosis and exocytosis
2008
Staphylococcus aureus alpha-toxin is the archetype of bacterial pore forming toxins and a key virulence factor secreted by the majority of clinical isolates of S. aureus. Toxin monomers bind to target cells and oligomerize to form small beta-barrel pores in the plasma membrane. Many nucleated cells are able to repair a limited number of lesions by unknown, calcium-independent mechanisms. Here we show that cells can internalize alpha-toxin, that uptake is essential for cellular survival, and that pore-complexes are not proteolytically degraded, but returned to the extracellular milieu in the context of exosome-like structures, which we term toxosomes.
A novel and simple method for the purification of extracellular levansucrase from Zymomonas mobilis.
2003
A new and simple method for the purification of extracellular levansucrase from Zymomonas mobilis from highly viscous fermentation broth was developed. After incubation of the fermentation broth with a fructose-polymer cleaving enzyme preparation (Fructozyme, Novozymes, DK) for 48 h, levansucrase precipitated as aggregates and was redissolved in a 3 M urea solution. By ongoing size-exclusion chromatography on Sephacryl S-300 the final levansucrase preparation was purified 100-fold and exhibited a specific activity of 25-35 U/mg(protein). The levansucrase was stable in 3 M urea solution for at least four months without inactivation. To maximize the enzyme yield the dynamic changes of extrace…
Chronic myeloid leukemia-derived exosomes promote tumor growth through an autocrine mechanism.
2014
Background Chronic myeloid leukemia (CML) is a clonal hematopoietic stem cell disorder in which leukemic cells display a reciprocal t(9:22) chromosomal translocation that results in the formation of the chimeric BCR-ABL oncoprotein, with a constitutive tyrosine kinase activity. Consequently, BCR-ABL causes increased proliferation, inhibition of apoptosis, and altered adhesion of leukemic blasts to the bone marrow (BM) microenvironment. It has been well documented that cancer cells can generate their own signals in order to sustain their growth and survival, and recent studies have revealed the role of cancer-derived exosomes in activating signal transduction pathways involved in cancer cell…