Search results for "Zeatin"
showing 8 items of 8 documents
In vitro propagation of the relict laperinne’s olive (Olea europaea L. subsp. laperrinei)
2017
Olea europaea L. subsp. laperrinei (Oleaceae) is an endemic taxon of the mountainous regions of central Sahara, consisting of currently fragmented and small relict populations. The tree can propagate vegetatively or by seed, but no recent natural regeneration was observed in the Algerian massive populations, some of which are considered threatened with extinction. Sterile triploid individuals were also identified in some populations showing increasing vigour. As a result of its long persistence and despite its rarity, the Laperrine's olive is an iconic component of Saharan mountain ecosystems. The aim of this study is to develop an efficient micropropagation protocol for both diploid and tr…
Micropropagation of globe artichoke (Cynara scolymus L.) from underground dormant buds (“ovoli”)
1996
Side shoots excised from underground dormant buds ofCynara scolymus L. were used as primary explants to establishin vitro cultures. A 3×3 factorial experiment with all possible combinations of three concentrations (0.5, 1.0, 2.0 mg/liter or 2.22, 4.44, 8.88 μM) ofN 6-benzyladenine (BA) and three concentrations (0, 0.1, 0.2 mg/liter or 0, 0.54, 1.07 μM) of 1-naphthaleneacetic acid (NAA) was used to determine the optimum growth regulator combination for shoot multiplication. The highest rate of axillary shoots was induced on Murashige and Skoog agar medium supplemented with 0 mg NAA/liter and 1.0 mg BA/liter (4.44 μM). Other cytokinins tested (kinetin, zeatin, and 2-isopentenyl-adenine were l…
Early embryo achievement through isolated microspore culture in citrus clementina hort. Ex tan., cvs. 'Monreal Rosso' and 'nules'
2015
10 p.-5 fig.-3 tab.
Fruit Regulates Bud Sprouting and Vegetative Growth in Field-Grown Loquat Trees (Eriobotrya japonica Lindl.): Nutritional and Hormonal Changes
2013
The effects of fruit on bud sprouting and vegetative growth were compared on fruiting and defruited loquat trees from fruit set onward. Carbohydrate and nitrogen content in leaves and bark tissues and hormone concentrations were studied during the fruit development and vegetative growth periods. On defruited trees, a significant proportion of buds sprouted in winter, whereas buds from fruiting trees sprouted only in the spring when fruit reached its final size. Furthermore, when panicles were completely removed in autumn, the buds also sprouted. In addition, fruit directly affected vegetative growth by reducing shoot length. An effect of sink removal ( flower or fruit) promoting bud sprouti…
Micropropagation of Genista aetnensis [(Raf. ex Biv.)DC]
2015
Genista aetnensis [(Raf. ex Biv.)DC] is a large deciduous shrub or small tree native to the Italian islands of Sardinia and Sicily. Being winter hardy and characterized by high plasticity in altitude and ecology, the species is grown in gardens and landscaping, both for flower and for its attractive shape. Genista species are generally propagate by seed or semi hardwood cuttings. In this report an efficient in vitro technique for propagation of G. aetnensis was investigated. Multiple shoots were induced on nodal segments of a mature plant of Genista aetnensis . The Murashige and Skoog medium, augmented with different concentrations of benzyladenine either singly or in combination with indol…
Micropropagation of Metrosideros excelsa
2008
Multiple shoots were induced on stem segments of a 8-year-old plant of Metrosideros excelsa Sol ex Gaertn. ‘Parnel’. Axillary shoots produced on uncontaminated explants were excised, segmented and recultured in the same medium to increase the stock of shoot cultures. The Murashige and Skoog (MS) medium, augmented with different concentrations of 2- isopenthenyladenine (2iP) and indole-3-acetic acid (IAA), either singly or in combinations, as potential medium for shoot multiplication by nodal segments was tested. In the following experiment equal molar concentrations of four cytokinins [2iP, kinetin, zeatin and N6-benzyladenine (BA)] in combination with equal molar concentrations of three au…
Improved micropropagation in Polygala myrtifolia
2004
Stem segments from apical shoot tips of Polygala myrtifolia were used as primary explants to establish in vitro cultures. Axillary shoots produced on noncontaminated explants were excised and recultured in the same medium to increase the stock of shoot cultures. Equal molar concentrations of five cytokinins 2-isopentenyladenine (2iP), kinetin, zeatin, N6-benzyladenine (BA) and adenine were tested for ability to induce axillary shoot development from double node stem segments. The highest rate of axillary shoot proliferation was induced on Murashige and Skoog agar medium supplemented with 1.8 M BA. Seven indole-3-acetic acid (IAA) concentrations (2.8, 5.7, 8.6, 11.4, 14.3, 17.1 M) were t…
In vitroplant regeneration and gene transfer in the wild tomatoLycopersicon cheesmanii
2001
SummaryThis paper reports on protocols for in vitro plant regeneration, gene transfer and on shoot salt tolerance from two accessions of L. cheesmanii (Riley). Shoot organogenesis was achieved on media with zeatin or BA/IAA combinations. The two accessions tested, LA1401 and LA530, showed differences in both their aptitude to regenerate and their salt tolerance in vitro. The accession LA530 had a higher organogenic potential than LA1401 (97% vs. 80% of organogenic explants). In contrast, none of the LA530 isolated shoots rooted when cultured in full strength MS-modified medium supplemented with 200.mM NaCl while 93% of shoots from LA1401 formed roots. Finally, Agrobacterium-mediated transfo…