Search results for "acriflavine"

Determination of nitrite by inhibition of the chemiluminescence of acriflavine in a flow-injection assembly

2001

The indirect determination of nitrite was performed with a flow-injection assembly on the basis of the inhibition of the analytical output obtained in a luminometer by oxidation of acriflavine. The acriflavine solution merged with the nitrite and the resulting mixture was injected into a pure water stream. This solution merged with the oxidant solution (potassium permanganate in sulfuric acid medium) and the resulting chemiluminiscence was affected (inhibited) by the presence of nitrite after reaction with the aminoacridine. The method was applicable over the range 10–800 μg l−1 of nitrite with a correlation coefficient of 0.9960. The relative standard deviation was 1.4% and the throughput …

In Vivo Imaging of Enteric Neuronal Networks in Humans Using Confocal Laser Endomicroscopy

2012

Flow-injection analysis study of the chemiluminescent behaviour of proflavine and acriflavine

2001

Abstract The chemiluminescent behaviour of the amino acridines like acriflavine, and proflavin is reported. Different strong oxidants (potassium permanganate, cerium(IV), hexacyanoferrate(III), hydrogen peroxide in different media) were tested, and potassium permanganate in sulphuric acid medium was selected. The study of the experimental parameters affecting the oxidation and detection was performed with the aid of a FIA assembly. The calibration graph was applied over the range 0.05–12.0 μg ml −1 of acriflavine (regression coefficient 0.9957, and the calculated relative standard deviation (R.S.D., %) was 0.9; LOD 10 ng ml −1 and the sample throughput 48 h −1 . The study of interfering com…

In vivo confocal laser laparoscopy allows real time subsurface microscopy in animal models of liver disease.

2007

Background/Aims Histopathology is essential in the diagnostic workup of most liver diseases. However, biopsy sampling might carry risks, is subject to sampling error, and does not provide dynamic tissue imaging. Therefore a newly developed miniaturised confocal probe was evaluated for in vivo microscopic imaging in rodent models of human liver diseases. Methods The handheld laparoscopy probe used a 488nm single line laser for fluorophore excitation. Optical slice thickness was 7μm, lateral resolution 0.7μm. Imaging depth was 0–250μm below the tissue surface. Imaging using different fluorescent staining protocols was performed in healthy mice, IFNγ- and IL-12-induced hepatitis, after bile du…

Diagnosing Helicobacter pylori in vivo by confocal laser endoscopy.

2005

Background & Aims: Confocal laser endomicroscopy enables subsurface microscopic imaging of living tissue during ongoing endoscopy. This case report describes the in vivo detection of Helicobacter pylori by endomicroscopy.Methods: Endomicroscopy (Pentax, Tokyo, EC-3870CIFK) was performed by using two different contrast stains: Topical Acriflavine in addition to intravenously applied fluorescein netted the surface and allowed identification of focal accumulation of Helicobacter pylori at the surface and in deeper layer of the gastric epithelium. Biopsies were performed at the antrum and corpus for urease testing and histology. In addition, biopsies were cultured for Helicobacter pylori. Cultu…

Listeria phage and phage tail induction triggered by components of bacterial-growth media (phosphate, LiCl, nalidixic acid and acriflavine)

2015

ABSTRACT The detection of Listeria monocytogenes from food is currently carried out using a double enrichment. For the ISO methodology, this double enrichment is performed using half-Fraser and Fraser broths, in which the overgrowth of L. innocua can occur in samples where both species are present. In this study, we analyzed the induction of phages and phage tails of Listeria spp. in these media and in two brain heart infusion (BHI) broths (BHIM [bioMérieux] and BHIK [Biokar]) to identify putative effectors. It appears that Na 2 HPO 4 at concentrations ranging from 1 to 40 g/liter with an initial pH of 7.5 can induce phage or phage tail production of Listeria spp., especially with 10 g/lite…

Confocal laser endoscopy for diagnosing intraepithelial neoplasias and colorectal cancer in vivo

2004

Background & Aims: Aconfocal laser endoscopy system has recently been developed that may allow subsurface imaging of living cells in colonic tissue in vivo. The aim of the present study was to assess its potential for prediction of histology during screening colonoscopy for colorectal cancer. Methods: Twenty-seven patients underwent colonoscopy with the confocal endoscope using acriflavine hydrochloride or fluorescein sodium with blue laser illumination. Furthermore, 42 patients underwent colonoscopy with this system using fluorescein sodium. Standardized locations and circumscript lesions were examined by confocal imaging before taking biopsy specimens. Confocal images were graded accordin…

Confocal laser endomicroscopy.

2005

A miniaturized confocal microscope was developed that could be integrated in the distal tip of a conventional colonoscope. With this technique, denoted confocal endomicroscopy, subsurface analysis of the gut mucosa and in-vivo histology during ongoing endoscopy are possible in full resolution by point scanning laser analysis. The diagnostic spectrum of confocal endomicroscopy is expanding from screening and surveillance for colorectal cancer to Barrett's esophagus, Helicobacter pylori-associated gastritis, and gastric cancer. The new detailed images seen with confocal laser endomicroscopy allow a unique look on cellular structures at and below the surface of the gut. This review describes t…