Search results for "actin"
showing 10 items of 1375 documents
Myosin VIIa, harmonin and cadherin 23, three Usher I gene products that cooperate to shape the sensory hair cell bundle
2002
Deaf-blindness in three distinct genetic forms of Usher type I syndrome (USH1) is caused by defects in myosin VIIa, harmonin and cadherin 23. Despite being critical for hearing, the functions of these proteins in the inner ear remain elusive. Here we show that harmonin, a PDZ domain-containing protein, and cadherin 23 are both present in the growing stereocilia and that they bind to each other. Moreover, we demonstrate that harmonin b is an F-actin-bundling protein, which is thus likely to anchor cadherin 23 to the stereocilia microfilaments, thereby identifying a novel anchorage mode of the cadherins to the actin cytoskeleton. Moreover, harmonin b interacts directly with myosin VIIa, and i…
The glycosyltransferase activities of lysyl hydroxylase 3 (LH3) in the extracellular space are important for cell growth and viability.
2008
Abstract Lysyl hydroxylase (LH) isoform 3 is a post-translational enzyme possessing LH, collagen galactosyltransferase (GT) and glucosyltransferase (GGT) activities. We have demonstrated that LH3 is found not only intracellularly, but also on the cell surface and in the extracellular space, suggesting additional functions for LH3. Here we show that the targeted disruption of LH3 by siRNA causes a marked reduction of both glycosyltransferase activities, and the overexpression of LH3 in HT-1080 cells increases hydroxylation of lysyl residues and the subsequent galactosylation and glucosylation of hydroxylysyl residues. These data confirm the multi-functionality of LH3 in cells. Furthermore, t…
Detection of cytokeratin dynamics by time-lapse fluorescence microscopy in living cells.
1999
To monitor the desmosome-anchored cytokeratin network in living cells fusion protein HK13-EGFP consisting of human cytokeratin 13 and the enhanced green fluorescent protein was stably expressed in vulvar carcinoma-derived A-431 cells. It is shown for A-431 subclone AK13-1 that HK13-EGFP emits strong fluorescence in fixed and living cells, being part of an extended cytoplasmic intermediate filament network that is indistinguishable from that of parent A-431 cells. Biochemical, immunological and ultrastructural analyses demonstrate that HK13-EGFP behaves identically to the endogenous cytokeratin 13 and is therefore a reliable in vivo tag for this polypeptide and the structures formed by it. T…
Phylogenetic Position of the Hexactinellida Within the Phylum Porifera Based on the Amino Acid Sequence of the Protein Kinase C from Rhabdocalyptus d…
1998
Recent analyses of genes encoding proteins typical for multicellularity, especially adhesion molecules and receptors, favor the conclusion that all metazoan phyla, including the phylum Porifera (sponges), are of monophyletic origin. However, none of these data includes cDNA encoding a protein from the sponge class Hexactinellida. We have now isolated and characterized the cDNA encoding a protein kinase C, belonging to the C subfamily (cPKC), from the hexactinellid sponge Rhabdocalyptus dawsoni. The two conserved regions, the regulatory part with the pseudosubstrate site, the two zinc fingers, and the C2 domain, as well as the catalytic domain were used for phylogenetic analyses. Sequence al…
Integrating Decomposers, Methane-Cycling Microbes and Ecosystem Carbon Fluxes Along a Peatland Successional Gradient in a Land Uplift Region
2021
AbstractPeatlands are carbon dioxide (CO2) sinks that, in parallel, release methane (CH4). The peatland carbon (C) balance depends on the interplay of decomposer and CH4-cycling microbes, vegetation, and environmental conditions. These interactions are susceptible to the changes that occur along a successional gradient from vascular plant-dominated systems to Sphagnum moss-dominated systems. Changes similar to this succession are predicted to occur from climate change. Here, we investigated how microbial and plant communities are interlinked with each other and with ecosystem C cycling along a successional gradient on a boreal land uplift coast. The gradient ranged from shoreline to meadows…
Genesis of the Lower Triassic bonebeds from Gogolin (S Poland): The impact of microbial mats on trapping of vertebrate remains
2017
Three bone-bearing horizons, consis ting of seven bone-bearing beds with fi sh and reptile remains, were recently discovered in the Uppermost Röt (Buntsandstein, Lower Tri assic) peritidal and shallow marine carbonates in the vi- cinity of Gogolin (S Poland). The aim of this study is to reco gnize the genesis and deposi tional environments of the bonebeds. Detailed fi eldwork, microfacies analysis, and SEM-EDS anal ysis reveal that the vertebrate remains occur to a great extent alongside evidence for former microbial activity. The reptile remains represent at least Dactylosaurus and Nothosaurus (Sauropterygia) genera, while the fi sh remains belong to the Chondrichthyes and Actinopterygii. …
Dark Matter Search Results from a One Ton-Year Exposure of XENON1T
2018
We report on a search for Weakly Interacting Massive Particles (WIMPs) using 278.8 days of data collected with the XENON1T experiment at LNGS. XENON1T utilizes a liquid xenon time projection chamber with a fiducial mass of $(1.30 \pm 0.01)$ t, resulting in a 1.0 t$\times$yr exposure. The energy region of interest, [1.4, 10.6] $\mathrm{keV_{ee}}$ ([4.9, 40.9] $\mathrm{keV_{nr}}$), exhibits an ultra-low electron recoil background rate of $(82\substack{+5 \\ -3}\textrm{ (sys)}\pm3\textrm{ (stat)})$ events/$(\mathrm{t}\times\mathrm{yr}\times\mathrm{keV_{ee}})$. No significant excess over background is found and a profile likelihood analysis parameterized in spatial and energy dimensions exclude…
Über den Bau des Saccus vasculosus einiger Tiefseefische
1955
Die aus der Tiefsee stammenden, hier untersuchten Pediculaten (Oneirodes niger, Gigantactis Vanhoeffeni, Aceratias macrorhinus indicus und Halicmetus ruber) besitzen ausnahmslos einen Saccus vasculosus.
Hypogonadism in β-thalassemic adolescents: A characteristic pituitary-gonadal impairment. The ineffectiveness of long-term iron chelation therapy
1990
The pituitary-gonadal function was studied in 18 beta-thalassemic female adolescents, 8 with delayed puberty and 10 with primary amenorrhea,treated with repeated transfusions and long-term iron chelation therapy by subcutaneous infusion. A 100 micrograms gonadotropin-releasing hormone (GnRH) test, a double-bolus GnRH test after estradiol administration in non-responders, a 400 micrograms thyrotropin-releasing hormone (TRH) test and a 'high dose' human menopausal gonadotropin (hMG) test were performed. LH and FSH peak levels were significantly lower in thalassemic patients than in controls, both in the 100 micrograms GnRH test (LH was 4.3 +/- 0.7 mIU/ml vs 40.8 +/- 6.0 mIU/ml and FSH 3.3 +/-…