Search results for "actin"
showing 10 items of 1375 documents
Detection of the Lowest-Lying Odd-Parity Atomic Levels in Actinium
2020
Two lowest-energy odd-parity atomic levels of actinium, 7s27pP21/2o, 7s27pP23/2o, were observed via two-step resonant laser-ionization spectroscopy and their respective energies were measured to be 7477.36(4) and 12 276.59(2) cm-1. The lifetimes of these states were determined as 668(11) and 255(7) ns, respectively. In addition, we observed the effect of the hyperfine structure on the line for the transition to P23/2o. These properties were calculated using a hybrid approach that combines configuration interaction and coupled-cluster methods, in good agreement with the experiment. The data are of relevance for understanding the complex atomic spectra of actinides and for developing efficien…
Immunodetection of the microvillous cytoskeleton molecules villin and ezrin in the parasitophorous vacuole wall of Cryptosporidium parvum (Protozoa: …
1999
Microvilli - actin - villin - ezrin - Cryptosporidium parvum The sporozoites and merozoites of the Apicomplexan protozoan Cryptosporidium parvum (C. parvum) invade the apical side of enterocytes and induce the formation of a parasitophorous vacuole which stays in the brush border area and disturbs the distribution of microvilli. The vacuole is separated from the apical cytoplasm of the cell by an electron-dense layer of undetermined composition. In order to characterize the enterocyte cytoskeleton changes that occur during C. parvum invasion and development, we used both confocal immunofluorescence and immunoelectron microscopy to examine at the C.parvum-enterocyte interface the distributio…
The entangled relationship between cilia and actin
2020
Primary cilia are microtubule-based sensory cell organelles that are vital for tissue and organ development. They act as an antenna, receiving and transducing signals, enabling communication between cells. Defects in ciliogenesis result in severe genetic disorders collectively termed ciliopathies. In recent years, the importance of the direct and indirect involvement of actin regulators in ciliogenesis came into focus as it was shown that F-actin polymerisation impacts ciliation. The ciliary basal body was further identified as both a microtubule and actin organising centre. In the current review, we summarize recent studies on F-actin in and around primary cilia, focusing on different acti…
Arsenic trioxide alters the differentiation of mouse embryonic stem cell into cardiomyocytes
2015
AbstractChronic arsenic exposure is associated with increased morbidity and mortality for cardiovascular diseases. Arsenic increases myocardial infarction mortality in young adulthood, suggesting that exposure during foetal life correlates with cardiac alterations emerging later. Here, we investigated the mechanisms of arsenic trioxide (ATO) cardiomyocytes disruption during their differentiation from mouse embryonic stem cells. Throughout 15 days of differentiation in the presence of ATO (0.1, 0.5, 1.0 μM) we analysed: the expression of i) marker genes of mesoderm (day 4), myofibrillogenic commitment (day 7) and post-natal-like cardiomyocytes (day 15); ii) sarcomeric proteins and their orga…
RNA sequencing-based transcriptome profiling of cardiac tissue Implicados novela putative disease mechanisms in FLNC-associated arrhythmogenic cardio…
2020
Arrhythmogenic cardiomyopathy (ACM) encompasses a group of inherited cardiomyopathies including arrhythmogenic right ventricular cardiomyopathy (ARVC) whose molecular disease mechanism is associated with dysregulation of the canonical WNT signalling pathway. Recent evidence indicates that ARVC and ACM caused by pathogenic variants in the FLNC gene encoding filamin C, a major cardiac structural protein, may have different molecular mechanisms of pathogenesis. We sought to identify dysregulated biological pathways in FLNC-associated ACM. RNA was extracted from seven paraffin-embedded left ventricular tissue samples from deceased ACM patients carrying FLNC variants and sequenced. Transcript le…
Dynamic force sensing of filamin revealed in single-molecule experiments
2012
Mechanical forces are important signals for cell response and development, but detailed molecular mechanisms of force sensing are largely unexplored. The cytoskeletal protein filamin is a key connecting element between the cytoskeleton and transmembrane complexes such as integrins or the von Willebrand receptor glycoprotein Ib. Here, we show using single-molecule mechanical measurements that the recently reported Ig domain pair 20–21 of human filamin A acts as an autoinhibited force-activatable mechanosensor. We developed a mechanical single-molecule competition assay that allows online observation of binding events of target peptides in solution to the strained domain pair. We find that fi…
Discrimination between stationary objects by the blind cave fishAnoptichthys jordani (Characidae)
1981
Blind Mexican cave fish were trained to discriminate between grids of vertical bars placed at various distances from each other. Discrimination was possible only if the freely swimming fish was allowed to glide past the grids in close proximity.
Residues of planar polychloroaromatic compounds in Baltic fish and seal
1990
Abstract Polychlorinated dibenzo-p-dioxins, dibenzofurans, coplanar PCBs and other dioxin-type of toxins have been analyzed in Baltic salmon, cod, ringed seal and grey seal and in Arctic cod.
Corrigendum to: When phenotypes fail to illuminate underlying genetic processes in fish and fisheries science
2021
The food and parasites of fish in some deep basins of northern L. Päijänne
1982
The composition of the fish stock, food and ‘macroparasites’ were studied in eleven basins (22–100 m) of Lake Jyvasjarvi and North Paijanne in August–September 1976. The fishing was done by means of a series of nets (meshes 15, 21 and 35 mm) laid on the bottom overnight. No fish were found in the two northernmost basins owing to bad oxygen conditions caused by waste waters. Smelt and burbot were the most abundant fish in the catches in other basins but the vendace was rare. Relict crustaceans and some ‘deep water’ copepods (e.g. Heterocope borealis) played an important role in the food of fish in all basins.